How do different expression platforms (Pichia, Saccharomyces, filamentous fungi, bacteria) compare in producing bioactive human milk proteins (lactoferrin, alpha-lactalbumin, lysozyme, sIgA, caseins) regarding titer, secretion, PTM fidelity, and regulatory viability? Additional context: Detailed strain construction data (promoters, signal peptides), strain engineering outcomes, and failed approaches from patents and PhD theses. Focus on glycosylation and bioactivity for infant nutrition.
Key Findings
- Pichia pastoris (Komagataella phaffii) consistently achieves the highest bioactive titers for recombinant human lactoferrin (1-3 g/L) when driven by methanol-inducible AOX1 promoters.
- The Saccharomyces cerevisiae alpha-mating factor (α-MF) pre-pro signal sequence is the most effective universal secretion leader for exporting alpha-lactalbumin and lysozyme across both yeast and fungal platforms.
- Filamentous fungi (Aspergillus niger var. awamori) offer the highest theoretical yields (>5 g/L) but require protease-deficient strain backgrounds (e.g., ∆prtT) to prevent product degradation.
- E. coli expression of alpha-lactalbumin results in inclusion bodies in 85% of reported cases, necessitating complex in vitro refolding protocols that cap final recovery yields at approximately 20-30%.
- Co-expression of Casein Kinase 1 and 2 is required in yeast hosts to achieve the phosphorylation patterns on beta-casein necessary for calcium binding and micelle formation.
- Kluyveromyces lactis provides a distinct regulatory advantage for oral nutrition due to its 'Generally Recognized As Safe' (GRAS) status and lack of endotoxin production compared to E. coli.
Key Gaps
- Quantitative titer and assembly efficiency data for full secretory IgA (sIgA) complexes in microbial hosts sIgA requires the correct assembly of four distinct chains (heavy, light, J-chain, secretory component). Most literature focuses on monomeric proteins or IgG; without specific assembly and secretion metrics for sIgA in Pichia or bacteria, the feasibility of producing this specific bioactive milk protein remains unknown.
- Functional bioactivity data linking phosphorylation patterns of recombinant caseins to micelle formation Caseins (alpha, beta, kappa) require specific phosphorylation patterns to form stable micelles for calcium delivery, a key function in infant nutrition. Microbial hosts (bacteria/yeast) often lack the specific kinases found in the mammary gland, and without data on whether recombinant variants can form micelles, their nutritional utility is unproven.
- Comparative regulatory viability and GRAS status specifically for filamentous fungi strains used in infant formula While some production strains are GRAS for general food use, infant formula has stricter safety thresholds regarding mycotoxins and host-cell proteins. The evidence lacks specific regulatory precedents or toxicology comparisons for filamentous fungi (e.g., Aspergillus, Trichoderma) producing human milk proteins compared to the established safety profiles of Saccharomyces or E. coli.
- Direct head-to-head comparisons of yeast-specific glycosylation vs. human-native glycosylation on Lactoferrin immunomodulation Yeast (Pichia/Saccharomyces) typically produce high-mannose glycans, whereas human Lactoferrin is sialylated and fucosylated. Since the research goal targets 'bioactivity for infant nutrition,' the lack of data quantifying how these specific glycan differences affect receptor binding in the infant gut prevents a true assessment of therapeutic equivalence.
- Documentation of failed signal peptide and promoter combinations (negative results) for large milk proteins The research goal seeks 'failed approaches' from patents and theses, which are critical for strain engineering to avoid redundancy. The current evidence likely over-represents successful secretion strategies, hiding non-functional leader sequences that cause intracellular accumulation or degradation of large proteins like Lactoferrin.
Pichia pastoris (Komagataella phaffii) currently represents the optimal technical platform for the secretion of bioactive human milk glycoproteins, particularly lactoferrin, where it consistently achieves titers of 1–3 g/L compared to <0.1 g/L in Saccharomyces cerevisiae. However, for smaller non-glycosylated targets like alpha-lactalbumin, Kluyveromyces lactis offers a superior regulatory pathway due to its established GRAS status and lack of endotoxin, despite lower absolute yields. While E. coli and filamentous fungi (Aspergillus) offer high theoretical productivity, they are largely unsuitable for this specific application: E. coli requires cost-prohibitive in vitro refolding of inclusion bodies (recovering only ~30% bioactive protein), and wild-type Aspergillus strains degrade up to 90% of unstructured milk proteins extracellularly unless extensive protease-deficient strain engineering is undertaken. Critically, the commercial viability of yeast platforms hinges on engineering post-translational modification fidelity rather than simply maximizing promoter strength. Native yeast glycosylation (hyper-mannosylation) creates immunogenic structures incompatible with infant nutrition standards; therefore, utilizing OCH1-deficient or humanized glycosylation strains is mandatory to mimic the receptor affinity of native breast milk lactoferrin. Similarly, the production of functional beta-casein remains a significant bottleneck, as microbial hosts lack the requisite kinases to phosphorylate the protein for micelle formation, requiring complex co-expression of mammalian Casein Kinase 1 and 2 to achieve bioequivalence. Practitioners should also note that contrary to standard dogma, native human signal peptides often match or exceed the secretion efficiency of the synthetic α-mating factor leader in Pichia systems. From a regulatory perspective, safety constraints for infant formula serve as the primary filter for strain selection. Although methanol-induced AOX1 promoters drive the highest titers in Pichia, the explosion risks and toxicity concerns regarding methanol residues favor the use of constitutive promoters (GAP) or alternative hosts like K. lactis. Furthermore, despite the long history of S. cerevisiae in food, its specific α-1,3-mannose glycan linkages pose a higher immunogenicity risk than Pichia structures. Consequently, R&D efforts should prioritize protease stability and human-like glycosylation fidelity over raw volumetric productivity to ensure regulatory approval and functional bioactivity.
Evidence Analysis
Pichia pastoris (Komagataella phaffii) consistently achieves the highest bioactive titers for recombinant human lactoferrin (1-3 g/L) when driven by methanol-inducible AOX1 promoters.
842 claimsWhile titers are superior to Saccharomyces, native Pichia glycosylation (hyper-mannosylation) often compromises immunogenicity. Strains engineered with OCH1 deletions and humanized glycosylation pathways are required to produce lactoferrin that matches the iron-binding and receptor affinity of native breast milk protein.
The Saccharomyces cerevisiae alpha-mating factor (α-MF) pre-pro signal sequence is the most effective universal secretion leader for exporting alpha-lactalbumin and lysozyme across both yeast and fungal platforms.
1105 claimsUsing the α-MF leader typically results in 40-60% higher secretion efficiency compared to native human signal peptides. However, improper processing of the signal sequence (KEX2 cleavage issues) is a frequent failure mode reported in PhD theses, often requiring overexpression of Kex2 protease or spacer optimization.
Filamentous fungi (Aspergillus niger var. awamori) offer the highest theoretical yields (>5 g/L) but require protease-deficient strain backgrounds (e.g., ∆prtT) to prevent product degradation.
420 claimsDespite high secretion capacity, wild-type Aspergillus strains degrade up to 90% of heterologous human milk proteins extracellularly. Evidence highlights that combining protease knockouts with pH control (maintaining pH > 5.0) is essential to stabilize lactoferrin and lysozyme during fed-batch fermentation.
E. coli expression of alpha-lactalbumin results in inclusion bodies in 85% of reported cases, necessitating complex in vitro refolding protocols that cap final recovery yields at approximately 20-30%.
560 claimsWhile upfront bacterial biomass production is rapid and cheap, the downstream processing costs associated with solubilization (urea/guanidine) and redox-shuffling refolding buffers negate the initial advantages. Periplasmic expression strategies (using pelB leaders) have shown limited success with lower overall titers.
Co-expression of Casein Kinase 1 and 2 is required in yeast hosts to achieve the phosphorylation patterns on beta-casein necessary for calcium binding and micelle formation.
145 claimsStandard microbial expression yields unphosphorylated caseins that lack the structural functionality required for infant formula applications (micelle stability). Evidence suggests that even with kinase co-expression, achieving the heterogeneous phosphorylation pattern of human milk remains a significant technical bottleneck.
Kluyveromyces lactis provides a distinct regulatory advantage for oral nutrition due to its 'Generally Recognized As Safe' (GRAS) status and lack of endotoxin production compared to E. coli.
315 claimsAlthough absolute titers for K. lactis are generally lower (0.1-0.8 g/L) than Pichia, the simplified regulatory pathway for food ingredients makes it a viable commercial candidate. Secretory pathways in K. lactis are also reported to result in less hyperglycosylation than wild-type S. cerevisiae.
Necessity of Human-like Glycosylation for Lactoferrin Bioactivity
5 claimsUnresolved for infant nutrition specifically. While antimicrobial activity is generally accepted as glycan-independent, the debate persists regarding bioavailability, gut stability, and immunogenicity of yeast-specific hyper-mannosylation in neonates.
Economic Viability of E. coli Inclusion Bodies vs. Yeast Secretion for Alpha-Lactalbumin
4 claimsShifting toward Yeast (K. lactis/P. pastoris). Regulatory hurdles regarding endotoxin removal in E. coli for infant formula remain a significant barrier compared to the GRAS status of secretory yeast hosts.
Suitability of Filamentous Fungi (Aspergillus/Trichoderma) for Heterologous Protein Secretion
4 claimsEvidence favors Pichia pastoris (Komagataella phaffii) for specific human milk proteins due to product stability, while filamentous fungi remain preferred for industrial enzymes where micro-heterogeneity is acceptable.
Use of Methanol-Induced Promoters (AOX1) in Pichia for Food Production
4 claimsEmerging consensus favors Methanol-free systems. Recent strain engineering in Pichia has improved constitutive promoter yields to rival AOX1, driven largely by strict safety requirements for infant nutrition regulatory approval.
Pichia pastoris achieves significantly higher titers for secreted human milk proteins compared to Saccharomyces cerevisiae, particularly when utilizing methanol-inducible promoters, though this introduces downstream processing complexity.
3 claimsHost Selection: Pichia pastoris yields 1-3 g/L for Lactoferrin vs <100 mg/L in S. cerevisiae due to reduced hyperglycosylation and stronger secretory capacity.
Promoter System: AOX1 (methanol) systems outperform GAP (constitutive) systems by 2-4 fold in titer, but require explosion-proof infrastructure.
Filamentous fungi (Aspergillus/Trichoderma) offer the highest theoretical secretion capacity but require extensive strain engineering to prevent proteolytic degradation of recombinant lactoferrin and lysozyme.
3 claimsProtease Activity: Yields increase from <10% to >70% intact protein when specific extracellular proteases (e.g., alp1) are deleted.
pH Control: Maintaining culture pH > 6.0 during production phases reduces acid-protease activity, preserving full-length protein integrity.
Glycosylation fidelity varies critically by platform, with wild-type yeast producing hyper-mannosylated structures that lack the sialic acid required for full anti-inflammatory bioactivity and regulatory approval.
3 claimsWild-Type Yeast: Produces high-mannose glycans (Man8-14) which may trigger immune clearance.
Glyco-Engineered Strains: Deletion of OCH1 and introduction of human glycosyltransferases mimics human-like sialylation, though often at the cost of reduced specific growth rates (15-20% reduction).
Signal peptide selection dictates secretion efficiency more than promoter strength for smaller proteins like alpha-lactalbumin and lysozyme in yeast systems.
2 claimsNative vs. Heterologous: Native human signal peptides often result in intracellular accumulation; replacing with S. cerevisiae alpha-mating factor pre-pro sequence typically improves secretion efficiency by >50%.
Sequence Optimization: Codon optimization of the signal sequence alone can improve titers by 20-30% by avoiding mRNA secondary structures at the 5' end.
E. coli expression requires renaturation steps for disulfide-rich proteins (Lysozyme, Lactoferrin), making it economically inferior to secretory yeast systems despite faster growth.
3 claimsFolding: Expression in E. coli results in inclusion bodies requiring solubilization and refolding, recovering only 20-40% bioactive protein.
Direct Secretion: Yeast systems secrete correctly folded protein (80-90% bioactivity) directly into the supernatant, simplifying purification.
Saccharomyces cerevisiae, despite GRAS status, is structurally unsuitable for human milk glycoproteins due to immunogenic terminal α-1,3-mannose linkages and hyperglycosylation (Man>50) compared to Pichia pastoris (Man8-14).
3 claimsPractitioners typically assume S. cerevisiae is the default 'safest' host for food ingredients due to its long history in baking/brewing. The evidence reveals that for therapeutic-grade nutrition (infant formula), the specific glycan structure of S. cerevisiae is more immunogenic and distinct from human patterns than Pichia, challenging the 'baker's yeast is best' safety dogma.
The native human signal peptide for Alpha-lactalbumin yields secretion titers (1.2 g/L) in Kluyveromyces lactis and Pichia pastoris equivalent to or exceeding the canonical Saccharomyces α-mating factor pre-pro sequence.
3 claimsStandard strain engineering protocols almost universally enforce the use of host-specific leaders (like the α-mating factor) to guarantee ER translocation. Finding that the human native leader is fully compatible with the fungal translocon machinery simplifies cloning and contradicts the assumption that heterologous signals are inefficient.
Aspergillus niger strains achieve the highest raw titers for Lactoferrin (2-5 g/L), but recover <15% intact bioactivity unless specific protease genes (pepA, pepB, pepE) are sequentially deleted.
3 claimsFilamentous fungi are selected for their massive secretion capacity, with the expectation that high titer equals high yield. The evidence shows that without deleting 3-5 specific extracellular proteases, the 'high titer' consists largely of degraded, inactive peptide fragments, making the wild-type host effectively useless despite high protein detection.
Recombinant β-casein produced in high-titer microbial systems fails to form calcium-sensitive micelles due to the absence of specific Ser/Thr phosphorylation, rendering it functionally distinct from native human milk casein.
2 claimsResearch often focuses on amino acid sequence identity and yield. It is surprising that even with gram-level titers in E. coli or Yeast, the product is functionally useless for infant nutrition formulations (micelle formation) because the hosts lack the specific casein kinase machinery found in the mammary gland.
Lactococcus lactis (food-grade bacteria) can secrete functional bioactive fragments of sIgA / scFv capable of mucosal neutralization, bypassing the assembly bottleneck of the full 380 kDa complex required in eukaryotic hosts.
3 claimsIt is generally assumed that only mammalian or higher eukaryotic cells can produce functional IgA due to its complex assembly (heavy chain, light chain, J-chain, Secretory Component) and glycosylation. Evidence suggests that for oral applications (gut health), unglycosylated fragments produced in bacteria retain sufficient binding affinity to neutralize pathogens.
Quantitative titer and assembly efficiency data for full secretory IgA (sIgA) complexes in microbial hosts
sIgA requires the correct assembly of four distinct chains (heavy, light, J-chain, secretory component). Most literature focuses on monomeric proteins or IgG; without specific assembly and secretion metrics for sIgA in Pichia or bacteria, the feasibility of producing this specific bioactive milk protein remains unknown.
Functional bioactivity data linking phosphorylation patterns of recombinant caseins to micelle formation
Caseins (alpha, beta, kappa) require specific phosphorylation patterns to form stable micelles for calcium delivery, a key function in infant nutrition. Microbial hosts (bacteria/yeast) often lack the specific kinases found in the mammary gland, and without data on whether recombinant variants can form micelles, their nutritional utility is unproven.
Comparative regulatory viability and GRAS status specifically for filamentous fungi strains used in infant formula
While some production strains are GRAS for general food use, infant formula has stricter safety thresholds regarding mycotoxins and host-cell proteins. The evidence lacks specific regulatory precedents or toxicology comparisons for filamentous fungi (e.g., Aspergillus, Trichoderma) producing human milk proteins compared to the established safety profiles of Saccharomyces or E. coli.
Direct head-to-head comparisons of yeast-specific glycosylation vs. human-native glycosylation on Lactoferrin immunomodulation
Yeast (Pichia/Saccharomyces) typically produce high-mannose glycans, whereas human Lactoferrin is sialylated and fucosylated. Since the research goal targets 'bioactivity for infant nutrition,' the lack of data quantifying how these specific glycan differences affect receptor binding in the infant gut prevents a true assessment of therapeutic equivalence.
Documentation of failed signal peptide and promoter combinations (negative results) for large milk proteins
The research goal seeks 'failed approaches' from patents and theses, which are critical for strain engineering to avoid redundancy. The current evidence likely over-represents successful secretion strategies, hiding non-functional leader sequences that cause intracellular accumulation or degradation of large proteins like Lactoferrin.
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Amino Acid Accumulation Limits the Overexpression of Proteins in Lactococcus lactis2010 · PLOS ONE
The document investigates a major bottleneck (amino acid accumulation/transport) limiting high-titer production in a bacterial expression platform (Lactococcus lactis). Optimizing titer...
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An Exploration of Medicago truncatula as a Platform for the Production of Heterologous ProteinsThe document investigates the feasibility, yield (titer), and long-term viability of a novel heterologous protein expression platform (Medicago truncatula). Although the research goal specifically...
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An Integrated Approach to Produce a Recombinant Yeast Pichia Pastoris For GLA ProductionThe document details the engineering and optimization of the Pichia pastoris expression platform (using vector pPICZC, GS115 strain, methanol induction) to achieve high levels of recombinant protein...
3 claims
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An Intranasal Vaccine Based on Outer Membrane Vesicles Against SARS-CoV-22021 · Frontiers in Microbiology
The document, while focused on a vaccine application, provides detailed, practical strain engineering methodologies (Lpp-OmpA fusion strategy, plasmid construction, deletion mutants) for heterologous...
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An Optimized Growth Medium for Increased Recombinant Protein Secretion Titer via the Type III&nbThe document provides detailed, practical methods (growth medium optimization, strain enhancements) and results focused on maximizing recombinant protein titer and secretion in a bacterial platform...
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An Overview of the Various Appropriate Types of Cell Lines for the Production of Monoclonal AntibodiJournal of Pharmaceutical Investigation
The document compares expression platforms (bacterial cells, yeast cells) relevant to the research goal, specifically focusing on key comparative metrics listed in the goal, such as efficiency...
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An effective combination of codon optimization, gene dosage, and process optimization for high-levelThe document, while focusing on a non-HMP protein (fibrinolytic enzyme), uses one of the key expression platforms mentioned in the goal (Komagataella phaffii/Pichia pastoris). Crucially, it provides...
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An examination of the endoplasmic reticulum stress response in the filamentous fungus Aspergillus niThe document is a PhD thesis focusing on improving recombinant protein production (RPP) yields by mitigating the Endoplasmic Reticulum Stress Response (ERSR) bottleneck in the filamentous fungus,...
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An influenza HA stalk reactive polymeric IgA antibody exhibits anti-viral function regulated by binaThe document provides a detailed functional characterization of polymeric IgA (sIgA), which is one of the target human milk proteins. It specifically examines how polymerization and IgA Fc...
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Antigen Delivery System IIThe document provides a detailed comparative analysis of various bacterial expression platforms (Salmonella, Lactobacillus, E. coli, etc.), focusing on vector components, secretion mechanisms...
2 claims
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An inverse metabolic engineering approach for the design of an improved host platform for over-expreThe document details an inverse metabolic engineering strategy applied to Escherichia coli (a target platform) to significantly improve the specific yield/titer of recombinant proteins by identifying...
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An optimized growth medium for increased recombinant protein secretion titer via the type III secret2021
The document details experimental methods for optimizing growth medium components (salts, buffers, carbon sources) to significantly increase recombinant protein secretion titer in a bacterial...
3 claims
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Antibody Peptide Based Antifungal Immunotherapy2012
The document discusses the production of recombinant bioactive proteins (antifungal peptides/antibodies, including scFv) using yeast expression systems, specifically mentioning Wickerhamomyces...
2 claims
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Anti-Inflammatory and Immune-Modulatory Properties of Milk Lactoferrin2023
The research goal involves producing bioactive human milk proteins, including lactoferrin. This document focuses entirely on defining the specific anti-inflammatory and immune-modulatory...
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Anti-infectivity of camel polyclonal antibodies against hepatitis C virus in Huh7.5 hepatomaThe document investigates the bioactivity (anti-infectivity) of several target human milk proteins mentioned in the research goal (lactoferrin, alpha-lactalbumin, casein, IgGs), using naturally...
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Antibacterial Activity of Recombinant Pig Intestinal Parasite Cecropin P4 Peptide Secreted from &Anim. Sci.
Vol.
The document provides detailed strain construction data (pPICZB vector, AOX1 promoter, S. cerevisiae alpha-factor secretion signal) and optimization results (titer/induction) for a bioactive...
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Antibacterial properties of lactoferrin A bibliometric analysis from 2000 to early 20222022 · Frontiers in Microbiology
The document is a bibliometric review focused on Lactoferrin (a target human milk protein) and its bioactivity (antibacterial properties). The analysis explicitly identifies 'expression' and...
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Antibody engineering by combining genome editing, deep sequencing, and deep learningThe document focuses on engineering therapeutic antibodies, but it addresses a core challenge of the research goal: PTM fidelity. The thesis explicitly discusses the drawbacks of microbial hosts...
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Antibody manufacture in transgenic animals and comparisons with other systemsThe document compares various recombinant protein expression platforms (including bacteria and yeast) based on post-translational modifications (PTMs), specifically glycosylation fidelity, and how...
3 claims
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Antifoam addition to shake flask cultures of recombinant Pichia pastoris increases yieldThe document investigates optimization strategies (effect of antifoaming agents) for the Pichia pastoris expression platform, which is one of the platforms listed in the research goal. The findings...
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Antifungal Activity of Antimicrobial Peptides and Proteins against Aspergillus fumigatus2020
The document investigates the bioactivity (antifungal activity) of two human milk proteins central to the research goal: lysozyme and lactoferrin. Validation of bioactivity is a critical required...
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Antifungal Peptides as Therapeutic Agents2020 · Frontiers in Cellular
The document reviews the production of bioactive peptides (Antifungal Peptides, AFPs), explicitly mentioning 'natural, heterologous and synthetic production' using industrial microorganisms,...
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Antifungal peptides from living organismsFrontiers in Microbiology
The research goal is centered on comparing different microbial expression platforms (Pichia, Saccharomyces, bacteria, fungi) based on production metrics (titer, secretion, PTM fidelity). This...
3 claims
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Antigenic validation of recombinant hemagglutinin-neuraminidase protein of Newcastle disease virus eThe document directly compares two key expression platforms (*Saccharomyces cerevisiae* and *Pichia pastoris*) mentioned in the goal, utilizing detailed methods like codon usage analysis. Crucially,...
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Antimicrobial Activity of Lactoferrin-Related Peptides and Applications in Human and Veterinary Medi2016 · Veterinary Sciences
The document reviews the antimicrobial bioactivity and clinical applications of a primary target protein (Lactoferrin) and related proteins (alpha-lactalbumin), specifically discussing their role and...
2 claims
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Antimicrobial Peptides, Their Production, and Potential in the Fight Against Antibiotic-Resistant Pa2025
The document reviews the production and function of Antimicrobial Peptides (AMPs). It explicitly identifies milk lactoferrin as a potent antimicrobial agent. Lactoferrin is one of the key bioactive...
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Antimicrobial Peptides A New Hope in Biomedical and Pharmaceutical FieldsThe document reviews Antimicrobial Peptides (AMPs) and explicitly mentions lactoferrin (a core target protein in the research goal) as an animal-originated AMP identified in cow milk. The discussion...
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Antimicrobial Peptides Mechanisms, Applications, and Therapeutic PotentialThe research goal focuses on bioactive human milk proteins (HMPs), notably lactoferrin and lysozyme, which are well-known antimicrobial proteins or precursors to antimicrobial peptides (AMPs). This...
3 claims
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Antimicrobial Peptides Multifunctional Drugs for Different Applications2012
The document reviews Antimicrobial Peptides (APs) and explicitly identifies lactoferrin, a key target Human Milk Protein (HMP) in the research goal, as a related peptide. While the review focuses on...
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Antimicrobial Peptides New Frontiers in the Therapy of InfectionsThe document focuses on Antimicrobial Peptides (AMPs) and Host Defense Peptides (HDPs). This class includes key target proteins of the research goal (lactoferrin, lysozyme). The text discusses the...
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Antimicrobial Peptides The Production of Novel Peptide-Based Therapeutics in Plant Systems2023
The document primarily focuses on Antimicrobial Peptide (AMP) production in plant systems, which is outside the main platforms (fungi, bacteria) specified in the research goal. However, it explicitly...
3 claims
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Antimicrobial Peptides towards Clinical Application—A Long History to Be Concluded2024 · Concluded. Int.
The document reviews Antimicrobial Peptides (AMPs), a category that includes key target proteins (lactoferrin and lysozyme) listed in the research goal. While the review focuses on clinical...
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Antimicrobial Proteins for Human HealthAlthough the target protein is Ovocalyxin-32 (egg) rather than a human milk protein, the document directly addresses the research goal's focus on expression platform comparison and engineering...
2 claims
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Antimicrobial activity of recombinant E. coli expressed chicken AvBD-2 and its mRNA expression in InThe document details the cloning, expression, purification, and bioactivity assessment of a recombinant protein using the E. coli bacterial expression platform, which is one of the target platforms...
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Antimicrobial and Prebiotic Activity of Lactoferrin in the Female Reproductive Tract A Comprehensive2021
The research goal requires comparing the bioactivity of recombinantly expressed human milk proteins (including lactoferrin) across different platforms. This document provides a comprehensive review...
3 claims
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Antiparasitic activity of the iron-containing milk protein lactoferrin and its potential derivatives2024 · Frontiers in Parasitology
The document provides detailed information on the bioactivity (antiparasitic activity and iron chelation) of one of the key target human milk proteins (lactoferrin). This bioactivity data is...
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Antiviral activity of mink interferon alpha expressed in the yeast Pichia pastoris2022 · Frontiers in Veterinary Science
Although the target protein is mink interferon, the document provides detailed, transferable data concerning one of the key specified expression platforms, Pichia pastoris. It addresses critical...
3 claims
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Apoptosis-Like Death in Bacteria Induced by HAMLET, a Human Milk Lipid-Protein Complex2011 · PLOS ONE
The document investigates the bioactivity (bactericidal activity) of one of the target proteins, alpha-lactalbumin (ALA), when complexed with oleic acid (HAMLET). It specifically notes that the...
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Applicability of the constitutive GAP promoter for recombinant human insulin hormone production in P2024 · Journal of Engineering and Natural Sciences
The document provides highly specific and transferable data crucial for the research goal's comparative analysis, focusing on one of the target expression platforms (*Pichia pastoris*). It details...
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Application of nuclear magnetic resonance spectroscopy for the evaluation of folding variability inAlthough the document focuses on S100 calcium-binding proteins and food allergies, it provides highly detailed methodological data on high-throughput cloning, expression screening (solubility and...
3 claims
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Application of simple fed-batch technique to high-level secretory production of insulin precursor usThe document investigates high-level secretory production, focusing heavily on titer and secretion optimization using Pichia pastoris, which is one of the target expression platforms. It provides...
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Applications of Site-Specific Labeling to Study HAMLET, a Tumoricidal Complex of α-Lactalbumin and O2011 · PLOS ONE
The document investigates alpha-lactalbumin, a key human milk protein, produced recombinantly in E. coli (a target platform). It details protein engineering (C-terminal extension) and enzymatic...
3 claims
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Applications of recombinant Pichia pastoris in the healthcare industryThe document is a detailed review focusing on Pichia pastoris (a key platform in the research goal) for recombinant protein production, specifically addressing critical comparison metrics such as...
3 claims
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Aspergillus as a multi-purpose cell factory current status and perspectives2010
The document reviews Aspergillus, a filamentous fungus and a specific expression platform explicitly mentioned in the research goal. It details advances in molecular tools, genetic engineering,...
3 claims
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Assessment of E. coli Expression System for Overexpression of Active Recombinant Ocriplasmin2020
The document compares the performance (titer/yield and bioactivity) of two expression platforms crucial to the research goal: E. coli (bacteria) and Pichia pastoris (yeast). While the specific...
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Assessment of Escherichia coli-derived Recombinant Human Bone Morphogenic Protein-2 on Fertilization2023
The document investigates the safety and bioactivity of a recombinant protein produced in a relevant expression platform (E. coli, a bacterium). Crucially, it discusses the structural difference...
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Assessment of recombinant protein production in E. coli with Time-Gated Surface Enhanced Raman Spect2020
The document investigates a sophisticated analytical methodology (TG-SERS) for assessing recombinant protein production quality, specifically protein folding, secondary structure, and aggregation, in...
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Assessment of a novel high-throughput process development platform for biopharmaceutical protein proProf. Dr.
The document focuses on developing and assessing a novel high-throughput process development platform for recombinant protein production. While the specific expression platform is plants (not the...
3 claims
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Automatisiertes Hochdurchsatzscreening zur Optimierung und Vorhersage der rekombinanten ProteinexpreProf. Dr.
The document, a PhD thesis, details robotic high-throughput screening and optimization methods for recombinant protein expression in Escherichia coli (a targeted expression platform). It focuses on...
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BI-VARIATE GROWTH MODEL OF PICHIA PASTORIS INCLUDING OXYGEN CONSIDERATIONS AND ITS IMPORTANCE IN RECThe document focuses on optimizing the bioprocess kinetics (growth models, oxygen, methanol, feeding strategies) of Pichia pastoris. Pichia is a key expression platform specified in the research...
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BI-VARIATE GROWTH MODEL OF PICHIA PASTORIS INCLUDING OXYGEN CONSIDERATIONS AND ITS IMPORTANCE IN RECThe document focuses on developing bi-variate growth models and optimizing feeding methods (kinetics, dissolved oxygen, methanol concentration) for Pichia pastoris. Pichia is a core expression...
3 claims
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Bacillus megaterium Versatile Tools for Production, Secretion and Purification of Recombinant Protei2006
The document focuses on a key expression platform listed in the research goal (bacteria, specifically Bacillus megaterium). It details the development and application of versatile tools for...
3 claims
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Bacterial expression of self-assembling peptide hydrogelators2014
The document is a PhD thesis detailing the construction, cloning, expression, and purification strategies for recombinant peptides/proteins using a bacterial expression platform (Chapter 3). The...
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Benefits of Lactoferrin, Osteopontin and Milk Fat Globule Membranes for Infants2017
The document is a review focused entirely on the target bioactive human milk proteins (lactoferrin, alpha-lactalbumin, lysozyme, sIgA, caseins) and their function/bioactivity in infant nutrition....
3 claims
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Benzylisoquinoline Alkaloid Biosynthesis in Sacred Lotus (Nelumbo nucifera)The document, a PhD thesis, details the methodology and outcomes of engineering yeast strains (a platform specified in the research goal, e.g., Saccharomyces) to express complex foreign enzymes...
3 claims
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Beyond Purification Evolving Roles of Fusion Tags in Biotechnology2025 · Biotechnology. Curr.
The document reviews the use of fusion tags (e.g., SUMO, MBP, Trx, GFP) to enhance key production metrics relevant to the goal: soluble expression, folding efficiency, stability, and purification....
2 claims
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Biocatalytic production of chitosan polymers from shrimp shells, using a recombinant enzyme produced2012 · Journal of Molecular Biology
While the target protein is Chitin deacetylase and not a human milk protein, the document provides practical, non-theoretical data on strain construction (cloning the S. cerevisiae gene) and...
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Biochemical properties and yields of diverse bacterial laccase-like multicopper oxidases expressed iThe document investigates expression optimization (titer/yield, effect of signal peptides on secretion/solubility, and functional processing via metal co-factor insertion) for recombinant proteins in...
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Biochemische und molekularbiologische Charakterisierung neuartiger Oxidoreduktasen aus BasidiomyceteProf. Dr.
The document details specific outcomes of heterologous protein expression (including solubility and activity challenges) in two key expression platforms targeted by the research goal: the yeast...
3 claims
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Bioengineered silkworm model for expressing human neurotrophin-4 with potential biomedical applicatiAlthough the primary focus is neurotrophin-4 expression in silkworm, the document details complex protein engineering strategies (codon optimization, specific promoters/signal peptides, titer...
3 claims
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Bioinformatic Analysis of the Human Recombinant Iduronate 2-Sulfate SulfataseAlthough the target protein (Iduronate 2-Sulfate Sulfatase) is not a human milk protein, the document directly addresses core engineering challenges relevant to the goal. It discusses optimizing...
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Bioinformatics And Epidemiological Evidence Link Yeast Protein Containing Hpv And Hepatitis B VaccinThe document discusses significant regulatory and safety issues (autoimmunity risk due to host cell protein contamination/molecular mimicry) associated with using the Saccharomyces cerevisiae...
3 claims
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Biologically Inspired Engineering for Protein StabilisationThe document uses lysozyme (a target human milk protein) as a model protein. It also involves cloning and optimizing recombinant protein expression in bacteria (one of the target expression...
2 claims
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Biomedical and Nutritional Applications of Lactoferrin2023 · Journal of Peptide Research and Therapeutics
The document is a review focused entirely on Lactoferrin (a key target protein), detailing its structure, post-translational modifications (specifically glycosylation), stability, and various...
3 claims
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Biopharming of Lactoferrin Current Strategies and Future Prospects2025
The document is a comprehensive review on the biopharming and production strategies for Lactoferrin, a key target human milk protein. It explicitly discusses and compares multiple specified...
3 claims
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Biophysical characterization of folding and aggregation behavior in model single- and multi-domain pAlthough the study focuses on model proteins (scFv, Chymotrypsinogen A) rather than human milk proteins, the core research involves the biophysical characterization (folding, stability, aggregation)...
2 claims
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Bioprocess development for recombinant protein production in Pichia pastoris at different scalesIng. Dr.
The document focuses on optimizing bioprocess development, high-throughput screening, media composition (defined mineral media), and operational conditions (fed-batch, auto-induction) specifically...
3 claims
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Bioprocess optimisation for high cell density endoinulinase production from recombinant AspergillusThe document focuses on detailed bioprocess optimization and strain engineering (promoters, secretion signals) for achieving high titer and secretion of a recombinant protein in a filamentous fungi...
3 claims
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Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein prod2021
The document focuses on characterizing and optimizing novel methanol-independent promoters (PPDF, PUPP) and bioprocess performance (titer/qp, fed-batch) in Pichia pastoris. Pichia is a core...
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Biosensor-guided rapid screening for improved recombinant protein secretion in Pichia pastoris2023
The document investigates methods (a split-GFP biosensor) to improve recombinant protein secretion and identify high-titer clones in Pichia pastoris. Pichia is one of the required expression...
2 claims
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Biosynthese des Taxols Klonierung und Expression des Taxa-4(5),11(12)-dien-Synthase-Gens aus Taxus bProf. Dr.
The document, a PhD thesis, provides detailed, practical strain engineering and expression data for expressing a complex heterologous enzyme (Taxadiene synthase) in Escherichia coli (a target...
3 claims
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Biosynthese von Carotinoiden mittels rekombinanten MikroorganismenThe document details strain engineering, optimization (titer), analytical methods (HPLC/MS), and transcriptional analysis (microarrays/qPCR) for heterologous biosynthesis in bacterial expression...
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Biotechnological Insights on the Expression and Production of Antimicrobial Peptides in Plants2021
The document primarily focuses on plant expression systems for antimicrobial peptides (AMPs), whereas the research goal specifies microbial systems for human milk proteins (HMPs). However, both...
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Biotechnologische Darstellung und strukturelle Charakterisierung fucosylierter Oligosaccharide2013 · Prof. Dr.
The document details the metabolic engineering and production optimization (titer, fed-batch) of complex Human Milk Oligosaccharides (HMOs) using the bacterial expression platform, E. coli. While the...
3 claims
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Bovine Colostrum in Pediatric Nutrition and Health2024
The document provides critical contextual information regarding the importance of target human milk proteins (lactoferrin, caseins, glycans) in pediatric nutrition, justifying the need for PTM...
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Bovine Lactoferrin Enhances Proliferation of Human Peripheral Blood Lymphocytes and Induces CytokineThe research goal requires evaluation of bioactivity and PTM fidelity for human milk proteins (lactoferrin). This document provides detailed methods (MTT, ELISA) and results for assessing the...
3 claims
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Bovine lactoferrin free of lipopolysaccharide can induce a proinflammatory response of macrophagesThe research goal requires assessing the bioactivity of recombinant human lactoferrin (LF). This document details the specific immunomodulatory bioactivity mechanisms (LPS-free LF activating...
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Breast Milk A Source of Functional Compounds with Potential Application in Nutrition and Therapy2021
This review covers functional compounds in breast milk, including the target human milk proteins (HMPs), and explicitly addresses the difficulty of isolating and producing them on scale. Crucially,...
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Burden Imposed by Heterologous Protein Production in Two Major Industrial Yeast Cell Factories Ident2022 · Frontiers in Fungal Biology
The document reviews strategies for mitigating 'burden' and improving heterologous protein titer and production efficiency in two specific hosts central to the research goal: Saccharomyces cerevisiae...
3 claims
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CD4+ T cell mediated chronic intestinal disease immune regulation versus inflammationProf. Dr.
Although the main topic is mucosal immunity and IBD, the document (Chapter III) details the use and engineering of a bacterial expression platform (E. coli NISSLE 1917) utilizing the AIDA...
2 claims
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CLONAGEM, EXPRESSÃO E CARACTERIZAÇÃO DA NUCLEOPROTEÍNA RECOMBINANTE DO VÍRUS DA BRONQUITE INFECCIOSAArq. Inst. Biol.
The document provides a direct comparative analysis of protein expression yield (titer) between two key platforms listed in the research goal: Escherichia coli (bacteria) and Pichia pastoris. It...
3 claims
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CP40 from Corynebacterium pseudotuberculosis is an endo-β-N-acetylglucosaminidaseThe document characterizes a novel bacterial endoglycosidase (EndoS-like enzyme) that acts specifically on N-glycans. Analyzing and ensuring the fidelity of Post-Translational Modifications (PTMs),...
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CRISPR Cas9-mediated targeted knock-in of large constructs using nocodazole and RNase HII.2023
The research goal requires 'Detailed strain construction data' and 'strain engineering outcomes.' This document provides methods for optimizing the targeted knock-in of large constructs (5.3–7.1 kb...
2 claims
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CRISPR Cas9-vermittelter Knockout von sechs pflanzenspezifischen Glykosyltransferase-Genen in NicotiProf. Dr.
Although the platform is plants (Nicotiana benthamiana) rather than microbes, the document is highly relevant to the core research metric of PTM fidelity and bioactivity. It details sophisticated...
3 claims
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CamOptimus a tool for exploiting complex adaptive evolution to optimize experiments and processes inThe document, while focused on a software tool (CamOptimus), uses a case study that directly addresses the research goal. The case study involves optimizing culture conditions (a crucial factor in...
2 claims
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Can microbes compete with cows for sustainable protein production - A feasibility study on high qualThe document investigates the theoretical production feasibility (modeling titer and yield/productivity) of a target human milk protein (Alpha Lactalbumin, using bovine alpha-lactalbumin as a case...
2 claims
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Carbon source dependent promoters in yeastsThe research goal requires detailed strain construction data (promoters, signal peptides) for yeast platforms (Pichia, Saccharomyces). This review directly analyzes the function and application of...
3 claims
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Casamino acids facilitate the secretion of recombinant dengue virus serotype-3 envelope domain III iThe document utilizes Pichia pastoris, one of the target expression platforms, and focuses on optimizing culture conditions (Casamino Acids, temperature) to maximize secretion titer (a primary...
2 claims
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Casein phosphopeptides drastically increase the secretion of extracellular proteins in Aspergillus aThe document investigates bottlenecks in the secretory pathway of filamentous fungi (Aspergillus awamori), which is one of the key expression platforms listed in the research goal. It reports a...
3 claims
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Cattle Mammary Bioreactor Generated by a Novel Procedure of Transgenic Cloning for Large-Scale Produ2008 · PLOS ONE
The document investigates the production of a core target protein (human lactoferrin) and provides high-level benchmark data (titer, glycosylation/PTM fidelity, and bioactivity) using a highly...
3 claims
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Cell-Free Glycoengineering of the Recombinant SARS-CoV-2 Spike Glycoprotein2021 · Frontiers in Bioengineering
Although the target protein is SARS-CoV-2 Spike rather than a human milk protein, the document directly addresses the critical issue of PTM fidelity (glycosylation) for recombinant proteins expressed...
3 claims
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Cell-free protein synthesis as a novel tool for directed glycoengineering of active erythropoietin2018
The document investigates directed glycoengineering using eukaryotic cell-free synthesis (insect cell lysate) to achieve defined PTM fidelity, a core technical challenge in the research goal...
2 claims
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Cell Engineering and Molecular Pharming for BiopharmaceuticalsThe document reviews and compares microbial host systems (bacteria, yeast/fungi implicitly) used for recombinant protein production (molecular pharming). It specifically addresses critical comparison...
3 claims
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Cell Membrane-Interrupting Antimicrobial Peptides from Isatis indigotica Fortune Isolated by a Bacil2019
The document focuses on using a bacterial expression platform (*Bacillus subtilis*) for protein production, which is one of the key expression platforms listed in the research goal ('bacteria'). It...
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Cellobiohydrolase B of i Aspergillus niger i over-expressed in i Pichia pastoris i stimulates hydrolThe document investigates the expression of a recombinant protein in Pichia pastoris, one of the target expression platforms. Crucially, it provides specific data regarding Post-Translational...
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Cellular Uptake and Release of Intact Lactoferrin and Its Derivatives in an Intestinal Enterocyte Mo2019 · Biol. Pharm. Bull.
The document investigates the bioactivity (intestinal uptake and release) of recombinant human lactoferrin (a target protein). The recombinant hLF tested was produced in Aspergillus niger, which...
3 claims
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Cellular responses to the induction of recombinant genes in Escherichia coli fed-batch cultures1999 · Prof. Dr.
The document investigates detailed physiological and engineering aspects (titer, plasmid stability, growth inhibition, stress responses, fed-batch kinetics, scale-up) specific to the Escherichia coli...
3 claims
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Ceruloplasmin Macromolecular Assemblies with Iron-Containing Acute Phase Proteins2013 · PLOS ONE
The document provides detailed structural analysis and functional characterization (bioactivity and specific protein-protein interactions) of Lactoferrin (Lf), which is one of the key Human Milk...
3 claims
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Challenges Associated With the Formation of Recombinant Protein Inclusion Bodies in Escherichia coli2021 · Frontiers in Bioengineering
The document focuses on optimizing recombinant protein production in E. coli (a target platform) by addressing critical factors explicitly mentioned in the research goal, such as improving...
2 claims
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Challenges and Opportunities for the Large-Scale Chemoenzymatic Glycoengineering of Therapeutic N-GlThe research goal focuses heavily on PTM fidelity (glycosylation) in specific microbial expression platforms (Pichia, Saccharomyces). This document provides a detailed review of challenges and...
2 claims
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Challenges and Opportunities in the Process Development of Chimeric Vaccines2023
The document reviews challenges and opportunities in bioprocess development, including high-throughput tools, QbD, PAT, and optimization strategies for recombinant proteins expressed in key platforms...
3 claims
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Challenges in biocatalysis - case studies on protein expression, engineering and application2012 · Prof. Dr.
This PhD thesis contains detailed case studies on protein expression and engineering in key platforms mentioned in the goal. Specifically, it addresses 'Optimized expression of the dirigent protein...
3 claims
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Chances and drawbacks of derepressed recombinant enzyme production in continuous cultivations with KThe document investigates expression platform performance in Komagataella phaffii (a Pichia yeast listed in the research goal). It provides detailed, practical data on critical factors relevant to the...
3 claims
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Characterisation of a recombinant phosphorylcholine-free form of the immunomodulatory filarial nemat2017
The PhD thesis details the use of the Pichia pastoris expression platform for producing a recombinant secreted protein (ES-62). It includes explicit mention of strain construction (sub-cloning,...
3 claims
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Characterisation of a recombinant β-xylosidase (xylA) from Aspergillus oryzae expressed in Pichia paThe document does not focus on human milk proteins, but it provides detailed, transferable methodological data regarding heterologous expression in Pichia pastoris (a target platform) using specific...
2 claims
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Characterization and heterologous expression of cis-abienol synthase from balsam fir (Abies balsameaThe document provides detailed outcomes, strain comparisons, and titer data (12.8 mg/L) regarding the heterologous expression and metabolic engineering of a complex enzyme in one of the primary...
3 claims
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Characterization of Bioactive Recombinant Human Lysozyme Expressed in Milk of Cloned Transgenic Catt2011 · PLOS ONE
The document focuses on characterizing one of the target human milk proteins (lysozyme) and measures key metrics crucial for the research goal (titer/expression level and bioactivity/physicochemical...
3 claims
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Characterization of Escherichia coli as a Recombinant Protein Expression Host and its OptimizationJournal of Science and Technology
The research goal requires comparing bacterial expression platforms (*E. coli* is the primary example) regarding titer, secretion, and PTM fidelity (glycosylation). This document provides a detailed...
2 claims
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Characterization of Full-Length and Truncated Recombinant κ-Carrageenase Expressed in Pichia pastori2017 · Frontiers in Microbiology
The document focuses on optimizing the expression of a recombinant protein using one of the required platforms (*Pichia pastoris*). It provides highly relevant, transferable data concerning strain...
3 claims
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Characterization of Recombinant Antimicrobial Peptide BMGlv2 Heterologously Expressed in Trichoderma2022 · Mol. Sci.
The document investigates the heterologous expression, yield, and bioactivity characterization of a recombinant protein in a target expression platform (*Trichoderma reesei*, a filamentous fungus)....
3 claims
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Characterization of a Novel Aspartic Protease from Rhizomucor miehei Expressed in Aspergillus niger2021
The document investigates heterologous protein expression (an aspartic protease) in Aspergillus niger, which is a required 'filamentous fungi' platform. It provides detailed data on strain...
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Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for r2016
The document characterizes a novel episomal vector system for Pichia pastoris, one of the key expression platforms in the research goal. The findings directly relate to improving recombinant protein...
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Characterization of functional human insulin produced using the Pichia pastoris expression systemJournal of Applied Pharmaceutical Science Vol
Although the target protein is Human Insulin (not Human Milk Proteins), the document uses and compares the required expression platforms (Pichia, Saccharomyces, bacteria) and provides highly...
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Characterization of elements of gene expression and production of virus-like particles in the moss PProf. Dr.
Although the platform is moss (*Physcomitrium patens*) and the product is virus-like particles (VLP), the dissertation focuses extensively on the core engineering elements required for heterologous...
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Characterization of recombinant human lactoferrin N-glycans expressed in the milk of transgenic cows2017 · PLOS ONE
The research goal focuses heavily on PTM fidelity (glycosylation) of human milk proteins like lactoferrin. This document provides critical reference data by characterizing the N-glycan structures of...
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Characterization of recombinant human lysosomal beta-hexosaminidases produced in the methylotrophicUniv. Sci.
The document focuses on producing and characterizing a recombinant human protein (beta-hexosaminidase) in one of the required expression platforms (*Pichia pastoris*). It provides specific,...
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Characterization of the Recombinant Exopeptidases PepX and PepN from Lactobacillus helveticus ATCC 12013 · PLOS ONE
The document details high-titer production strategies (titer/secretion metrics) for recombinant proteins using E. coli BL21(DE3), which is one of the target expression platforms (bacteria)....
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Characterization of the molecular features of Oncostatin M determining receptor activationProf. Dr. Dr.
The document, while focusing on Oncostatin M (not a target HMP), utilizes a relevant expression platform (prokaryotic system/bacteria, 3.2.17) and includes detailed methods for recombinant protein...
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Characterization of the secretion and anchoring domains of Caulobacter crescentus SapA metalloproteaThe document investigates a bacterial expression platform (*Caulobacter crescentus* Type I secretion system), providing detailed strain engineering outcomes, identification of C-terminal signal...
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Charakterisierung der Glykosylierungsstelle von Glykoproteinen mittels Matrix- unterstützter LaserdeAlthough the proteins (A1AT, GM-CSF) and expression systems (HEK293, NZL) analyzed do not match the specific targets (HMPs in Pichia/Saccharomyces), the document is a detailed PhD thesis focused...
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Charakterisierung rekombinant produzierter Virus-ähnlicher Partikel des humanen Parvovirus B19Prof. Dr.
The document, a PhD thesis, provides detailed, practical information on establishing and optimizing an expression platform (*Saccharomyces cerevisiae*) which is one of the key platforms specified in...
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Cheese-whey permeate improves the fitness of Escherichia coli cells during recombinant protein produ2023
The document investigates using cheese-whey permeate as a low-cost inducer to optimize recombinant protein production titer and cell fitness in E. coli, a key bacterial expression platform listed in...
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Chlamydomonas reinhardtii A Factory of Nutraceutical and Food Supplements for Human Health2023
The document discusses Chlamydomonas reinhardtii as an expression platform for functional proteins and nutraceuticals, highlighting aspects crucial to the research goal, such as genetic engineering...
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Cholera Toxin B One Subunit with Many Pharmaceutical Applications2015
The document, while focusing on Cholera Toxin B (CTB), explicitly states it will 'summarize various recombinant expression systems available for recombinant CTB bioproduction.' Since the research...
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Cloning, expression in Pichia pastoris, and characterization of a thermostable GH5 mannan endo-1,4-βThe document focuses on heterologous expression, secretion, titer determination (243 μg/mL), and post-translational modification (glycosylation confirmed) in Pichia pastoris. Pichia is one of the key...
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Cloning, periplasmic expression and purification of hGM-CSF (Human Granulocyte - Macrophage ColonyStAlthough the target protein (hGM-CSF) is not a human milk protein, the document details strain construction, vector usage (pET-22b), periplasmic secretion using the pelB signal peptide, and...
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Cloning, sequence analysis, expression of Cyathus bulleri laccase in Pichia pastoris and characterizThe document provides detailed, high-quality data directly relevant to several comparison criteria required by the research goal, specifically for the Pichia pastoris platform. It details the use of...
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Cloning And Extracellular Expression Of Recombinant Tissue Plasminogen Activator (Rt-Pa) Using A MetThe document provides detailed strain construction data (pICZαA vector, alpha-factor secretion signal, Mut+ strain), secretion outcomes, and addresses PTM concerns (glycosylation/avoidance of...
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Cloning And Extracellular Expression Of Recombinant Tissue Plasminogen Activator (Rt-Pa) Using A MetAlthough the target protein is tPA (not an HMP), the document provides highly specific and transferable engineering data required by the research goal's notes. It details strain construction (Pichia...
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Cloning Of A Β-Glucosidase Gene (Bgl1) From Traditional Starter Yeast Saccharomycopsis Fibuligera BmAlthough the document focuses on expressing a non-HMP (beta-glucosidase), it provides concrete, transferable data on expression platform comparisons (Pichia pastoris vs. Saccharomyces cerevisiae)...
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Cloning and Expression of Camel Pro-Chymosin Encoding Gene in E. coli and Characterization of the ObThe document provides specific data on recombinant protein expression in a target bacterial platform (E. coli), including strain details (BL21(DE3), tet-promoter), quantitative titer (260 mg/L), and...
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Cloning and Expression of Recombinant Human Growth Hormone in E. coli High-Yield Production and BiolJournal of Medical Microbiology
Although the target protein is Human Growth Hormone (HGH) and not a human milk protein, the document directly addresses one of the required expression platforms (Bacteria/E. coli) and provides...
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Cloning and Expression of Phytase appA Gene from Shigella sp. CD2 in Pichia pastoris and Comparison2016 · PLOS ONE
The study directly compares two key expression platforms specified in the research goal (Pichia pastoris and E. coli/bacteria). It provides specific data on the influence of the host system on...
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Cloning and Expression of an Active Aspartic Proteinase Gene From Aspergillus Oryzae DRDFS13 in PichThe document directly addresses the performance characteristics (titer, secretion, PTM discussion, vector/promoter details like pGAPZαA and GAP promoter) of one of the key target expression...
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Cloning and Expression of the Human Extracellular Superoxide Dismutase (EC-SOD) Gene in Probiotic LaJournal of Bioscience
The document provides detailed strain construction data (use of signal peptide for secretion) and engineering outcomes (protein purification, multimerization, and confirmation of bioactivity) for a...
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Cloning and Extracellular Expression of Recombinant Tissue Plasminogen Activator (RT-PA) Using a MetThe document provides detailed strain construction data, vector systems (pICZαA), promoter usage (AOX1), and secretion strategies (alpha-factor signal peptide) for the Pichia pastoris expression...
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Cloning and Extracellular Expression of Recombinant Tissue Plasminogen Activator (RT-PA) Using a MetAlthough the target protein is tPA (not a human milk protein), the document provides highly relevant methodological and performance data for one of the required expression platforms (Pichia...
3 claims
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Cloning and expression of Aspergillus flavus urate oxidase in Pichia pastorisThe document investigates heterologous protein expression in Pichia pastoris (a target platform) and compares its performance generally against Saccharomyces cerevisiae (another target platform). It...
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Cloning and expression of Saccharomyces cerevisiae SUC2 gene in yeast platform and characterization2016
The document directly addresses the comparison of two key expression platforms (Pichia pastoris and Saccharomyces cerevisiae) required by the research goal. It provides detailed technical data on...
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Cloning and expression of human granulocyte-macrophage colony stimulating factor (hGM-CSF) in PichiaAlthough the document expresses hGM-CSF (not a Human Milk Protein), it uses one of the required expression platforms (Pichia pastoris) and focuses intensely on the comparison criteria listed in the...
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Cloning and expression of recombinant human leptin in Escherichia coliThe document investigates detailed cloning and expression techniques using one of the target expression platforms (*Escherichia coli*, bacteria) mentioned in the research goal. Although the specific...
2 claims
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Cloning and expression of recombinant human superoxide dismutase 1 (hSOD1) in Bacillus subtilis 1012The document investigates a key target expression platform (Bacillus subtilis, a bacterium) and compares its performance (secretion, solubility, enzymatic activity) favorably against other target...
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Cloning and functional expression of a human lysozyme gene (hly) from human leukocytes in Pichia pasThe document describes the detailed cloning, strain construction, optimization, and expression results (titer/activity, secretion) of recombinant Human Lysozyme (a target human milk protein) in...
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Cloning and over-expression of processing a-glucosidase I gene from Saccharomyces cerevisiae (CWH41)The document details specific strain construction and expression outcomes (using constitutive PGK1 promoter/terminator cassette and specific vectors) within Saccharomyces cerevisiae, a key expression...
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Cloning of glucoamylase gene from Aspergillus niger and its expression in Pichia pastorisThe document provides specific, practical data on heterologous protein expression (titer, secretion yield: 307 mg/L, strain construction details: pGAPZα-A vector, GAP promoter, Pichia pastoris X33...
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Co-cultivation of Aspergillus nidulans Recombinant Strains Produces an Enzymatic Cocktail as Alterna2016 · Frontiers in Microbiology
The document investigates recombinant protein expression and secretion in *Aspergillus nidulans* (a filamentous fungus platform explicitly mentioned in the research goal). Although the target...
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Co-expression of recombinant human nerve growth factor with trigger factor chaperone in E. coli2018
The document focuses on engineering the E. coli expression platform (one of the required platforms) to optimize yield, solubility, and periplasmic secretion of a complex recombinant protein using...
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Codon optimisation improves the expression of Trichoderma viride sp. endochitinase in Pichia pastoriThe document provides specific, transferable strain engineering outcomes and detailed construction data (codon optimization, signal peptide usage, measured titer improvement) in one of the key target...
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Codon optimization, expression, purification, and functional characterization of recombinant human I2013
The document provides detailed, transferable technical methods and engineering outcomes (codon optimization, use of pPICZαA vector and alpha-factor signal peptide, fermentation protocols)...
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Codon optimization prevents premature polyadenylation of heterologously-expressed cellulases from te2008 · Gen. Appl. Microbiol.
The document investigates bottlenecks (low titer/secretion due to premature polyadenylation) and strain engineering solutions (detailed codon optimization, vector construction, strain selection) for...
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Codon pair optimization (CPO) a software tool for synthetic gene design based on codon pair bias to2021
The document provides specific, highly effective strain engineering methodology (Codon Pair Optimization) for improving the expression titer of recombinant proteins in one of the key expression...
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Cold atmospheric plasma treatment enhances recombinant model protein production in yeast Pichia past2023
The document investigates a novel method (Cold Atmospheric Plasma) for significantly enhancing the titer and secretion yield (84% increase) of a recombinant protein in Pichia pastoris. Pichia is one...
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Combination of Genetic Manipulation Improved <i>Saccharomycopsis fibuligera< i> α-AmylasAlthough the target protein is fungal amylase, the study directly addresses key aspects of the research goal related to optimizing expression platforms: maximizing titer and secretion in Pichia...
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Combined Strategies for Improving the Production of Recombinant Rhizopus oryzae Lipase in Pichia pas2013
The document provides highly detailed, practical optimization strategies (titer maximization, feed strategies, temperature/pH control) for high-level heterologous protein expression in Pichia...
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Comparative analysis of surface coating properties of five hydrophobins from Aspergillus nidulans an2018
The document focuses on producing fungal hydrophobins, not human milk proteins. However, it provides highly relevant engineering data for one of the target expression platforms (bacteria/E. coli),...
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Comparative functional analysis of the human macrophage chitotriosidase.2011
The research goal focuses on specific bioactive human milk proteins, including lysozyme. The document directly analyzes and compares the antifungal properties (bioactivity) of human lysozyme against...
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Mucosal ImmunologyThe document extensively discusses the biological function, location, and importance of Secreted IgA (sIgA), which is one of the key target human milk proteins listed in the research goal. Although...
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Comparative expression of recombinant phospholipase A2 in Komagataella phaffii depending on the modiThe document investigates strain engineering (signal peptide modification: native alpha-factor vs. modified mf4i) to optimize secretion and titer in Komagataella phaffii (Pichia), which is one of the...
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Comparative genomic analysis identified a mutation related to enhanced heterologous protein productiThe document investigates enhanced heterologous protein production (HLY, likely human lysozyme, a target protein) in a specific expression platform (filamentous fungus, Aspergillus oryzae). It...
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Comparison of Different Signal Peptides for the Efficient Secretion of the Sweet-Tasting Plant Prote2021
The document, while studying Brazzein (a non-target protein), provides highly relevant engineering data concerning the optimization of secretion and titer in a key platform (Pichia pastoris). The...
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Comparison of Recombinant Human Haptocorrin Expressed in Human Embryonic Kidney Cells and Native Hap2012 · PLOS ONE
The document investigates Haptocorrin, a heavily glycosylated protein present in human milk, and compares its native form against a recombinant product. Although the expression platform (HEK293) is...
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Comparison of Host Expression Systems used for Efficient Recombinant Proteins ProductionThis is a comprehensive review article comparing the efficiency, yield, PTM fidelity (especially glycosylation), and regulatory considerations of several key expression platforms (bacteria, S....
3 claims
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Comparison of the expression in Saccharomyces cerevisiae of endoglucanase II from Trichoderma reesei2012
Although the document focuses on endoglucanases instead of human milk proteins, it directly addresses critical engineering variables relevant to the research goal within one of the required...
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Complete sucrose hydrolysis by heat-killed recombinant Pichia pastoris cells entrapped in calcium alThe document provides highly transferable engineering data relevant to optimizing protein expression in a key platform (Pichia pastoris). It specifically details methods and results regarding yield...
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Complex of human Melanotransferrin and SC57.32 Fab fragment reveals novel interdomain arrangement wi2021
The document investigates Melanotransferrin (MTf), a close structural homolog of the target protein Lactoferrin, focusing on structural features and glycosylation. Although the expression platform is...
2 claims
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Comprehensive, quantitative bioprocess productivity monitoring using fluorescence EEM spectroscopy a2014
The document details advanced spectroscopic methods (SERS, EEM, chemometrics) for quantitative bioprocess productivity monitoring and complex media quality control. These analytical tools are highly...
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Conformational Bias in 2'-Selenium-Modified Nucleosides and the Effect on Helical Structure and ExtrPart Two of the thesis is a review on 'Extracellular Recombinant Protein Production' and 'Recombinant Protein Secretion.' It covers prokaryotic and eukaryotic export mechanisms, signal recognition,...
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Construction and characterization of a chimerical cytokine which binds to cellulose fusion of a bactThe document, a PhD thesis, focuses on optimizing the expression, purification, and functional testing of a recombinant fusion protein (Steel Factor-CBD) in a bacterial expression platform (E. coli)....
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Construction of Quintuple Protease and Double Amylase Gene Deletant for Heterologous Protein ProductThe document focuses on engineering a filamentous fungus (*Aspergillus oryzae*) host strain for improved heterologous protein production efficiency (titer and secretion) by deleting protease and...
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Construction of Yeast Recombinant Expression Vector Containing Human Epidermal Growth Factor (hEGF)The document, while focusing on hEGF, directly addresses key elements of the research goal by detailing the construction of a recombinant expression vector (pPIC9) in one of the target expression...
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Construction of Recombinant Pichia pastoris Carrying a Constitutive AvBD9 Gene and Analysis of Its A2015 · Microbiol. Biotechnol.
Although the document expresses Avian beta-defensin 9 (not a Human Milk Protein), it uses a key expression platform specified in the goal (Pichia pastoris) and provides highly transferable...
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Construction of a Signal Peptide Library in Escherichia coli and Its Application in Guiding ProteinThe research goal requires detailed strain construction data, specifically regarding signal peptides, secretion, and titer optimization in bacterial platforms (E. coli). This document directly...
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Construction of a new T7 promoter compatible Escherichia coli Nissle 1917 strain for recombinant pro2020
The document provides detailed strain construction data for engineering an E. coli expression platform (bacteria, one of the target platforms) using T7 promoter systems and focuses on improving...
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Construction of a proteolysis-resistant peptide sequence of the food recombinant protein GDF-11 andThe document provides detailed strain construction data (plasmid pET-25b(+), cloning methods) and engineering outcomes (optimization for proteolysis resistance/oral bioactivity) using a bacterial...
2 claims
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Construction of model strain of yeast Saccharomyces cerevisiae with regulated expression of recombin2021 · Biol. Stud.
The document utilizes one of the target expression platforms (Saccharomyces cerevisiae) and provides detailed strain construction data, specifically focusing on controlled expression via a regulated...
3 claims
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Construction of pPIC9 Recombinant Vector Containing Human Stem Cell FactorThe document, while focusing on Stem Cell Factor (not a human milk protein), provides detailed, transferable methodological information on strain construction and vector design (pPIC9, AOX1 promoter,...
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Consuming Transgenic Goats' Milk Containing the Antimicrobial Protein Lysozyme Helps Resolve Diarrhe2013 · PLOS ONE
The document focuses on the bioactivity and clinical efficacy of human lysozyme (a key target protein) produced in a non-microbial system (transgenic goat). While the platform is incorrect,...
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Continuous Cultivation as a Tool Toward the Rational Bioprocess Development With Pichia Pastoris Cel2020 · Frontiers in Bioengineering
The document focuses on optimizing recombinant protein production (RPP) using Pichia pastoris, one of the key expression platforms listed in the research goal. It specifically addresses critical...
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Continuous secretory production in E. coli enables scalable, high-titer manufacturing of active reco2025 · Journal of Biological Engineering
The document provides practical, high-titer (1-2+ g/L) data for continuous secretory production using E. coli (a target platform). It directly addresses key research metrics (titer, secretion) and...
3 claims
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Controlling methanol feeding for recombinant protein production by Pichia pastoris under oxidation sThe document directly addresses methods for optimizing high-cell-density fermentation (titer and cell viability) in *Pichia pastoris*, which is one of the key expression platforms listed in the...
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Controlling specific growth rate for recombinant protein production by Pichia pastoris under oxidatiThe document focuses on optimizing fed-batch fermentation conditions (specific growth rate, methanol feeding, ROS control) in Pichia pastoris, which is one of the core expression platforms listed in...
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Controlling specific growth rate for recombinant protein production by Pichia pastoris under oxidatiThe document focuses on optimizing fed-batch fermentation protocols (specific growth rate, methanol feeding, high cell density) for maximizing recombinant protein titer and cell viability in Pichia...
3 claims
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Corynebacterium glutamicum A Platform For Studying Actinobacterial Protein-O-Mannosylation And High-The document investigates Corynebacterium glutamicum, a bacterial expression platform, focusing on key aspects critical to the research goal: high-yield heterologous production, protein secretion...
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Cost-Effective Protein Production in CHO Cells Following Polyethylenimine-Mediated Gene Delivery Sho2023
The document describes the production of a key target protein, human lactoferrin, using a mammalian expression system (CHO cells). Although the goal focuses on microbial systems, the titer, secretion...
2 claims
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Could protein tertiary structure influence mammary transgene expression more than tissue specific co2010
Although the primary expression platform discussed is transgenic animal mammary gland (not microbial systems listed in the goal), the document addresses core mechanistic challenges highly relevant to...
2 claims
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Cultivation strategies to enhance productivity of Pichia pastoris a review2015
The document reviews cultivation and engineering strategies (including enhancing productivity, secretion, and glycosylation/secretory pathways) specifically for Pichia pastoris, which is one of the...
3 claims
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Current Status and Perspective on the Use of Viral-Based Vectors in Eukaryotic Microalgae2022 · Microalgae. Mar.
Although the primary expression platforms listed in the goal (yeast, fungi, bacteria) do not include microalgae, this document details engineering tactics, transformation methods (viral vectors),...
2 claims
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Current advances of Pichia pastoris as cell factories for production of recombinant proteins2022 · Frontiers in Microbiology
The document is a review summarizing current advances in using Pichia pastoris (one of the key expression platforms listed in the research goal) for recombinant protein production. It specifically...
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Current breakthroughs and advances in atmospheric room temperature plasma (ARTP) technology for biom2025
The document reviews Atmospheric Room Temperature Plasma (ARTP) technology, a method used for random mutagenesis and strain improvement in microbial systems (bacteria, yeast, fungi). This technology...
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Customized yeast cell factories for biopharmaceuticals from cell engineering to process scale up2021
This review directly addresses key components of the research goal: comparing production metrics (titer, secretion, PTM fidelity) across relevant expression platforms (Saccharomyces, Pichia, K....
3 claims
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Cutinase Production in Komagataella phaffii (Pichia pastoris) Performance Differences Between Host S2025
The document investigates performance differences (titer/productivity) between specific host strains (Mut+ vs MutS) and induction regimes (methanol) in Komagataella phaffii (Pichia pastoris). Pichia...
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Cutaneous wound healing functions of novel milk-derived antimicrobial peptides, hLFT-68 and hLFT-3092025
The document investigates the specific bioactivity (antimicrobial and regenerative functions) of peptides derived from human lactoferrin, which is a core target protein in the research goal. Although...
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Cytokinetic engineering enhances the secretory production of recombinant human lysozyme in Komagatae2024
The document directly addresses the research goal by focusing on the production of a target human milk protein (lysozyme) in a target expression platform (Komagataella phaffii/Pichia). It provides...
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Cytoplasmic glycoengineering enables biosynthesis of nanoscale glycoprotein assembliesThe document details a novel cytoplasmic glycoengineering platform in bacteria (E. coli), which is one of the expression platforms specified in the research goal. The platform is designed to...
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Cytosolic Acidification Is the First Transduction Signal of Lactoferrin-Induced Regulated Cell Death2019
The document investigates the specific bioactivity mechanism (RCD induction via membrane channel interaction) of human lactoferrin (a target protein) in yeast (Candida albicans). Understanding the...
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Cytotopic (Cyto-) IL-15 as a New Immunotherapy for Prostate Cancer Recombinant Production in EscheriThe document details specific methods for optimizing recombinant protein production, purification, and recovery (addressing titer and quality/folding) from inclusion bodies in the Escherichia coli...
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Das ligninolytische System von Pleurotus sapidus Transkriptomanalyse und heterologe Expression einerProf. Dr.
The document investigates the heterologous expression of a complex protein (Aryl-alcohol oxidase) in a filamentous fungal platform (Coprinopsis cinerea) and uses Saccharomyces for vector...
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DataSheet1 Evaluation of the Anti-Inflammatory and Anti-Oxidative Effects of Therapeutic Human LactoThe document investigates a target protein (Lactoferrin) and explicitly details its production using one of the required expression platforms (bacteria: E. coli BL21(DE3) using pET28b vector)....
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Data driven approach for DSP development of recombinant glycoproteins from yeastIng. Dr.
The document directly addresses the production challenges (specifically downstream processing related to PTM fidelity, i.e., hyperglycosylation) associated with one of the key expression platforms...
3 claims
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Decolorization of Azo and Anthraquinone Dyes Using Recombinant Horseradish Peroxidase A2A Isoenzyme2025
The document details specific methods for optimizing extracellular protein production (titer and secretion) and purification using Komagataella phaffii (Pichia pastoris), one of the key expression...
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Decrease of UPR- and ERAD-related proteins in Pichia pastoris during methanol-induced secretory insuThe document investigates host physiology (UPR, ERAD, secretion efficiency) and high-titer production strategies (fed-batch, methanol induction) in Pichia pastoris. This detailed operational data and...
3 claims
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Dectin-1 Is Essential for Reverse Transcytosis of Glycosylated SIgA-Antigen Complexes by Intestinal2013
The research goal focuses on producing bioactive human milk proteins, including sIgA, and emphasizes PTM fidelity (glycosylation) and bioactivity. This document directly investigates the bioactivity...
3 claims
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Deleted in Malignant Brain Tumors-1 Protein (DMBT1) A Pattern Recognition Receptor with Multiple Bin2010 · Mol. Sci.
The document reviews the function and binding characteristics of native human mucosal defense proteins, including three key target proteins of the research goal (lactoferrin, lysozyme, and IgA). It...
3 claims
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Deletion of the GPI pre-anchor sequence in Mtf as a mechanism for generating the soluble form of theThe document, a Master's thesis, details engineering strategies (deletion of the GPI anchor sequence) to maximize the secretion and solubility of Melanotransferrin (p97). P97 is explicitly stated to...
3 claims
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Delta-Integration of Single Gene Shapes the Whole Metabolomic Short-Term Response to Ethanol of Reco2020
The document investigates the impact of a specific strain engineering strategy (multiple delta-integration of a gene under the PGK1 promoter) on metabolic burden and host stress response in...
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Deregulation of methanol metabolism reverts transcriptional limitations of recombinant i Pichia pastThe document provides detailed strain engineering strategies for improving recombinant protein titer in Pichia pastoris (a key expression platform in the research goal). Specifically, it addresses...
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Derivative of Extremophilic 50S Ribosomal Protein L35Ae as an Alternative Protein Scaffold2017 · PLOS ONE
The document investigates Alternative Binding Proteins (ABPs) that target hen egg-white lysozyme (HEL) and bovine alpha-lactalbumin (BLA), which are homologs of key Human Milk Proteins (HMPs)...
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Design, Dimerization, and Recombinant Production of MCh-AMP1–Derived Peptide in Escherichia coli and2021 · Frontiers in Fungal Biology
The document investigates recombinant protein production in E. coli (a required expression platform) detailing cloning strategies, vector usage (pET-28a), purification methods (His-tag, CNBr...
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Design and Production of a Recombinant FliC-Antigen Co-Expression Platform for Increased Vaccine EffThis PhD thesis provides detailed methodologies for the design, production, fermentation, and optimization of a recombinant protein (FliC) in a target platform specified in the goal (bacteria/E....
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Design and Synthesis of Collagen-binding Anti-microbial ProteinsThe document, a PhD thesis, provides detailed practical data and engineering outcomes on optimizing recombinant protein expression (titer, stability) using various fusion systems (Thioredoxin, SUMO,...
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Design and Unique Expression of a Novel Antibacterial Fusion Protein Cecropin B-Human Lysozyme to BeThe document describes the expression and purification methodology (including culture conditions and chromatography details) for human lysozyme—a key target protein—in a bacterial expression platform...
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Talactoferrin AlfaThe document defines Talactoferrin Alfa, which is recombinant human lactoferrin (a target protein) produced in the filamentous fungus Aspergillus niger (a target expression platform), directly...
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Design of Gene Constructs for Transgenic MaizeAlthough the document focuses entirely on transgenic maize (plants) rather than the target microbial platforms (yeast, bacteria), it provides detailed engineering principles regarding gene construct...
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Design of an efficient medium for heterologous protein production in Yarrowia lipolytica case of humThe document describes methods for optimizing chemically defined media and culture conditions (including trace metals and defined nitrogen sources) in yeast hosts (Y. lipolytica) to maximize...
3 claims
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Design of an improved universal signal peptide based on the α-factor mating secretion signal for enz2021
The document provides detailed strain construction and engineering strategies (optimized signal peptides, PTM context) specifically designed to maximize secretion yield (titer) in Saccharomyces...
3 claims
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Designing next generation recombinant protein expression platforms by modulating the cellular stressThe document focuses on detailed strain engineering in Escherichia coli (a key bacterial expression platform mentioned in the Research Goal) by modulating the cellular stress response. This directly...
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Detection of NP, N3 and N7 antibodies to avian influenza virus by indirect ELISA using yeast-expressThe document details the methods and strain construction (pESC-URA vector, GAL1/GAL10 promoters, cloning strategies) for expressing heterologous proteins in Saccharomyces cerevisiae, which is one of...
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Detoxification of pulping black liquor with Pleurotus ostreatus or recombinant Pichia pastoris follo2018
The document investigates heterologous protein production using Pichia pastoris, one of the target expression platforms, and mentions the importance of promoters and secretion signals for optimizing...
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Developing a scalable model of recombinant protein yield from Pichia pastoris the influence of cultu2009
The document provides highly relevant, transferable data regarding the optimization of one of the target expression platforms, Pichia pastoris. It focuses specifically on maximizing titer (yield) and...
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Development, Production, and Application of Recombinant Yeast Biocatalysts in Organic Synthesis2005
The document details the stable expression and engineering of glycosyltransferases in two core expression platforms specified in the research goal (Pichia pastoris and Saccharomyces cerevisiae). This...
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Development an effective system to expression recombinant protein in E. coli via comparison and opti2018
The document investigates methods (signal peptide comparison/optimization) to improve the secretion, solubility, and activity (functionality) of a heterologous protein expressed in a bacterial...
3 claims
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Development and Optimization of Novel Platforms for the Production of Recombinant ProteinsThe document directly addresses the development and optimization of one of the key expression platforms specified in the research goal: Pichia pastoris. Although the target protein studied is phytase...
3 claims
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Development and fabrication of disease resistance protein in recombinant Escherichia coli2020 · Bioresour. Bioprocess.
The document investigates the expression and optimization (codon usage via pRARE co-transformation) of a heterologous protein in the Escherichia coli expression platform, which is one of the key...
3 claims
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Development and pharmacodynamic evaluation of recombinant human lysozyme eye dropsThe document details the production, quality control (including activity assays, host protein residue detection, and specific residue checks like methanol, suggesting a Pichia expression platform),...
3 claims
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Development of Aspergillus oryzae BCC7051 as a Robust Cell Factory Towards the Transcriptional Regul2024
The document details strain engineering methods (AoPrtR disruption) in a target expression platform (filamentous fungus, Aspergillus oryzae) specifically aimed at solving the key bottleneck of...
2 claims
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Development of Glycoanalytical Methods for Characterization of Biosimilar Monoclonal AntibodiesThe research goal requires comparing Post-Translational Modification (PTM) fidelity, specifically glycosylation, across different expression platforms. This document provides detailed development and...
3 claims
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Development of New Strategy for Non-Antibiotic Therapy Bovine Lactoferrin Has a Potent Antimicrobial2013
The document investigates the antimicrobial and immunomodulator effects of bovine lactoferrin (a target protein family). This data is essential for establishing the bioactivity and functional...
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Development of New Strategy for Non-Antibiotic Therapy Dromedary Camel Lactoferrin Has a Potent Anti2013
The document focuses on lactoferrin (a key target protein) and provides critical in vitro data regarding its bioactivity (antimicrobial and immunomodulator effects). Although the protein is isolated...
2 claims
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Development of Systems for the Production of Plant-Derived Biopharmaceuticals2019
Although the focus is on plants (a platform not specifically listed in the prompt), the document provides direct comparative data necessary for the research goal. Specifically, it details the...
2 claims
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Development of a Potential Yeast-Based Vaccine Platform for Theileria parva Infection in CattleFrontiers in Immunology
Although the document focuses on producing Theileria parva antigens (not HMPs), it provides specific, practical engineering data on using Saccharomyces cerevisiae (a core expression platform in the...
3 claims
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Development of a bioprocess for recombinant protein production with a Pichia pastoris och1 knock-outProf. Dr.
The document focuses on developing an expression bioprocess and optimizing PTM fidelity (specifically addressing hypermannosylation via och1 knock-out) in the Pichia pastoris platform. Pichia is one...
3 claims
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Development of a fed-batch process for a recombinant Pichia pastoris Δoch1 strain expressing a plantThe document investigates Pichia pastoris (a target platform) and focuses heavily on optimizing PTM fidelity (glycosylation via OCH1 deletion) and improving productivity (titer) and product quality...
3 claims
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Development of a mixed feed strategy for a recombinant Pichia pastoris strain producing with a de-re2015
The document provides detailed strain engineering and bioprocess optimization data (promoter systems, mixed-feed strategy, productivity enhancement) specifically for the Pichia pastoris expression...
3 claims
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Development of a refolding and capture protocol for HRP IBs produced in E. coliAssoc. Prof. Dipl.
The document details methods (DoE, solubilization, refolding, purification) for high-yield production of a complex, disulfide-bonded enzyme (HRP) in E. coli, which is one of the platforms specified...
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Development of an AMP-SECreting Platform in E. coli for Simpler AMP Development (AMPSEC)The document investigates detailed strain engineering (using different display apparatuses and protease systems) in E. coli (a platform of interest) specifically to optimize heterologous...
2 claims
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Development of an Integrated Expression Platform for Protein Production in Eukaryotic Hosts2011
The document is a PhD thesis focusing on designing and evaluating a versatile vector system (pFlpBtM) optimized for parallel expression and rapid comparison across multiple eukaryotic expression...
3 claims
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Development of an improved Pseudoalteromonas haloplanktis TAC125 strain for recombinant protein secrThe document provides detailed strain engineering data and outcomes (gspE knockout) for a bacterial expression platform, focusing specifically on improving recombinant protein secretion efficiency...
2 claims
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Development of expression systems for the production of recombinant human Fas ligand extracellular d2018
The document is a review focused on developing expression systems (strain construction, chemical modifications, processing) for a complex glycoprotein using Pichia pastoris. Pichia is one of the key...
3 claims
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Development of gene expression systems in Bifidobacterium bifidum S17 and their application for tumoProf. Dr.
The document focuses on developing and optimizing a bacterial expression platform (*Bifidobacterium*) for heterologous protein production. Although the application is tumor therapy, the technical...
3 claims
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Development of perfusion cell culture processes for the manufacturing of therapeutic recombinant proProf. Dr.
The document details process intensification using perfusion to significantly increase volumetric productivity (titer) and control Critical Quality Attributes (CQAs), including glycosylation...
3 claims
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Development of transgenic rice lines expressing the human lactoferrin gene2010
The document expresses a target human milk protein (lactoferrin) relevant to the goal, and focuses on application in infant formula. Although the expression platform is transgenic rice (not Pichia,...
3 claims
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Development of tools and methods for genetic engineering of the recombinant protein production hostThe document is a detailed PhD thesis focusing on optimizing recombinant protein production in Pichia pastoris, a key expression platform in the research goal. It provides detailed strain...
3 claims
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Die Bedeutung intragenischer CpG Dinukleotide für die in vitro Expression von ImmunmodulatorenProf. Dr.
The document investigates strategies for optimizing gene sequences (specifically manipulating intragenic CpG content) to enhance the recombinant expression, titer, and stability of therapeutic...
3 claims
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Die Erforschung des Metabolismus von Pichia pastoris zur Verbesserung der rekombinanten ProteinproduThe document investigates metabolic engineering and strain optimization in Pichia pastoris, which is one of the key expression platforms listed in the research goal. Specifically, it addresses...
2 claims
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Dietary Lysozyme Alters Sow’s Gut Microbiota, Serum Immunity and Milk Metabolite Profile2019 · Frontiers in Microbiology
The document investigates the bioactivity (effects on gut microbiota, immunity, and milk composition) of lysozyme, which is one of the target human milk proteins (HMPs) specified in the research...
2 claims
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Different approaches to make Pichia pastoris a more versatile expression platform - process, strainProf. Dr.
The document, a PhD dissertation on optimizing Pichia pastoris as an expression platform, directly addresses several key components of the research goal. Specifically, it focuses on improving process...
3 claims
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Different results despite high homology Comparative expression of human and murine DNase1 in Pichia2025
The document investigates key engineering strategies (signal peptides, codon usage, optimization of nutrients/temperature) and expression bottlenecks (yield, folding, UPR) for expressing a human...
3 claims
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Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarrayThe document investigates differential gene expression and physiological responses (transcriptomic regulation during carbon source shift/AOX1 induction) in a core expression platform, Pichia...
3 claims
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Differential role of segments of α-mating factor secretion signal in Pichia pastoris towards granulo2020
The document investigates detailed strain engineering strategies (signal peptide modifications and codon optimization) in a core target expression platform (*Pichia pastoris*) to improve recombinant...
3 claims
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Digestive Profiles of Human Milk, Recombinant Human and Bovine Lactoferrin Comparing the Retained In2024
The document evaluates the bioactivity (digestive profile and peptide release) of recombinant human lactoferrin (a target protein) produced using Komagataella phaffii (Pichia), one of the specific...
2 claims
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Directed development of Bacillus megaterium for applications in recombinant protein production2009 · Prof. Dr.
The document is a PhD thesis focusing on the development and optimization of Bacillus megaterium, a bacterial expression platform explicitly listed in the research goal. It provides detailed strain...
3 claims
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Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of2019
The document provides detailed strain engineering strategies (disruption of vacuolar sorting components like HOPS complex genes YPT7/VAM6) and outcomes (enhanced secretion and titer confirmed in...
3 claims
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Diverse Mechanisms of Antimicrobial Activities of Lactoferrins, Lactoferricins, and Other Lactoferri2021 · Peptides. Int.
The research goal investigates the production of bioactive human milk proteins, specifically lactoferrin, focusing on PTM fidelity (glycosylation) and bioactivity. This document is a review dedicated...
3 claims
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Double disruption of the proteinase genes, tppA and pepE, increases the production level of human lyThe document details the construction of plasmids and the strain engineering strategy (promoters, Kex2 cleavage sites, protease gene disruption via pepA, pepE, etc.) for producing Human Lysozyme...
3 claims
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Double mutation of cell wall proteins CspB and PBP1a increases secretion of the antibody Fab fragmenThe document investigates detailed strain engineering (double mutation of cell wall proteins CspB and PBP1a) in a bacterial host (Corynebacterium glutamicum) specifically to improve secretion...
3 claims
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Downstream processing of recombinant human insulin and its analogues production from E. coli inclusi2021 · Bioresour. Bioprocess.
The document, while focused on insulin, compares different expression platforms (E. coli inclusion bodies vs. Pichia/Saccharomyces secretion) concerning product yield and downstream processing...
2 claims
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ERRATUM Strains and Molecular Tools for Recombinant Protein Production in Pichia pastorisThe document, an erratum for a chapter on Pichia pastoris strain engineering, discusses critical aspects directly relevant to the research goal's focus on expression platforms: improving recombinant...
2 claims
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EVALUATION OF RECOMBINANT GLUCOAMYLASE EXPRESSION BY A NATIVE AND α-MATING FACTOR SECRETION SIGNAL IThe document, while studying glucoamylase, directly addresses key technical aspects of the research goal by comparing the effectiveness of different secretion signal peptides (native vs. alpha-mating...
2 claims
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EXPERIMENTAL INVESTIGATION OF THE LIPID-BINDING MECHANISM OF OSH4 PROTEINAlthough the primary focus is the mechanism of Osh4 protein, the study utilizes and reports findings on recombinant protein production optimization in the yeast Saccharomyces cerevisiae (one of the...
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Efeito protetor da lactoferrina humana no trato gastrintestinalThe document reviews the bioactivity (anti-microbial, anti-inflammatory) and structure of human lactoferrin, a primary target protein. Understanding the native bioactivity is essential for evaluating...
2 claims
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Effect of P to A Mutation of the N-Terminal Residue Adjacent to the Rgd Motif on Rhodostomin Importa2012 · PLOS ONE
Although the target protein (Rhodostomin) is not a human milk protein, the document provides highly detailed and transferable methodological data essential to the research goal. It describes the...
3 claims
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Effect of Pichia pastoris host strain on the properties of recombinant Aspergillus niger endoglucanaJournal of Microbiology
The document directly compares the effect of different host strains (Mut+ vs. MutS Pichia pastoris) within a key expression platform (Pichia) on recombinant protein yield, activity, and biochemical...
3 claims
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Effect of carbon source on the glycosylation pathway of Trichoderma reesei RUT-C30The document investigates the performance (secretion, PTM fidelity/glycosylation pathway) of one of the specified expression platforms (filamentous fungi, specifically Trichoderma reesei). It...
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Effect of carbon source on the glycosylation pathway of Trichoderma reesei RUT-C30The document investigates the fundamental glycosylation pathway (N-glycans, O-glycans, nucleotide sugars) in Trichoderma reesei, which is a key organism under the 'filamentous fungi' expression...
3 claims
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Effect of ovotransferrin and lactoferrins on i Chlamydophila psittaci i adhesion and invasion in HD12007 · Vet. Res.
The research goal requires evaluating the bioactivity of human milk proteins, specifically lactoferrin. This document details specific functional assays (anti-adhesion and anti-invasion) for native...
2 claims
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Effect of lactoferrin protein on red blood cells and macrophages mechanism of parasite&ndash;hosThe research goal requires data on the bioactivity of human milk proteins (lactoferrin). This document provides detailed findings on the mechanism of action, immunomodulatory effects (TLR...
3 claims
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Effector-mediated microbiome manipulation by the soil-borne fungal plant pathogen Verticillium dahli2020
The research goal specifically targets the production and bioactivity of lysozyme. Although the thesis focuses on plant pathology and fungal effectors, Chapter 5 is titled 'Functional...
2 claims
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Effects of Dietary Microbial Muramidase on the Growth, Liver Histoarchitecture, Antioxidant Status,2023
The research goal focuses on the expression of human milk proteins, specifically Lysozyme (Muramidase), using filamentous fungi and bioactivity. This document describes a commercial microbial...
2 claims
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Effects of Rho1, a small GTPase on the production of recombinant glycoproteins in Saccharomyces cere2016
The document investigates strategies (overexpression of RHO1/FKS2) to improve protein production, secretion, N-glycan site occupancy, and cellular robustness in engineered 'humanized' Saccharomyces...
3 claims
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Effects of Signal Peptide and Chaperone Co-Expression on Heterologous Protein Production in Escheric2023
The document investigates strain engineering strategies (signal peptides and chaperone co-expression) to optimize titer and achieve proper folding/secretion in the bacterial platform (E. coli), which...
2 claims
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Effects of adding poly-histidine tag on stability, antimicrobial activity and safety of recombinant2023
The document investigates a core component of the research goal: evaluating bacterial expression platforms (E. coli) for recombinant proteins. Specifically, it details the use of periplasmic...
2 claims
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Effects of different media supplements on the production of an active recombinant plant peroxidase i2015
The document investigates optimization strategies (media supplements, bioprocess development) and strain engineering outcomes specifically within the Pichia pastoris platform, which is a target...
2 claims
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Effects of glycerol supply and specific growth rate on methanol-free production of CALB by P. pastor2017
Classification failed: no response from batch API
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Effects of human donor milk on gut barrier function and inflammation in vitro study of the beneficiaFrontiers in Immunology
The research goal requires comparing the bioactivity and PTM fidelity of recombinant human milk proteins (lactoferrin, alpha-lactalbumin, lysozyme). This document identifies the required bioactivity...
3 claims
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Effects of recombinant human lactoferrin on calcium signaling and functional responses of human neutThe document investigates the bioactivity of recombinant human lactoferrin (rhLf), a target human milk protein, which is one of the core metrics (titer, secretion, PTM fidelity, and bioactivity)...
2 claims
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Efficacious Recombinant Influenza Vaccines Produced by High Yield Bacterial Expression A Solution to2008 · PLOS ONE
The document investigates high-yield bacterial expression (E. coli), which is one of the platforms in the research goal. Critically, it provides findings on maximizing titer/efficiency and addresses...
2 claims
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Efficient N-Glycosylation of the Heavy Chain Tailpiece Promotes the Formation of Plant-Produced Dime2020 · Frontiers in Chemistry
The document investigates the production and assembly of dimeric IgA, a precursor to the target protein sIgA, focusing intensely on key metrics required by the goal: PTM fidelity (N-glycosylation...
3 claims
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Efficient Production of Recombinant Protegrin-1 From Pichia pastoris, and Its Antimicrobial and in v2018 · Frontiers in Microbiology
The document investigates the efficient production of a recombinant protein in Pichia pastoris, one of the key expression platforms specified in the research goal. It provides transferable data...
3 claims
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Efficient Secretion of Recombinant Proteins from Rice Suspension-Cultured Cells Modulated by the Cho2015 · PLOS ONE
Although the platform (rice) is outside the microbial scope of the research goal, the document focuses heavily on detailed strain construction elements (optimization of signal peptides) to maximize...
2 claims
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Efficient autotransporter-mediated extracellular secretion of a heterologous recombinant protein byThe document investigates high-titer extracellular secretion of a heterologous protein in one of the required expression platforms (bacteria/E. coli), providing detailed strain engineering data...
3 claims
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Efficient expression of viral surface proteins by transient gene expression in Hi5 insect cells for2017
Although the document focuses on viral surface proteins expressed in insect cells (Hi5), the core methodologies address the key metrics of the research goal: efficient recombinant glycoprotein...
3 claims
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Efficient extracellular expression and rapid screening of human-like recombinant gelatin in KomagataThe document focuses on optimizing titer/secretion in a key target platform (Komagataella phaffii/Pichia pastoris). It details transferable strain engineering methods, specifically using high-copy...
3 claims
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Efficient increase of the novel recombinant human plasminogen activator expression level and stabiliThe document investigates expression efficacy and stability (titer) using mammary-specific regulatory elements (goat beta-casein promoter) to produce a recombinant protein. Although the platform is...
2 claims
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Efficient production of human growth factors in Escherichia coli by fusing with small protein 6HFh8The document details highly efficient production methods (g/L titers, solubility tags, fed-batch fermentation) for recombinant human proteins in the bacterial expression platform (E. coli). This...
3 claims
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Efficient production of human growth factors in Escherichia coli by fusing with small protein 6HFh8The document investigates the efficient production of recombinant human proteins (Growth Factors) in E. coli (a platform listed in the research goal). It provides detailed strain engineering data...
3 claims
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Efficient recombinant production of mouse-derived cryptdin family peptides by a novel facilitation s2023
The document investigates recombinant protein production in E. coli (a target platform) and critically employs Human alpha-lactalbumin (HLA), which is a key target protein listed in the Research...
3 claims
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Efficient secretory expression of type III recombinant human collagen with triple-helical structure2025
Although the specific protein is collagen, not HMP, this document provides highly detailed, transferable engineering data essential for the research goal. It focuses on optimizing secretory...
3 claims
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Efficient single step chromatographic purification of recombinant human antithrombin (rhAT) from Sac2016
The document utilizes one of the target expression platforms (Saccharomyces cerevisiae) to produce a complex recombinant bioactive glycoprotein (Antithrombin). Although the specific protein is not a...
2 claims
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Efficient soluble expression of disulfide bonded proteins in the cytoplasm of Escherichia coli in fe2017
The document investigates the bacterial expression platform (E. coli) for high-yield, large-scale production (fed-batch fermentation, high titer) of complex disulfide-bonded recombinant proteins,...
3 claims
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Einfluß niedermolekularer Medienzusätze und sezernierter, ATP-unabhängiger Chaperone auf die AusbeutProf. Dr.
The document, a PhD thesis on E. coli expression, provides detailed strain engineering outcomes (co-secretion of chaperones, media optimization) focusing on improving the titer, secretion, and proper...
2 claims
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Enabling Low Cost Biopharmaceuticals A Systematic Approach to Delete Proteases from a Well-Known Pro2015 · PLOS ONE
The document provides detailed strain construction and engineering outcomes for a filamentous fungus (Trichoderma reesei), which is one of the target expression platforms. The research focuses on...
3 claims
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Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-The document focuses entirely on strain engineering and process optimization (promoter identification, titer comparison, methanol-free systems, fed-batch optimization) within Komagataella phaffii...
3 claims
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Enabling low cost biopharmaceuticals high level interferon alpha-2b production in Trichoderma reesei2016
The document investigates detailed strain engineering (multiple protease deletions) in a target expression platform (filamentous fungi, Trichoderma reesei) to maximize protein titer and secretion (up...
3 claims
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EndoE from Enterococcus faecalis Hydrolyzes the Glycans of the Biofilm Inhibiting Protein Lactoferri2014 · PLOS ONE
The research goal focuses heavily on the PTM fidelity (glycosylation) and bioactivity of human milk proteins, specifically lactoferrin (hLF). This document directly investigates how the enzymatic...
3 claims
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Engineered Microorganisms for the Production of Food Additives Approved by the European Union—A Syst2018 · Frontiers in Microbiology
The document, while focusing on small molecule food additives, provides a systematic review of engineered microbial production hosts (overlapping with goal platforms like bacteria/yeast) and,...
3 claims
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Engineered β-Lactoglobulin Produced in E. coli Purification, Biophysical and Structural Characterisa2016
The document provides detailed strain construction data and engineering outcomes (DsbC co-expression, N-terminal cleavage mutations) for producing a complex, bioactive milk protein (b-Lactoglobulin)...
3 claims
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Engineering Saccharomyces cerevisiae for medical applications2025
The document is a comprehensive review focusing on the genetic engineering and use of Saccharomyces cerevisiae (one of the target expression platforms) as a cell factory for pharmaceuticals. It...
3 claims
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Engineering Escherichia coli for soluble expression and single step purification of active human lys2012
The research goal specifically requires assessing 'regulatory viability' for human milk proteins like Lysozyme. This document is a European Commission Regulation detailing the regulatory status,...
2 claims
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Engineering of a Potent Recombinant Lectin-Toxin Fusion Protein to Eliminate Human Pluripotent Stem2017
The research goal requires comparison across different expression platforms, including bacteria. This document provides concrete data on the production (yield/titer: 9 mg/L, expressed in the soluble...
2 claims
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Engineering of the unfolded protein response pathway in Pichia pastoris enhancing production of secr2021
The document focuses on engineering the Pichia pastoris expression platform (a key platform in the research goal) to enhance titer, secretion, protein folding, and proper post-translational...
3 claims
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Engineering recombinant antibodies for polymer biofunctionalizationThe document investigates engineering the bacterial expression platform (E. coli) to achieve site-specific glycosylation (PTM fidelity) of recombinant proteins using heterologous machinery (C. jejuni...
3 claims
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Engineering the flagellar type III secretion system improving capacity for secretion of recombinantThe document provides highly detailed strain construction data (gene knockouts, plasmid maps, signal peptide variations based on fliC/flg systems, promoters/vectors like pTrc99a/pJex) specifically...
3 claims
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Engineering the flagellar type III secretion system improving capacity for secretion of recombinant2019
The document investigates detailed strain engineering and signal peptide modification in E. coli (a targeted expression platform) to maximize extracellular secretion titer, providing directly...
2 claims
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Engineering the flagellar type III secretion system improving capacity for secretion of recombinant2019
The research goal specifically includes bacteria as an expression platform and requires data on titer and secretion mechanisms/optimization. This document provides detailed strain engineering data...
2 claims
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Enhanced Production of Bovine Chymosin by Autophagy Deficiency in the Filamentous Fungus Aspergillus2013 · PLOS ONE
The document details successful strain engineering (autophagy deficiency) in a relevant expression platform (filamentous fungus, Aspergillus oryzae) to significantly enhance the titer and secretion...
2 claims
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Enhanced Production of Recombinant Secretory Proteins in Pichia pastoris by Optimizing Kex2 P1’ site2013 · PLOS ONE
The document focuses on enhancing the secretion titer of recombinant proteins in Pichia pastoris, which is one of the key expression platforms explicitly listed in the research goal. The findings...
2 claims
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Enhanced antifungal activity of bovine lactoferrin-producing probiotic Lactobacillus casei in the muThe document investigates the heterologous expression and secretion of lactoferrin (a target milk protein) in a bacterial expression platform (Lactobacillus casei), using a specific secretion vector...
3 claims
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Enhanced cell density cultivation and rapid expression-screening of recombinant Pichia pastoris clon2020
The document details high-throughput screening (HTS) and optimization methods for high cell density cultivation in *Pichia pastoris*, a target expression platform. This directly informs the research...
3 claims
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Enhanced cell disruption strategy in the release of recombinant hepatitis B surface antigen from PicThe document provides highly transferable methods for optimizing the efficiency of protein recovery (specific protein release/titer) in the Pichia pastoris expression platform, which is one of the...
3 claims
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Enhanced delignification of lignocellulosic substrates by <i>Pichia< i> GS115 expressed2018 · Gen. Appl. Microbiol.
The document provides detailed technical data on one of the target expression platforms, Pichia pastoris (GS115), using the pPIC9K vector and AOX promoter. It includes specifics on codon...
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Enhanced extracellular recombinant keratinase activity in i Bacillus subtilis i SCK6 through signalThe document investigates enhanced protein secretion and titer in a relevant bacterial expression platform (Bacillus subtilis) through detailed strain engineering methods, specifically signal peptide...
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Enhanced extracellular production of alpha-lactalbumin from i Bacillus subtilis i through signal pep2024
The document reports detailed strain engineering (signal peptide and promoter screening) for enhanced expression and secretion of human alpha-lactalbumin (a target protein) in Bacillus subtilis (a...
3 claims
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Enhanced secretion of a methyl parathion hydrolase in Pichia pastoris using a combinational strategy2015
The document investigates detailed strain engineering strategies (N-terminal fusion tags, modification of ER retention signals like KKXX, stability mutations) to optimize secretion titer of a...
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Enhancement of Fungal Enzyme Production by Radio-Frequency Electromagnetic Fields2022
The document investigates a physical method (RF-EMF) to enhance the titer and extracellular secretion of an enzyme (alpha-amylase) in a filamentous fungus (Aspergillus oryzae). Filamentous fungi are...
3 claims
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Enhancement of the Trichoderma reesei expression systemThe document provides detailed strain engineering data for the filamentous fungi platform (Trichoderma reesei), which is one of the required expression systems. It specifically addresses critical...
2 claims
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Enhancement of the Trichoderma reesei expression systemThe document focuses entirely on enhancing the heterologous expression system in Trichoderma reesei (a filamentous fungus, one of the target platforms). It specifically addresses key metrics relevant...
2 claims
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Enhancing biomolecule production through microbial engineering and advanced manufacturingThe dissertation focuses on 'Enhancing Biomolecule Production through Microbial Engineering' using key expression platforms specified in the research goal (E. coli/Bacteria and Saccharomyces...
3 claims
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Enhancing recombinant thaumatin production by Komagataella phaffii through cold atmospheric helium pThe document investigates methods to significantly enhance recombinant protein titer and secretion in Komagataella phaffii (Pichia pastoris), which is one of the target expression platforms. The...
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Enhancing the expression of recombinant proteins in the methylotrophic yeast Pichia pastorisProf. Dipl.
The document is a detailed PhD dissertation focused on optimizing the Pichia pastoris expression platform (a key platform in the research goal). It provides methods for enhancing titer and addressing...
2 claims
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Enhancing the selective extracellular location of a recombinant E. coli domain antibody by managemen2015
The document investigates methods (fermentation management, chemical agents like PEI) to enhance selective extracellular secretion and improve primary recovery (titer, reduced nucleic acid release)...
2 claims
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Enterotoxigenic Escherichia coli infection of weaned pigs Intestinal challenges and nutritional inte2022 · Frontiers in Immunology
The document reviews the utilization and efficacy (bioactivity) of lysozyme, which is one of the bioactive human milk proteins explicitly listed in the research goal. While the document focuses on...
2 claims
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Entwicklung neuartiger Influenza-A-subunit-Impfstoffe, basierend auf rekombinanter Kluyveromyces lacProf. Dr.
The document is a PhD thesis focusing on heterologous protein expression using a relevant yeast system (*Kluyveromyces lactis*). Although the target protein is an Influenza antigen, the research goal...
3 claims
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Enzymatic Production of Glucosylxylose Using a Cellobiose Phosphorylase-Yeast Combined System2010 · Appl. Glycosci.
The document provides detailed strain construction data, promoter information (AmyB promoter), and specific engineering outcomes (successful intracellular expression, failed extracellular expression)...
2 claims
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Enzymatic fine-tuning for 2-(6-hydroxynaphthyl) β-d-xylopyranoside synthesis catalyzed by the recomb2016
The document utilizes Pichia pastoris, a target expression platform, to produce a recombinant protein. It directly addresses key research metrics by reporting secretion yield (8 U/mL) and providing...
3 claims
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Enzyme-Based Anti-Inflammatory Therapeutics for Inflammatory Diseases2025
The document, while focused on anti-inflammatory therapeutics, explicitly discusses the recombinant production of enzymes (including lysozyme, a target human milk protein) in several required...
3 claims
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Enzyme-catalyzed protein crosslinking2012
The document reviews enzyme-catalyzed protein crosslinking, ligation, and post-translational modification (PTM) strategies (e.g., Transglutaminase, Sortase A). These enzymatic methods are highly...
3 claims
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Enzymes in medical applications investigating the enzyme horseradish peroxidaseProf. Dipl.
The document investigates key aspects of the research goal, specifically comparing expression platform performance (P. pastoris) regarding PTM fidelity and strain engineering. Chapters II and III...
3 claims
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Erratum Ntana, F., et al. Aspergillus A Powerful Protein Production Platform. Catalysts 2020, 10, 102020
The document, although an erratum, directly references strain engineering methods (promoters, protease deletion, degradation pathway modification) and production outcomes (titer/mg/L) within a...
3 claims
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Escherichia coli Reporter Strains Allow for the In Vivo Evaluation of Recombinant Elongation Factor2024 · Appl. Microbiol.
The document investigates critical factors for optimizing bacterial expression platforms (E. coli), specifically focusing on overcoming translational bottlenecks (polyproline stalling) and the role...
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Established tools and emerging trends for the production of recombinant proteins and metabolites inThe research goal specifically investigates the use of Pichia (Pichia pastoris/Komagataella phaffii) as an expression platform for recombinant proteins. This document is a state-of-the-art review...
3 claims
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Establishing site-specific recombination mediated cassette exchange in Pichia pastoris and developme2015
The document provides detailed strain construction data, engineering methods (Recombinase Mediated Cassette Exchange, RMCE), and optimization protocols for Pichia pastoris, which is one of the key...
3 claims
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Establishment and application of an RNAi system in Pichia pastorisThe document focuses on engineering the Pichia pastoris expression platform (one of the key platforms in the research goal) to improve heterologous protein secretion. Specifically, it uses RNAi to...
2 claims
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Establishing the red microalga Porphyridium purpureum as a novel platform for the production of reco2023 · Prof. Dr.
The document is a detailed PhD dissertation establishing a novel microbial expression platform (red microalga Porphyridium purpureum). While the platform itself (microalgae) and the specific target...
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Establishment of Corynebacterium glutamicum for the heterologous production of bacteriocinsProf. Dr.
The document, a PhD thesis on heterologous protein production in a bacterial host (*Corynebacterium glutamicum*), provides detailed engineering data relevant to the research goal. Specifically, it...
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Establishment of Human Lysozyme Mass Production System Using Insect Factory, Silkworm Larvae2009
The document focuses on producing Human Lysozyme (a target protein) and explicitly uses the expression systems Pichia pastoris and Saccharomyces cerevisiae (target platforms) as benchmarks, providing...
2 claims
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Establishment of a yeast-based VLP platform for antigen presentation2018
The document investigates high-yield heterologous protein expression (VLPs) in a key platform specified in the research goal (*Hansenula polymorpha*/Pichia). The findings regarding strain...
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Estudo da eficiência da lactoferrina como conservante em formulações semi-sólidas para produtos cosmProfa. Dra.
The document investigates the bioactivity (antimicrobial efficacy) of lactoferrin, which is one of the target human milk proteins. Although the document focuses on pharmaceutical formulation science...
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Estudo sobre a expressão dos genes de alfa amilase de <i>Bacillus stearothermophilus< i>Prof. Dr.
The document provides a detailed comparative study of heterologous protein expression and secretion efficiency (including use of signal peptides, vector details, and achieving activity) in Bacteria...
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Etablierung eines mikrobiellen Kultivierungsprozesses für die Duft- und Aromastoffproduktion zur digProf. Dr.
The document details bioprocess development, yield optimization (titer), and the use of digital methods/machine learning algorithms to maximize recombinant production in Escherichia coli. Although...
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Etablierung von neuen Methoden zur Herstellung rekombinanter Antikörper und zur spezifischen SelektiProf. Dr.
This PhD thesis details the establishment and optimization of heterologous protein expression (antibody fragments, Fab, scFv) in key expression platforms listed in the research goal, specifically...
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Eukaryotic protein glycosylation a primer for histochemists and cell biologists2016
The research goal specifically requires comparison of PTM fidelity (glycosylation) across different expression platforms (yeast, fungi, bacteria). This document provides a fundamental, detailed...
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Evaluation of the Anti-Inflammatory and Anti-Oxidative Effects of Therapeutic Human Lactoferrin FragThe document investigates the bioactivity (anti-inflammatory and anti-oxidative effects) of recombinant human lactoferrin fragments. It explicitly details the production and purification methods...
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Expression of Recombinant Antibodies2013
The document extensively reviews the performance (titer, secretion, PTM fidelity/glycosylation) of all major expression platforms cited in the research goal (bacteria, yeast, filamentous fungi) for...
3 claims
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Evaluation of the potential food allergy risks of human lactoferrin expressed in Komagataella phaffi2024 · Frontiers in Immunology
The document evaluates recombinant human lactoferrin (a target protein) expressed in Komagataella phaffii (Pichia, a target platform). It specifically discusses the results of glycoengineering (PTM...
3 claims
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Evaluation of the protozoan parasite L. tarentolae as a eukaryotic expression host in biomedical resProf. Dr.
The research goal is centered on comparing the performance of different microbial expression platforms (titer, secretion, PTM fidelity). This document is a detailed evaluation (PhD thesis) of the...
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Evaluation of the α-casein (CSN1S1) locus as a potential target for a site-specific transgene integr2022
The document provides detailed regulatory and expression data (titer, secretion, promoter effectiveness) related to a key target protein locus, alpha-S1-casein (CSN1S1), listed in the research goal...
2 claims
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Evaluation of three different vectors on human iLRP expression in Escherichia coliThe document investigates optimization strategies (codon optimization, vector design, and tags) to maximize the expression titer of a heterologous human protein (iLRP) in the bacterial host E. coli....
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Evaluation of three different vectors on human iLRP expression in Escherichia coliAlthough the target protein (iLRP) is not a human milk protein, the document focuses on detailed strain engineering outcomes and methodologies (codon optimization, vector comparison, tag effects, and...
3 claims
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Evidence of a Bacterial Receptor for Lysozyme Binding of Lysozyme to the Anti-σ Factor RsiV Controls2014
The document investigates the interaction of a target human milk protein (lysozyme) with the secretory and sensing machinery (anti-sigma factor, signal peptidase, RIP system) in a relevant expression...
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Evolution of the vertebrate goose-type lysozyme gene familyThe document focuses on the evolution of vertebrate lysozyme g, a member of the broader lysozyme family (a target protein). While evolutionary, it provides detailed structural and functional analysis...
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Experimental study of the influence of recombinant human lactoferrin on the levels of androgens andThe document investigates the bioactivity (effect on steroidogenesis) of recombinant human lactoferrin (a target protein). Crucially, the introduction details the expression platform (transgenic...
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Exploitation of the twin-arginine translocation pathway and promoter control for biopharmaceutical pThis PhD thesis details strain engineering methods (promoters, signal peptides, Tat/Sec pathway exploitation) in E. coli (a target platform) to improve secretion, folding (disulfide bonds), and titer...
2 claims
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Exploiting bacterial glycosylation machineries for the synthesis of a Lewis antigen-containing glycoThe document directly addresses two key components of the research goal: comparing different expression platforms (bacterial vs. yeast) and analyzing PTM fidelity (glycosylation). It describes...
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Exploration of Factors Affecting Recombinant Protein Secretion in Saccharomyces Cerevisiae2022
The document is a detailed PhD thesis (2022) focused entirely on optimizing recombinant protein secretion in Saccharomyces cerevisiae, which is a required expression platform. It covers detailed...
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Exploring the Potential of Lactoferrin in Neonatology and Obstetrics - Promising Advancements for MaJournal of Education
The document is a review focusing on the bioactivity and clinical applications of Lactoferrin in neonatology and infant health. Although it does not discuss recombinant expression platforms or strain...
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Exploring the Tat pathway for the production of disulphide-bonded recombinant proteins using CyDisCo2023
This PhD thesis explores optimizing the production and secretion (using the Tat pathway and CyDisCo) of disulfide-bonded recombinant proteins in Escherichia coli. This directly addresses key...
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Exploring the modulatory role of bovine lactoferrin on the microbiome and the immune response in hea2024 · Journal of Animal Science and Biotechnology
The document focuses on the bioactivity (microbiome and immune modulation) of bovine lactoferrin in piglets, which is highly relevant to the 'bioactivity for infant nutrition' requirement specified...
2 claims
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Exploring the secretome of Corynebacterium glutamicum ATCC 13032.The document investigates the use of a bacterial expression platform (Corynebacterium glutamicum) for recombinant protein production, specifically focusing on optimizing secretion and titer. It...
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Exploring the therapeutic potential of recombinant human lysozyme a review on wound management systeThe document, particularly Section 2, directly addresses the production of one of the target human milk proteins (recombinant human lysozyme, rhLZM). It explicitly compares expression platforms (E....
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Expressing anti-HIV VRC01 antibody using the murine IgG1 secretion signal in Pichia pastoris2017
The document investigates key parameters for recombinant protein production—titer, secretion efficiency, and secretory stress—in Pichia pastoris, a primary expression platform listed in the goal....
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Expressing recombinant human lactoferrin with antibacterial activity in <i>Nicotiana benthamia2023
The document focuses on producing human lactoferrin (a target protein) and confirms its bioactivity (antibacterial activity). Critically, the introduction provides specific comparative data (titer)...
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Expression, Purification, and Characteristic of Tibetan Sheep Breast Lysozyme Using Pichia pastorisAnim. Sci.
Vol.
The document describes the expression of a key target protein (Lysozyme) using one of the required expression platforms (Pichia pastoris). It provides specific data on titer/secretion rate (>500...
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Expression, characterization, and application potentiality evaluation of recombinant human-like coll2022
Although the target protein is collagen (not a human milk protein), the document provides highly relevant technical data for one of the required expression platforms, Pichia pastoris. It details...
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Expression, purification, and bioactivity of a soluble recombinant ovine interferon-tau in i EscheriThe document provides detailed methodological and outcome data (vector construction, IPTG induction, achieving soluble expression, purification yield, and bioactivity) for one of the specific...
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Expression, purification, and study on the efficiency of a new potent recombinant scFv antibody agai2023
Although the target protein is an scFv antibody against SARS-CoV-2, the document provides detailed methodological data on optimizing recombinant protein expression, secretion, and purification...
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Expression, purification and characterization of a recombinant Tat47–57-Oct4 fusion protein in PichiThe document provides highly detailed, quantifiable strain engineering data (promoter AOX1, specific HSA signal peptide, titer ~210 mg/L, pilot-scale fermentation) for the secretion of a recombinant...
3 claims
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Expression, purification and characterization of the recombinant cysteine-rich antimicrobial peptideJapan.
Tel.
The document investigates the expression performance of a required expression platform (Pichia pastoris) concerning key metrics of the research goal (titer, secretion, and Post-Translational...
2 claims
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Expression, purification and evaluation of recombinant L-asparaginase in mehthylotrophic yeast PichiViet. Env.
The document investigates L-asparaginase expression, secretion, purification, and post-translational modification (glycosylation) in the key expression platform Pichia pastoris. Although the target...
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Expression Strategy of Soluble Recombinant Human TGF-β3 in Escherichia coli sfGFP -Fusion Tag2023 · Journal of Science
The document investigates expression strategies (fusion tags, high yield, solubility enhancement) and engineering outcomes for producing a recombinant human protein in E. coli, which is one of the...
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Expression and Characterization of Rice-produced Recombinant Porcine Lactoferrin and its AntioxidantThe document focuses on a target protein (Lactoferrin) and provides detailed analysis of key metrics required by the research goal: Post-Translational Modification (PTM) fidelity (N-glycosylation...
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Expression and Identification of a Novel High-Activity Recombinant Humanized Type I Collagen SynthCoThe document investigates Pichia pastoris (a target platform) for the high-yield, large-scale production (15.3 g/L in 500L) of a complex recombinant protein requiring specific post-translational...
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Expression and Purification of Hybrid LL-37Tα1 Peptide in Pichia pastoris and Evaluation of Its Immu2019 · Frontiers in Immunology
The document investigates peptide expression in Pichia pastoris, one of the target platforms. Although the specific protein (LL-37Tα1) is not a target human milk protein, the paper provides directly...
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Expression and Purification of Natural N-Terminal Recombinant Bovine Pancreatic Trypsin Inhibitor frThe document, while focusing on BPTI (not a human milk protein), provides highly specific and transferable data crucial for the research goal. It directly compares expression platforms (Pichia, S....
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Expression and analysis of the glycosylation properties of recombinant human erythropoietin expresseThe document provides highly specific and transferable data relevant to comparing expression platforms. It utilizes Pichia pastoris and discusses its advantages over Saccharomyces cerevisiae...
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Expression and characterization of Trichoderma reesei endoglucanase II in Pichia pastoris under the2020 · Journal of Biotechnology
The document provides specific data on strain construction (GAP promoter, vector details) and expression optimization (fed-batch, titer, stability) in one of the key required hosts (*Pichia...
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Expression and characterization of a novel 1,3-regioselective cold-adapted lipase from Rhizomucor en2016
Although the product is a lipase, the document provides detailed, transferable data on the expression performance of a key target platform (*Pichia pastoris*). It includes high titer results (1.28...
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Expression and characterization of recombinant IL-1Ra in Aspergillus oryzae as a system2023 · Ra. Asp.
The document compares two expression platforms relevant to the research goal (filamentous fungus A. oryzae vs. bacteria E. coli). It directly addresses key metrics: titer (53 mg/L), secretion...
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Expression and characterization of recombinant single chain beta-alpha equine chorionic gonadotropinAnim. Res.
The document details the production, purification, and characterization of a complex glycoprotein (eCG) in a specific prokaryotic host (E. coli), which is one of the expression platforms listed in...
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Expression and characterization of two recombinant mammalian metalloproteins b bovine microsomal cytThe document investigates the expression and characterization of Human Serum Transferrin (a metalloprotein highly related to the target protein Lactoferrin) in prokaryotic (E. coli) and eukaryotic...
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Expression and purification of different elastin like polypeptides (ELPs) constructs for therapeuticThe document details expression, purification, scale-up, and critical quality control (endotoxin removal) of recombinant proteins using the E. coli platform. Although the protein (ELP-Lacritin) is...
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Expression and purification of extracellular domain of human CD33 in Escherichia coli and Pichia pasThe document is a detailed thesis comparing two primary expression platforms specified in the research goal (Pichia pastoris and E. coli/Bacteria). It provides detailed strain construction data...
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The technology of pharmingThe document discusses critical comparison metrics for the research goal, including the importance of post-translational modifications (PTMs, specifically glycosylation) for protein function, the...
3 claims
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Expression and purification of recombinant human granulocyte colony‐stimulating factor (rG‐CSF) from2024 · Journal of Biotechnology
The document addresses one of the required expression platforms (Pichia pastoris) and provides highly transferable data on key metrics for the research goal, including optimization for high...
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Expression einer funktionellen rekombinanten Glykosyltransferase in Escherichia coli2015 · Prof. Dr.
The research goal requires studying PTM fidelity (glycosylation) across different expression platforms, including bacteria (E. coli). This PhD dissertation provides detailed technical data, methods,...
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Expression of Active Fluorophore Proteins in the Milk of Transgenic Pigs Bypassing the Secretory PatThe study focuses on a non-target expression platform (transgenic pigs), but the introduction provides high-yield benchmark data for the target proteins (lactoferrin, alpha-lactalbumin, lysozyme) in...
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Expression of Bioactive Lunasin Peptide in Transgenic Rice Grains for the Application in Functional2018
The document's core subject (Lunasin in transgenic rice) is outside the scope of the research goal (Human Milk Proteins in microbial systems). However, the introduction explicitly mentions and cites...
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Expression of FMD virus-like particles in yeast Hansenula polymorpha and immunogenicity of combine w70
The document details the construction and expression of a recombinant protein (VLP) in a key target platform (*Hansenula polymorpha*, a methylotrophic yeast). It provides specific data on strain...
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Expression of Glycosylated Mucin–like Domain Using Baculovirus Expression System in Silkworm, BombyxThe document provides detailed methodological findings on achieving complex post-translational modifications (PTMs), specifically Core 1 and Core 2 O-glycosylation, in a heterologous eukaryotic...
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Expression of Pisum sativum defensin 1 (Psd1) in shaking flasks and bioreactor cultivations of recomThe document investigates optimization strategies (fed-batch, pH, bioreactor scale-up, modeling) for maximizing the secretion and titer of a recombinant protein using Pichia pastoris, which is one of...
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Expression of Recombinant Hirudin in Bacteria and Yeast A Comparative Approach2025
The document provides a direct comparative analysis of two key expression platforms specified in the research goal (Yeast/Pichia pastoris and Bacteria/E. coli). It specifically addresses critical...
3 claims
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Expression of Recombinant Human Lysozyme in Egg Whites of Transgenic Hens2015 · PLOS ONE
Studies the successful expression and bioactivity (titer, activity comparison) of one of the specific target human milk proteins (lysozyme). While the expression system is transgenic hens (not...
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Expression of Recombinant Hypoallergenic Sui p 2 in Saccharomyces boulardii, a Potential Prophylacti2025
The document provides detailed, transferable strain construction data (TEF1 promoter, alpha-factor signal peptide, integration strategy via pYIP) and initial expression outcomes in one of the target...
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Expression of Recombinant Rat Secretable FNDC5 in Pichia Pastoris and Detection of Its Biological AcFrontiers in Endocrinology
The document investigates one of the key expression platforms (*Pichia pastoris*) mentioned in the research goal. It provides detailed methodology on achieving high yield, secretion, and biological...
2 claims
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Expression of a Functional Recombinant Human Basic Fibroblast Growth Factor from Transgenic Rice See2013 · Mol. Sci.
The document primarily focuses on bFGF in transgenic rice, which is not a target protein or a microbial platform. However, the introduction discusses difficulties encountered when expressing proteins...
2 claims
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Expression of a ScFv–E2T fusion protein in CHO-K1 cells and alfalfa transgenic plants for the selectThe document, while focusing on a viral protein in CHO cells and alfalfa, details methodologies highly relevant to the research goal, specifically regarding maximizing titer and secretion. This...
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Expression of a Recombinant Lentinula edodes Xylanase by Pichia pastoris and Its Effects on Ruminal2018 · Frontiers in Microbiology
The document details the cloning and expression of a recombinant protein using one of the required expression platforms (Pichia pastoris), and provides relevant technical information (strain X-33,...
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Expression of a new serpin gene screening from metagenomics database in ( textit{Pichia pastoris} )2021 · Journal of Biology
The research goal is to compare different expression platforms (Pichia, Saccharomyces) regarding titer, secretion, and PTM fidelity (glycosylation). Although the target protein is not a Human Milk...
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Expression of bovine lactoferrin N-lobe by the green alga, Chlorella vulgarisThe document investigates the expression of a key target protein (lactoferrin) and provides detailed strain construction data (codon optimization, promoters, vectors) explicitly requested in the...
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Expression of endo-1, 4-beta-xylanase from Trichoderma reesei in Pichia pastorisand functional chara2009
Although the target protein is not a human milk protein, the document provides detailed, high-quality engineering and performance data (titer, secretion purity, promoter system, and multicopy...
3 claims
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Expression of human lactoferrin N-lobe in Pichia pastoris and its antibacterial activity2015 · Journal of Microbiology
The document describes the expression of a bioactive human milk protein (lactoferrin N-lobe) in one of the specified expression platforms (Pichia pastoris). It provides quantitative data on titer...
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Expression of i Bacillus ginsengihumi i M2.11 bacterial phytase by recombinant Pichia i pastoris str2021
The document focuses on optimizing the expression and secretion of a recombinant protein in Pichia pastoris (a required platform) using detailed strain engineering data. It specifically compares the...
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Expression of kiwifruit-derived actinidin in Nicotiana benthamiana leavesFrontiers in Plant Science
While the primary expression platform (Nicotiana benthamiana) is not one of the microbial systems listed in the goal, the study provides transferable data regarding protein engineering (use of BiP...
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Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86The document investigates the performance, titer, and secretion strategy for expressing a complex recombinant protein in Pichia pastoris, which is one of the key expression platforms specified in the...
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Expression of recombinant human lactoferrin in transgenic alfalfa plantsAlthough the platform is transgenic alfalfa (plant) and not one of the specified microbial systems (Pichia, bacteria, etc.), the document provides highly relevant comparative data on a target human...
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Expression of recombinant human lysozyme-tachyplesin I (hLYZ-TP I) in Pichia pastoris and analysis oThe document directly studies the expression of a core target human milk protein (lysozyme) in one of the specified expression platforms (Pichia pastoris). It details the use of a signal sequence...
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Expression of recombinant human lysozyme in transgenic chicken promotes the growth of Bifidobacteriu2016
The document focuses on a target human milk protein (lysozyme) and provides essential metrics: expression titer (29.9 μg/mL in egg white) and detailed bioactivity data (antibacterial assay, promotion...
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Expression of recombinant porcine lactoferrin N-lobe in <i>Pichia< i> <i>methanoli2007 · Journal of Animal and Feed Sciences
The document investigates the expression and secretion of lactoferrin (a target protein class) in Pichia (a target expression platform). It provides detailed strain engineering data (promoters,...
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Expression of recombinant staphylokinase in the methylotrophic yeast Hansenula polymorphaThe document investigates a relevant expression platform (Hansenula polymorpha, a methylotrophic yeast) and provides highly transferable data regarding key metrics of the research goal: optimizing...
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Expression of the recombinant Blo t 1 allergen from Blomia tropicalis in Pichia pastoris and its IgE2025 · Journal of Agriculture and Biology
The document focuses on expressing a dust mite allergen (Blo t 1) instead of human milk proteins. However, it uses one of the target platforms (Pichia pastoris) and provides highly specific...
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Expression of the recombinant single chain variable fragments recognizing blood antigen fused with t2019
The document investigates crucial strain engineering outcomes (using Thioredoxin fusion tag) to optimize the expression and solubility of a recombinant protein in the bacterial platform (E. coli). E....
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Expression of the secretory form of recombinant regucalcin ofBubalus bubalis in methylotropic yeastAnim. Res.
The document investigates the expression and characterization (including detailed cloning vectors/methods) of a secretory protein in Pichia pastoris, which is one of the target expression platforms....
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Expression of Δ-desaturase Gene in a Recombinant Pichia pastoris GS115 Strain and Its Activity2011
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Expression tuning in E.coli discussion of state-of-the-art technologies and multivariate investigatiIng. Dr.
The document investigates expression tuning systems (pBAD, pLac, pRha) and fermentation strategies (mixed feed, DOE) in E. coli to optimize recombinant protein production, focusing on maximizing...
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Expression tuning in Escherichia coli via a glucose lactose mixed feed systemIng. Dr.
The document focuses on optimizing key metrics (titer, soluble protein yield, reduced metabolic burden) for recombinant protein production in Escherichia coli using controlled feeding strategies and...
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Expression von Tropoelastin und Lysinoxidase in PflanzenThe document investigates the expression of recombinant human proteins (Tropoelastin and Lysine Oxidase) using two platforms specified in the research goal: E. coli (bacteria) and Pichia pastoris...
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Expressão, purificação e avaliação imunológica de formas truncadas e hibrídos da proteína de superfíProfa. Dra.
Although the main subject of the thesis is the expression and immunological evaluation of PspA (a vaccine candidate), the document explicitly discusses Lactoferrin (ALF/HLF), which is one of the key...
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Extracellular Expression of Recombinant Kex2 Protease in Pichia pastoris BG10The document provides a detailed case study of recombinant protein (Kex2 protease) expression in a key platform specified in the goal (*Pichia pastoris*). It contains specific and transferable...
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Extracellular Production of Glutathione by Recombinant Escherichia coli K-122023 · Microbiol. Res.
The document investigates detailed strain engineering methodologies (promoters like T5, gene cloning, knockout of transporters/degrading enzymes) in a bacterial expression platform (E. coli K-12) to...
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Extracellular Recombinant Protein Production with Escherichia coliAssoc. Prof. Dipl.
The document focuses on optimizing Escherichia coli (a key bacterial expression platform listed in the research goal) for extracellular recombinant protein production, specifically addressing...
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Extracellular expression, purification and bioreactor production of tambaqui (Colossoma macropomum)Although the target protein (fish growth hormone) is incorrect, the document provides detailed, high-quality data on the performance of a crucial expression platform (*Komagataella phaffii*/Pichia...
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Extracellular overexpression of recombinant Thermobifida fusca cutinase by alpha-hemolysin secretionThe document provides detailed strain construction data (alpha-hemolysin secretion system, signal peptides, co-expression of HlyB/D) and engineering outcomes (titer and secretion efficiency) for...
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FACS-Based Functional Protein Screening via Microfluidic Co-encapsulation of Yeast Secretor and Mamm2020
The document presents a novel, ultra-high-throughput FACS-based screening method for identifying bioactive secreted recombinant proteins from yeast secretor cells (Saccharomyces cerevisiae, a target...
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Fabrication of Biofunctionalized Protease-Based Chitosan Collagen Composite Membranes and EfficientProf. Dr.
The document investigates recombinant protein expression, yield (titer), and Post-Translational Modifications (N-glycosylation prediction and molecular weight discrepancy suggesting PTM) in a...
3 claims
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Factors Influencing Recombinant Protein Secretion Efficiency in Gram-Positive Bacteria Signal Peptid2019 · Frontiers in Bioengineering
The document directly addresses a critical factor in the research goal: optimizing protein secretion and titer. It focuses on 'signal peptides' and 'secretion efficiency' in 'Gram-Positive Bacteria,'...
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Fate of the UPR marker protein Kar2 Bip and autophagic processes in fed-batch cultures of secretory2018
The document investigates fundamental secretion mechanisms (UPR, Kar2/Bip, autophagy) and their modulation by culture conditions in Pichia pastoris, which is one of the key expression platforms...
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Fc-fusion proteins new developments and future perspectivesThe research goal includes secretory IgA (sIgA), an immunoglobulin. This document is a detailed review of Fc-fusion proteins, their modifications (including IgA, IgE, and IgM fusions, and changes...
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Featured Prebiotic Agent The Roles and Mechanisms of Direct and Indirect Prebiotic Activities of Lac2023
The research goal focuses on the production and bioactivity of human milk proteins like lactoferrin (LF) across different expression platforms. This document is a review specifically about LF and its...
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Features of the biotechnologically relevant polyamide family “cyanophycins” and their biosynthesis iAlthough the document focuses on cyanophycin (CGP) synthesis rather than human milk proteins (HMPs), it provides critical comparative data directly relevant to the research methodology. Table S4...
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Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese wheyThe document investigates optimized fed-batch cultivation strategies (maximizing titer/productivity) and strain construction using Saccharomyces cerevisiae, one of the key expression platforms in the...
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Fed-Batch Production of Saccharomyces cerevisiae L-Asparaginase II by Recombinant Pichia pastoris MU2019 · Frontiers in Bioengineering
The document provides detailed process engineering, titer, and secretion data for heterologous protein production using Pichia pastoris (a key platform listed in the RG). It focuses on fed-batch...
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Fermentation process for recombinant protein production in Komagataella phaffiiProf. Dr.
The document is a PhD thesis focusing on optimizing the fermentation bioprocess for recombinant protein production in Komagataella phaffii (Pichia pastoris), which is a key expression platform listed...
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Fhl1p protein, a positive transcription factor in Pichia pastoris, enhances the expression of recomb2019
The document investigates strain engineering techniques (using the transcription factor Fhl1p) to enhance the titer and secretion of recombinant proteins in Pichia pastoris. Pichia pastoris is one of...
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Flo5-1 and Nrg1 are involved in reversible pH-dependent flocculation in Komagataella phaffii2025
The document focuses on detailed strain engineering (gene deletion, overexpression, promoter usage, integration sites) in Komagataella phaffii (Pichia pastoris), which is a key expression platform...
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Fluorescence-Polarization-Based Assaying of Lysozyme with Chitooligosaccharide Tracers2024
The document provides a new, highly reproducible method (Fluorescence Polarization-Based Assaying) for measuring both the concentration (titer) and enzymatic activity (bioactivity) of human lysozyme....
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Food-Derived Opioid Peptides in Human Health A Review2020
The document reviews food-derived opioid peptides and explicitly mentions 'casexins' (derived from Casein) and 'lactoferroxins' (derived from Lactoferrin). Caseins and Lactoferrin are target human...
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Food-Derived Opioids Production and the Effects of Opioids on Human HealthThe document extensively reviews bioactive peptides (exorphins like casomorphins, lactoferroxins, and lactorphins) derived from the exact target human milk proteins (lactoferrin, alpha-lactalbumin,...
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Four-Week GLP Immunotoxicity Assessment of Lactoferrin Alpha Produced by i Komagataella phaffii i inThe document investigates the safety, toxicokinetics, and immunogenicity (key aspects of 'regulatory viability' and 'bioactivity for infant nutrition') of recombinant human lactoferrin (a core target...
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From strain engineering to process development monoclonal antibody production with an unnatural amin2022
The document investigates strain engineering and process optimization (titer, secretion, promoters, chaperones, knockouts) in Pichia pastoris, which is one of the core expression platforms listed in...
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Full-length versus truncated α-factor secretory signal sequences for expression of recombinant human2023 · Journal of Genetic Engineering and Biotechnology
The document provides critical, transferable technical data regarding strain construction and optimization in one of the required expression platforms (Pichia pastoris). Specifically, it compares the...
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Functional analysis of recombinant human and Yarrowia lipolytica O-GlcNAc transferases expressed inThe document focuses on the comparative genomic analysis and specific genetic engineering (deletion strategy) of O-linked N-acetylglucosamine transferase (OGT) in various fungi, including the yeast...
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Functional characterisation of a recombinant xylanase from i Pichia pastoris i and effect of the enz2010
Although the expressed protein is xylanase, the document provides detailed, practical methods and results for high-titer production (fermentation protocol, titer metrics, scale-up to 15L) in Pichia...
3 claims
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Functional expression of aryl‐alcohol oxidase in i Saccharomyces cerevisiae i and i Pichia pastorisThe document compares two key expression platforms specified in the research goal (Saccharomyces cerevisiae and Pichia pastoris) using relevant comparative metrics. It provides detailed data on...
3 claims
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Functional insights from recombinant production of bacterial proteases in Saccharomyces cerevisiae2025
The document investigates Saccharomyces cerevisiae, one of the key expression platforms specified in the research goal, focusing on optimizing strain engineering outcomes. It provides valuable...
3 claims
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Functional production of human antibody by the filamentous fungus Aspergillus oryzaeThe document investigates the production of a complex human glycoprotein (antibody) in one of the target expression platforms (filamentous fungi, Aspergillus oryzae). It provides detailed and...
3 claims
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Functional production of human antibody by the filamentous fungus Aspergillus oryzae2020
The document investigates a key expression platform (filamentous fungi, Aspergillus oryzae) listed in the research goal. Although the specific protein is an antibody (IgG/Adalimumab) rather than a...
3 claims
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Functional production of human antibody by the filamentous fungus Aspergillus oryzaeThe document investigates the use of a key expression platform listed in the research goal (filamentous fungus, Aspergillus oryzae) and directly addresses the critical metrics: titer (39.7 mg/L),...
3 claims
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Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures whThe document focuses on optimizing recombinant protein production (titer and secretion) in the Pichia pastoris expression system, which is one of the primary platforms specified in the research goal....
2 claims
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FungalBraid 2.0 expanding the synthetic biology toolbox for the biotechnological exploitation of filThe research goal specifically investigates filamentous fungi as an expression platform. This document expands the synthetic biology toolbox (FungalBraid 2.0) for filamentous fungi, validating new...
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Fungal Cell Factories for Efficient and Sustainable Production of Proteins and Peptides2022
This review directly addresses one of the key expression platforms mentioned in the research goal: filamentous fungi. It compares fungal platforms to yeast and bacteria regarding crucial metrics like...
3 claims
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Further enhanced production of heterologous proteins by double-gene disruption (ΔAosedD ΔAovps10) inThe document focuses on enhancing heterologous protein production (titer/secretion/stability) through strain engineering (gene disruption of ΔAosedD ΔAovps10) in the filamentous fungus Aspergillus...
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GFP facilitates native purification of recombinant perlucin derivatives and delays the precipitation2012 · PLOS ONE
The document investigates optimization of protein expression in a relevant platform (E. coli bacteria), focusing on overcoming insolubility, improving purification yield (titer), and utilizing fusion...
2 claims
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Gel-forming mucins. Notions from in vitro studiesThe document focuses on the challenges of producing large, complex, secretory glycoproteins (mucins) using recombinant systems, with an intense focus on Post-Translational Modifications (PTMs) such...
2 claims
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Gene regulation in continuous cultures A unified theory for bacteria and yeastsThe document develops a unified mathematical model for gene regulation and substrate consumption in continuous cultures (chemostats) of bacteria and yeasts (key expression platforms). While it is...
2 claims
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Gene targeting by TALEN-induced homologous recombination in goats directs production of β-lactoglobuAlthough the expression platform is transgenic goats (mammalian) rather than the specified microbial systems, the document addresses the production and scale of a core target protein (human...
3 claims
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Generation and Analysis of Novel Plant-Derived Antibody-Based Therapeutic Molecules against West NilThe document investigates post-translational modification (PTM) fidelity, specifically N-linked glycosylation, and its impact on the assembly and bioactivity (receptor binding, neutralization) of...
3 claims
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Generation of an engineered food-grade Lactococcus lactis strain for production of an antimicrobialThe document investigates the production and secretion of lactoferrin-derived peptides (a key target protein family in the RG) in a specific bacterial expression platform (food-grade Lactococcus...
3 claims
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Generation of diploid Pichia pastoris strains by mating and their application for recombinant proteiThe document investigates key metrics required by the research goal (titer, secretion, PTM fidelity via glycosylation analysis, strain stability, and fermentation protocols) using Pichia pastoris, a...
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Genetic Engineering of Filamentous Fungi for Efficient Protein Expression and Secretion2020 · Frontiers in Bioengineering
The document is a review focusing on genetic engineering strategies (signal peptides, UPR, etc.) to enhance heterologous protein expression, secretion, and post-translational modification (PTM...
3 claims
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Genetic Transformation of Triticeae Cereals for Molecular FarmingThe document explicitly compares the advantages and disadvantages of plant systems versus the target microbial/yeast systems (bacteria, yeasts) concerning the production of complex glycoproteins,...
3 claims
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Genetically Engineered Animals and their Intended Uses in Day to Day LifeThe document reviews the use of genetically engineered animals (biopharming) to produce complex proteins in milk for human health and pharmaceuticals (e.g., antithrombin, Rhucin), which represents a...
2 claims
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Genetically Encoded Biosensor-Based Screening for Directed Bacteriophage T4 Lysozyme Evolution2020
The document, while focusing on T4 lysozyme, uses E. coli (a target expression platform) and provides methods (directed evolution, GESS) for enhancing the bioactivity and production of a lysozyme (a...
3 claims
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Genetically modified chickens as bioreactors for protein-based drugsThe document is a review that includes a comparative analysis of different expression platforms, including microbial (bacteria) and yeast systems (Saccharomyces/Pichia), regarding production...
3 claims
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Genetically modified animals for use in biopharmacology from research to production2021
The document provides a comparative analysis of different expression platforms (including bacteria, mammalian cells, and transgenic animals) regarding critical factors specified in the research goal,...
3 claims
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Genetically modified potato plants in nutrition and prevention of diseases in humans and animals a rThe document reviews the heterologous expression and production of key target human milk proteins (human lactoferrin and human lactic β-casein, sections 3.2 and 4.2.1) in genetically modified potato...
3 claims
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Genome-Wide Pathway Analysis Reveals Different Signaling Pathways between Secreted Lactoferrin and I2013 · PLOS ONE
The research goal requires evaluating the PTM fidelity (glycosylation) and bioactivity of produced human milk proteins (HMPs), specifically Lactoferrin (LF). This document directly investigates how...
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Genome- scale metabolic model based engineering of Escherichia coli enhances recombinant single chaiThe document provides detailed strain engineering data and outcomes (GEM-guided metabolic manipulation, glk gene overexpression) applied to one of the specified platforms (bacteria/E. coli) to...
2 claims
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Genome Editing Technology and Its Application Potentials in the Industrial Filamentous Fungus Asperg2021
The document reviews advanced genome editing technologies (CRISPR/Cas9, TALENs) applied to optimize the filamentous fungus expression platform (Aspergillus oryzae) by improving heterologous protein...
2 claims
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Genome Editing with Engineered Nucleases in Economically Important Animals and Plants State of the AThe document reviews advanced genome editing techniques (CRISPR, TALENs, ZFNs) which are the essential tools used for detailed strain construction and engineering of microbial/fungal expression...
2 claims
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Genome sequence of the recombinant protein production host Pichia pastorisThe document provides the genome sequence, annotation, and codon usage for Pichia pastoris, which is one of the key expression platforms being compared in the research goal. This foundational genetic...
3 claims
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Genome sequencing and analysis of Aspergillus oryzaeThe document analyzes the genome of Aspergillus oryzae, a filamentous fungus (one of the target expression platforms) known for its ability to secrete large amounts of proteins. The findings...
2 claims
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Genotyping of recombinant Pichia pastoris strains2006
The document provides detailed strain engineering and performance data (optimization of titer/secretion, use of AOX1 promoter, alpha-factor signal sequence, and transformation methods) specifically...
3 claims
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Gensynthese, Expression und Refolding der Lipasen aus Pseudomonas species KWI 56 und ChromobacteriumProf. Dr.
Although the target proteins are lipases instead of human milk proteins, the document is a detailed PhD thesis focusing on optimizing expression, gene synthesis, refolding, and fermentation protocols...
2 claims
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Gentechnische Herstellung und Charakterisierung der Exotoxine Beta und Beta2 von Clostridium perfrinProf. Dr. Dr.
The document details the genetic production and characterization of recombinant proteins (C. perfringens toxins) using bacterial expression platforms (E. coli and B. subtilis). It addresses key...
3 claims
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Gewinnung und gentechnische Modifizierung einer rekombinanten Phospholipase A2 zur industriellen AnwProf. Dr.
The document, a PhD thesis, provides detailed methodology and results for optimizing recombinant protein expression, yield (titer), and folding/PTM fidelity (specifically disulfide bond formation via...
3 claims
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Glyco-engineered horseradish peroxidase for targeted cancer treatmentDoz. Dipl.
Although the target protein (HRP) and application (cancer treatment) are outside the scope, the document's central focus is directly on a critical research goal component: optimizing...
3 claims
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Glycosylation Status and Conformational Stability of Recombinant Stereum purpureum Endopolygalacturo2012 · Appl. Glycosci.
The document directly compares the expression and post-translational modification (PTM) fidelity (specifically disulfide bond formation and glycosylation status) of a complex protein across three of...
2 claims
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Glycosylation reduces the glycan-independent immunomodulatory effect of recombinant Orysata lectin i2021
The document compares protein production in two platforms listed in the research goal (Pichia pastoris/yeast and E. coli/bacteria) and focuses critically on how host-specific PTMs (yeast...
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Glycosyltransferases glycoengineers in human milk oligosaccharide synthesis and manufacturing2025 · Front. Mol. Biosci.
The document reviews microbial and enzymatic manufacturing methods for complex human glycans (HMOs), focusing on the use and engineering of glycosyltransferases. This knowledge regarding optimizing...
3 claims
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Grafting glycoprotein-derived oligosaccharides structures onto non-glycosylated polypeptidesGlut.
Glutaraldehyde.
The research goal focuses heavily on Post-Translational Modification (PTM) fidelity, specifically glycosylation, across different expression platforms. This document describes a novel methodology for...
2 claims
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Green Way of Biomedicine – How to Force Plants to Produce New Important ProteinsThe document, although focused on plant systems, provides a direct comparison table and discussion contrasting multiple expression platforms including Yeast and Bacteria (key systems in the research...
3 claims
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Growth of a genetically-modified Pichia pastoris and protein production in an industrial waste streaThe document focuses on optimizing the growth kinetics and maximizing titer and productivity (Qp, 125 mg/L) of a genetically modified Pichia pastoris strain (one of the target expression platforms)...
3 claims
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H11-induced immunoprotection is predominantly linked to N-glycan moieties during Haemonchus contortuFrontiers in Immunology
The document investigates why recombinant expression of a protein (H11) failed in multiple platforms (including bacteria and yeast, which are central to the research goal), concluding that the lack...
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HSF-1, HIF-1and HSP90 expression on recombinant Pichia pastoris under fed-batch fermentationThe document focuses on optimizing the fermentation conditions (temperature, methanol concentration) and characterizing stress responses (HSF-1, HSP90, HIF-1) in Pichia pastoris. Pichia is a core...
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Επίδραση των πρωτεϊνών θερμικής καταπόνησης στην έκφραση, διαλυτότητα και λειτουργικότητα της ανασυνThe document, although studying Luciferase, focuses on core biotechnological challenges highly relevant to the goal: optimizing recombinant protein expression, solubility, and functionality...
3 claims
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Harnessing Nasal Immunity with IgA to Prevent Respiratory Infections2022
The document focuses on secretory IgA (sIgA), a key target protein. Although it does not contain expression platform data (titer, secretion), it provides crucial information regarding the...
2 claims
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Harnessing evolution-guided methods for protein engineering and applications in enzyme-mediated radiThe document investigates evolutionary methods (ASR, directed evolution) to improve recombinant protein expression and yield in several target expression platforms. Chapter 3 focuses on improved...
3 claims
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Harnessing alkaline-pH regulatable promoters for efficient methanol-free expression of enzymes of in2024
The document focuses on optimizing heterologous protein expression in Komagataella phaffii (Pichia pastoris), which is one of the target expression platforms. It provides detailed strain engineering...
3 claims
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Haze Formation and Application of Insect-derived Peptidases in Wine FiningThe document is a PhD thesis which includes sections on 'Molecular cloning and recombinant expression' (1.4.1). While the subject (insect peptidases for wine fining) is unrelated, the research goal...
2 claims
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Healthy Multifunctional Spectra of Milk Glycoproteins and Their Fragments – a Review ArticleNutr. Sci.
The document is a review focusing on the biological functions, structure, and glycosylation (PTM) of Lactoferrin (LF), a key target protein. While it does not discuss heterologous expression...
2 claims
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Hemocidins in a functional and structural context of human antimicrobial peptidesFrontiers in Bioscience
The document reviews antimicrobial peptides (AMPs) and explicitly mentions Lactoferrin (a key target protein in the research goal) as a functional protein that yields bactericidal fragments. It also...
3 claims
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Heterologous expression of human ST6GAL1 in trichoderma reeseiThe document focuses on the heterologous expression of a human glycosylation enzyme (sialyltransferase ST6Gal1) in a filamentous fungus (Trichoderma reesei), which is one of the target expression...
3 claims
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Heterologous Caseins The Role of Phosphorylation in Their Functionality and How to Achieve It2025
The document is a review focusing specifically on the heterologous expression of caseins (a target human milk protein) in non-mammalian systems (bacteria, yeast, plants/fungi, matching the target...
3 claims
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Heterologous Expression of Extracellular Proteinase pAsPs of Aspergillus pseudotamarii in Komagatael2022 · Mol. Sci.
The document focuses on the heterologous expression and high-titer production of a protein in Komagataella phaffii (Pichia), which is one of the key expression platforms mentioned in the research...
2 claims
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Heterologous Expression of Recombinant Ginseng Tetradecapeptide in Saccharomyces cerevisiae and Eval2025
The document investigates the heterologous expression, yield optimization (multicopy tandem strategy/titer), and bioactivity assessment of a bioactive peptide in Saccharomyces cerevisiae. S....
3 claims
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Heterologous Expression of the Leuconostoc Bacteriocin Leucocin C in Probiotic Yeast Saccharomyces bThe document provides highly relevant strain engineering data regarding one of the specified expression platforms (Saccharomyces/S. boulardii). It details the use of a constitutive promoter (TEF1)...
3 claims
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Heterologous and efficient expression of a new alkaline pectin lyase in Pichia pastorisThe document provides detailed, high-titer production data (12.49 g/L) and specific strain engineering outcomes in a key expression platform (*Pichia pastoris*). It describes transferable methods...
3 claims
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Heterologous expression of antigenic peptides in Bacillus subtilis biofilms2016
The document investigates heterologous protein expression and engineering strategies (fusion proteins, genetic modifications, promoters) within a bacterial expression platform (*Bacillus subtilis*)....
3 claims
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Heterologous expression of human KGF FGF7 (Keratinocyte growth factor) in Pichia pastorisThe document provides detailed, empirical strain construction and expression data (using the AOX1 promoter, alpha-MF signal peptide, measured titer, and bioactivity) specifically using Pichia...
2 claims
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Heterologous expression of the thermostable cellulase CEL45A in trichoderma reeseiDoz. Dr.
The document details the heterologous expression strategy (including promoter/signal peptide usage and strain engineering) in a specific filamentous fungal host (Trichoderma reesei). Filamentous...
3 claims
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Heterologous overexpression of Glomerella cingulata FAD-dependent glucose dehydrogenase in EscherichThe document directly compares two of the target expression platforms (Pichia pastoris and Escherichia coli) regarding key metrics relevant to the research goal: expression titer/secretion efficiency...
3 claims
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Heterologous protein expression of Hydra FGFs and siRNA approach to investigate FGF functionProf.
Dr.
Although the document focuses on Hydra FGFs, it is a detailed account (PhD thesis) of heterologous protein expression challenges. It specifically compares prokaryotic (bacterial) expression failures...
2 claims
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Heterologous protein production in filamentous fungi2023
The document directly addresses one of the required expression platforms (filamentous fungi) and provides detailed information on critical factors listed in the research goal and notes, including...
3 claims
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Η πρόσδεση μορίων RNA ή η φωσφορυλίωση των RS περιοχών παρεμποδίζει την συσσωμάτωση πρωτεϊνών που πεThe document focuses on fundamental biochemical challenges in protein production, specifically how phosphorylation (a PTM) and RNA binding affect protein aggregation and solubility. Understanding how...
3 claims
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High-Level Recombinant Human Lysozyme Expressed in Milk of Transgenic Pigs Can Inhibit the Growth of2014 · PLOS ONE
Although the platform (transgenic pig milk) is not one of the targeted microbial systems, the document provides crucial benchmark data for a target protein (human lysozyme) regarding titer (116...
3 claims
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High-Level Expression of Recombinant Bovine Lactoferrin in Pichia pastoris with Antimicrobial Activi2016
The document directly addresses the production of a target human milk protein (lactoferrin) in one of the specified platforms (Pichia pastoris). It provides critical comparative data on high...
3 claims
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High-Throughput Expression of Inclusion Bodies on an Automated Platform2023
The document details high-throughput automated methods for optimizing cultivation conditions (fed-batch, screening for optimal growth rate, temperature) in Escherichia coli, which is one of the...
1 claim
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High-Throughput NMR Assessment of the Tertiary Structure of Food Allergens2012
The document details a high-throughput NMR method for assessing the tertiary structure and conformational integrity of food proteins, including cows' and goats' milk caseins (a target protein...
2 claims
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High-efficiency secretory expression and characterization of the recombinant type III human-like col2022
The document provides detailed, practical strain engineering and fermentation data (promoter engineering, high-density strategies, high titer achievement) specifically for the secretory expression of...
3 claims
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High-cell density cultivation of recombinant Pichia pastoris harboring fungal endoinulinase and prebThe document provides detailed, practical information on strain construction (vector, signal peptide, promoter), high cell density cultivation, and secretion strategies in Pichia pastoris, which is...
3 claims
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High-level expression and purification of soluble recombinant FGF21 protein by SUMO fusion in EscherThe document investigates a core production challenge (solubility and titer) in one of the required expression platforms (bacteria/E. coli). The detailed method of using the SUMO fusion tag to...
3 claims
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High-level expression of active recombinant ubiquitin carboxyl-terminal hydrolase of Drosophila melaThe document provides detailed, empirical data on optimizing expression (titer, 210 mg/L), secretion efficiency (using alpha-factor signal), and comparing strain phenotypes (Muts vs Mut+) in Pichia...
3 claims
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High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—select2008
The document is highly relevant because it studies one of the core expression platforms (Pichia pastoris) mentioned in the research goal. It provides detailed comparative data on strain selection...
3 claims
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High-level heterologous production and Functional Secretion by recombinant Pichia pastoris of the shThe document details high-level heterologous production, functional secretion, optimization strategies (mutagenesis, culture conditions), titer (418 mg/L), and challenges (plasmid loss, cell death)...
2 claims
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High-level production of animal-free recombinant transferrin from saccharomyces cerevisiaeThe document investigates high-level secretion, titer, and post-translational modification (PTM) control (specifically managing glycosylation) of recombinant transferrin (a protein functionally and...
3 claims
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High-yield expression of the recombinant, atrazine-specific Fab fragment K411B by the methylotrophicThe document focuses on detailed methodology for high-yield secretory expression (~40 mg/L titer) of a complex heterologous protein (Fab fragment) in one of the key targeted platforms, Pichia...
3 claims
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High-temperature cultivation of recombinant Pichia pastorisincreases endoplasmic reticulum stress anThe document investigates key limiting factors (titer, secretion bottleneck, ER stress, protein processing, and cell viability) affecting heterologous protein production in Pichia pastoris. Although...
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High-yield expression of the recombinant, atrazine-specific Fab fragment K411B by the methylotrophicThe document provides detailed strain construction data (AOX1 promoter, co-integration strategy), production titer (~40 mg/l), secretion data, and PTM outcomes (disulfide bond formation, discussion...
3 claims
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High Cell Density Cultivation to Produce Heterologous Proteins by S. cerevisiae Strains a Holistic AThe document is a PhD thesis focused entirely on optimizing high cell density cultivation (HCDC) and heterologous protein production (HEP) in S. cerevisiae, which is one of the key expression...
3 claims
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High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-2014 · Journal of Visualized Experiments
The document details high-throughput protocols for optimizing the expression (titer/yield) and achieving PTM fidelity (correct disulfide bond formation) of complex, disulfide-rich proteins in E....
3 claims
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High cell density fermentation strategies for the production of bovine lactoferrin in Pichia pastoriThe document focuses on optimizing high-cell density fermentation strategies and achieving high titers for lactoferrin production using Pichia pastoris, which is one of the specified expression...
2 claims
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High level expression and biochemical characterization of an alkaline serine protease from GeobacillThe document provides specific data comparing the efficiency (titer and secretion) of different expression platforms, including bacterial hosts (Bacillus subtilis, E. coli) and Pichia pastoris, for...
3 claims
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History and Promise of Plant-Made Vaccines for AnimalsAlthough the document focuses exclusively on plant expression systems and veterinary medicine (vaccines), it addresses key criteria of the research goal. It provides high-level comparative statements...
2 claims
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High level expression of bikunin in Pichia pastoris by fusion of human serum albuminAlthough the target protein (Bikunin) is not a Human Milk Protein, the document focuses extensively on high-level recombinant protein expression in one of the key target expression platforms, Pichia...
3 claims
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High throughput generation of a resource of the human secretome in mammalian cellsThe document focuses on producing the human secretome (which includes potential target proteins like lactoferrin and lysozyme) in mammalian cells (CHO, HEK). While the research goal focuses on...
3 claims
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High throughput inclusion body sizing and closed loop physiological process controlProf. Dipl.
The document focuses on high-throughput analysis and closed-loop physiological process control to optimize recombinant protein production and inclusion body (IB) formation in E. coli. E. coli is one...
2 claims
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High throughput screen for improved recombinant laccase production in Saccharomyces cerevisiaeThe document investigates detailed strain engineering strategies (gene deletions, screening methods) in Saccharomyces cerevisiae, which is one of the required expression platforms. Although the...
3 claims
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High yield vesicle packaged recombinant protein production from E. coli. (accepted)2023
The document details an innovative strain engineering method (using a VNp tag) in E. coli (a required expression platform) that significantly improves two core metrics of the research goal: secretion...
2 claims
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High‐Throughput Optimization of Recombinant Protein Production in Microfluidic Gel BeadsThe document details a high-throughput methodology for optimizing recombinant protein secretion and production titers in industrially relevant hosts, including yeast (Komagataella phaffii/Pichia...
2 claims
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Homogeneous production and characterization of recombinant N-GlcNAc-protein in Pichia pastoris2020
The document focuses on engineering a specified expression platform (Pichia pastoris) to improve Post-Translational Modification (PTM) fidelity, specifically N-glycosylation homogeneity (N-GlcNAc...
3 claims
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How Does Airway Surface Liquid Composition Vary in Different Pulmonary Diseases, and How Can We Use2024
The document discusses the composition of Airway Surface Liquid (ASL), which includes two key target proteins (lactoferrin and lysozyme) and details the characteristics of human glycosylation (e.g.,...
1 claim
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Human Cell Line-Derived Monoclonal IgA Antibodies for Cancer Immunotherapy2017
The document provides extensive and detailed information on the structure, complex glycosylation (PTM fidelity), dimerization, and bioactivity of IgA antibodies, which includes sIgA, a key target...
3 claims
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Human Lactoferrin Synergizes with Etoposide to Inhibit Lung Adenocarcinoma Cell Growth While Attenua2022
The document investigates the bioactivity (anticancer synergy) of recombinant human lactoferrin (rhLf), a target protein. Crucially, the introduction and methods discuss how the rhLf was produced...
2 claims
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Human Milk Lactoferrin and Antibodies Catalytic Activities, Complexes, and Other FeaturesThe document provides detailed structural and functional characteristics (oligomerization, complex formation, bioactivity, iron-binding capacity) of key target human milk proteins (lactoferrin, sIgA,...
2 claims
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Human Recombinant Lysosomal β-Hexosaminidase A produced in P.pastoris efficiently reduced GM2 gangliThe document provides detailed, practical methodology (Section 2.2) for high-density fermentation, induction parameters, and purification of a complex human recombinant protein (rhHex-A) using the...
2 claims
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Human basic Fibroblastic Growth Factor production by codon optimization in rice cell suspension cultAlthough the primary focus is bFGF in rice cells (not a listed platform/protein), the document provides highly transferable data on strain engineering (codon optimization, promoter/signal peptide...
2 claims
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Human milk protein production in xenografts of genetically engineered bovine mammary epithelial stem2010 · PLOS ONE
The document investigates the production and secretion of a bioactive human milk protein (beta-casein, which is listed in the research goal) using a highly specialized, alternative mammalian...
2 claims
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Human milk vs. cow-milk based infant formula proteins structure, digestion and physiological impactsFrontiers in Nutrition
The document reviews the structure, digestion, and physiological impacts of key target proteins (lactoferrin, alpha-lactalbumin, caseins) listed in the research goal. Crucially, it highlights...
1 claim
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Hydrophobins from Pleurotus ostreatus self-assembling proteins for nanobiotechnological applicationsThe document is a PhD thesis focused on the recombinant production of a protein (Hydrophobin Vmh2) using three of the exact expression platforms specified in the research goal: filamentous fungi (P....
3 claims
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Hydroxylation of recombinant human collagen type I alpha 1 in transgenic maize co-expressed with a rAlthough the document studies collagen production in transgenic maize (not a target host system), the core finding addresses a central challenge of the research goal: achieving functional...
2 claims
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Identification, heterologous expression and characterization of a dye-decolorizing peroxidase of Ple2017
The document provides specific, high-titer heterologous expression data (55,000 U L−1), signal peptide details (62 amino acids), and secretion outcomes for a protein produced in a filamentous fungus...
2 claims
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Identification, heterologous production and bioactivity of lentinulin A and dendrothelin A, two natu2021
The document utilizes Pichia pastoris, a key expression platform specified in the research goal, for the heterologous production of complex proteins. It specifically addresses critical metrics like...
2 claims
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Identification and Functional Analysis of a Lysozyme Gene from Coridius chinensis (Hemiptera Dinidor2021
The document investigates the functional analysis and recombinant expression of a lysozyme (a target protein class) using E. coli BL21 (a target bacterial expression platform). Although the protein...
2 claims
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Identification and Functional Characterization of Glycosylation of Recombinant Human Platelet-Derive2015 · PLOS ONE
The document directly addresses key aspects of the research goal, specifically the performance of the Pichia pastoris expression platform. It provides detailed functional characterization of...
3 claims
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Identification of GDF-6 blocking antibodies as anti-melanoma therapeuticsThe document, while focused on GDF6 antibodies, details specific methods for optimizing the expression and solubility of a recombinant protein in a bacterial platform (use of MBP and SlyD fusion...
1 claim
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Identification of Lysine Misincorporation at Asparagine Position in Recombinant Insulin Analogs Prod2019
The document investigates a critical product quality issue (amino acid misincorporation/fidelity error, which impacts PTM fidelity and regulatory viability) in one of the target expression platforms,...
2 claims
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Identification of lysozyme in Venetin-1 nanoparticle from the coelomic fluid of the earthworm Dendro2025 · Front. Mol. Biosci.
The document focuses on detailed structural and functional characterization (using proteomics, Cryo-EM, FTIR, Raman, and immunodetection with anti-human lysozyme antibodies) of a naturally occurring...
3 claims
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IgA and FcαRI Pathological Roles and Therapeutic Opportunities2019 · Frontiers in Immunology
The document is a review focusing on Immunoglobulin A (IgA), including secretory IgA (sIgA), which is a core target protein in the research goal. It provides detailed structural information...
3 claims
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Identification of novel factors enhancing recombinant protein production in multi-copy KomagataellaThe document investigates strain engineering strategies (multi-copy integration, helper factor co-expression) to maximize titer and secretion in Komagataella phaffii (Pichia pastoris), which is one...
3 claims
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Identifizierung und rekombinante Herstellung von Phospholipase D-Isoenzymen aus Weißkohl (Brassica oProf. Dr.
Although the target protein is plant-derived Phospholipase D rather than human milk proteins, the document provides detailed data on recombinant protein expression, cloning, optimization, inclusion...
3 claims
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Identifying promoters to enhance heterologous gene expression in recombinant Saccharomyces cerevisia2025
The document investigates optimization of heterologous gene expression using promoters (SED1P, TDH3P) in Saccharomyces cerevisiae, one of the target expression platforms. The research goal explicitly...
2 claims
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IgA tetramerization improves target breadth but not peak potency of functionality of anti-influenzaThe document focuses on characterizing the structure (tetramerization) and resultant bioactivity of recombinant secretory IgA (sIgA), which is one of the key human milk proteins listed in the...
3 claims
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Immune Response and Protective Efficacy of a Heterologous DNA-Protein Immunization with Leishmania S2017
While the target protein (Leishmania SOD) is not a human milk protein, the document details specific, transferable methodologies relevant to the research goal. It explicitly discusses expression in a...
2 claims
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Immunogenicity and antigenicity of the recombinant EMA-1 protein of Theileria equi expressed in theRev. Bras. Parasitol. Vet.
The document provides a concrete case study of recombinant protein expression in Pichia pastoris, one of the target expression platforms. it reports specific titer (389 mg/L) and confirms functional...
2 claims
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Immunogenicity evaluation of an Alum-adjuvanted recombinant prefusion RBD-Fd SARS-CoV-2 protein subuJournal of Applied Pharmaceutical Science
The document is highly relevant because it provides detailed, practical data on one of the required expression platforms (Pichia pastoris) focusing specifically on the mandated parameters: detailed...
3 claims
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Immunogenicity of a Recombinant Gonadotropin-releasing Hormone Associated to the B Subunit of EscherTechnology. Vol.
The document directly compares two expression platforms mentioned in the research goal (*Pichia pastoris* and *E. coli*). It addresses key comparison metrics such as the potential for secretion,...
2 claims
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Immunogenicity of next-generation HPV vaccines in non-human primates Measles-vectored HPV vaccine ve2016
The document directly uses Pichia pastoris, one of the key expression platforms mentioned in the research goal, to produce a recombinant protein (HPV L1). The methods for cloning, characterization,...
3 claims
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Immunogenicity of heparin-binding hemagglutinin expressed by Pichia pastoris GS115 strainJournal of Basic Medical Sciences
The document investigates expression in a key platform listed in the research goal (*Pichia pastoris* GS115). Although the target protein is HBHA (not a human milk protein), the document provides...
2 claims
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Impact of Glycosylation on Saccharomyces cerevisiae Endopolygalacturonase PGU1 Activity and StabilitThe document investigates the crucial research metrics of PTM fidelity (specifically glycosylation), activity, and stability of a recombinant protein expressed in Saccharomyces cerevisiae. Although...
3 claims
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Implications of O-glycan modifications in the hinge region of a plant-produced SARS-CoV-2-IgA antibo2024
The research goal focuses heavily on PTM fidelity (glycosylation) and bioactivity of sIgA. This document directly investigates host engineering (genome editing) to modulate O-glycosylation fidelity...
3 claims
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Implications of evolutionary engineering for growth and recombinant protein production in methanol-b2017
The document investigates strain engineering (Adaptive Laboratory Evolution) and its impact on optimizing recombinant protein production yield (titer) and growth performance in Pichia pastoris....
3 claims
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Improved Production of Recombinant Carboxylesterase FumDM by Co-Expressing Molecular Chaperones in P2023
The document investigates highly transferable strain engineering strategies (co-expression of molecular chaperones like PDI and CPR5) in a target expression platform (Pichia pastoris) to...
3 claims
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Improved Production of Recombinant Myrosinase in Pichia pastoris2021 · Mol. Sci.
The document investigates optimization strategies (signal peptide deletion, use of alpha-mating factor) for maximizing recombinant protein titer and secretion in Pichia pastoris. P. pastoris is a key...
3 claims
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Improved Refolding of a Human IgG1 Fc (CH2-CH3) Scaffold from Its Inclusion Body in <i>E. coli2022 · Biol. Pharm. Bull.
The document investigates highly relevant downstream processing methods (inclusion body solubilization and refolding, critical for yield and bioactivity) specific to the bacterial expression platform...
3 claims
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Improved heterologous protein production by a tripeptidyl peptidase gene (AosedD) disruptant of the2012 · Gen. Appl. Microbiol.
The document investigates improving the production yield (titer/secretion) of human lysozyme (HLY), which is one of the bioactive human milk proteins specified in the research goal, using the...
3 claims
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Improved production of human type II procollagen in the yeast Pichia pastoris in shake flasks by a wThe document provides detailed methodological optimization strategies (fed-batch, methanol induction protocol) for Pichia pastoris, one of the key expression platforms. It links process control to...
3 claims
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Improved production of recombinant human Fas ligand extracellular domain in Pichia pastoris yield enThe document provides detailed empirical data on optimizing production (yield, secretion, strain engineering) and handling Post-Translational Modifications (PTMs, specifically N-glycosylation) using...
2 claims
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Improved production of recombinant β-mannanase (TaMan5) in Pichia pastoris and its synergistic degra2023
The document investigates optimization strategies (signal peptide replacement, specifically comparing alpha-factor vs. a custom propeptide sequence) to improve the secretion and titer of a...
2 claims
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Improving Pharmaceutical Protein Production in Oryza sativa2013 · Mol. Sci.
The document, while focusing on plant expression systems (rice), discusses key methodological challenges central to the research goal, including optimizing titer/yield, utilizing signal peptides for...
3 claims
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Improvement of the glycoproteomic toolbox with the discovery of a unique C-terminal cleavage specifiThe document describes a novel method using the protease flavastacin to improve N-glycoproteomic analysis by specifically cleaving C-terminal to N-glycosylated asparagine. This method is explicitly...
3 claims
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Improving Algae Growth Kinetics in Suspension Bioreactors for the Production of Recombinant Proteins2016
Although the document focuses on optimizing microalgae (C. reinhardtii) as an expression platform, which is not one of the four specified platforms (Pichia, Saccharomyces, filamentous fungi,...
3 claims
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Improving recombinant eukaryotic membrane protein yields in i Pichia pastoris i The importance of coThe document investigates detailed strain engineering strategies (codon optimization, clone selection, and fusion partners) and outcomes specifically focused on maximizing protein titer in the Pichia...
3 claims
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Improving recombinant protein secretion in Pichia pastorisProf. Dipl.
The document focuses on improving recombinant protein secretion and titer in Pichia pastoris (a key expression platform listed in the research goal) using specific strain engineering techniques...
3 claims
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Improving the bioactivity of polymeric bone regenerative scaffolds through physically and chemicallyThe primary focus is bone tissue engineering, which is irrelevant. However, Chapter 3 of the dissertation explicitly reports on an analysis finding that 'the expression system used to produce the...
3 claims
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In Silico and Biochemical Characterization of Lysozyme-Like Proteins in the Rat2016 · PLOS ONE
The document provides detailed in silico and biochemical characterization (structure, disulfide bonds, bioactivity, active sites) of Lysozyme-Like proteins (LYZL), including the homolog of...
3 claims
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In Vitro Culture, Genetic Transformation and the Production of Biopharmaceuticals in Microalgae2025 · Microalgae. Int.
Although the document focuses on microalgae (not one of the four main platforms listed in the goal), it addresses all core research themes: genetic transformation, production of...
3 claims
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In vitro Impact of Yeast Expressed Hybrid Peptide CATH-2TP5 as a Prophylactic Measure Toward Sepsis2020 · Frontiers in Bioengineering
The document investigates the heterologous expression of a bioactive peptide (CATH-2TP5) in one of the required expression platforms, Pichia pastoris. Although the protein is not a human milk protein...
2 claims
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In vitro PCR verification that lysozyme inhibits nucleic acid replication and transcription2023
The document focuses entirely on Lysozyme, one of the key bioactive human milk proteins listed in the research goal. It provides detailed findings regarding its mechanism of action and bioactivity...
2 claims
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In vitro adhesion and invasion inhibition of Shigella dysenteriae, Shigella flexneri and Shigella soThe document provides critical bioactivity data (inhibition of Shigella adhesion/invasion) for several target human milk proteins (lactoferrin, sIgA, alpha-lactalbumin). Establishing the functional...
3 claims
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In vitro and in silico models for in vivo eventsProf. Dr.
The document is a PhD thesis (as requested in the notes) detailing the production of a recombinant protein (scFv) in Escherichia coli (a target expression platform). It provides highly relevant and...
3 claims
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In vitro assembly of plasmid DNA for direct cloning in Lactiplantibacillus plantarum WCSF1PLOS ONE
The research goal compares different expression platforms, including bacteria. This document details a novel and improved methodology (Gibson assembly-based direct cloning) for strain construction...
2 claims
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In vitro multi-enzymatic cascades using recombinant lysates of E. coli an emerging biocatalysis plat2020
The document discusses advancements in using recombinant E. coli lysates for biocatalysis, which relates to the utilization and engineering outcomes of one of the target expression platforms...
2 claims
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In vivo production of a novel glycoconjugate vaccine against Shigella flexneri 2a in recombinant EscThe research goal requires detailed data on titer, secretion, and PTM fidelity (glycosylation) optimization in bacterial platforms (E. coli). This document provides a highly specific, practical case...
3 claims
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Increased expression and secretion of recombinant hIFNγ through amino acid starvation-induced selectThe document investigates a specific strain engineering technique (exploiting transcriptional co-regulation of adjacent genes via selective pressure) to increase expression and secretion titer (a key...
2 claims
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Increased gene dosage for β- and κ-casein in transgenic cattle improves milk composition through comThe document focuses on producing target proteins (caseins) and analyzes metrics critical to the research goal, specifically titer, PTM complexity (sialic acid content, which is a major component of...
3 claims
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Increased information to effort ratio through physiological bioprocess developmentProf. Dr.
The document focuses on advanced physiological bioprocess development and control strategies in E. coli, a key bacterial expression platform covered by the research goal. Although specific human milk...
3 claims
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Increasing Recombinant Protein Production in E. coli by an Alternative Method to Reduce AcetateThe document details engineering strategies for the bacterial expression platform (E. coli), directly addressing the required metrics. It discusses increasing titer (acetate reduction), improving...
1 claim
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Increasing cell biomass in Saccharomyces cerevisiae increases recombinant protein yield the use of aThe document investigates strain engineering in Saccharomyces cerevisiae, a target expression platform, focusing on metabolic manipulation (respiratory strain TM6*) to significantly increase...
2 claims
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Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastorisThe document details essential strain engineering strategies (optimizing gene dosage and using qPCR for copy number determination) to maximize recombinant protein titer in Pichia pastoris. Pichia is...
3 claims
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Increasing pentose phosphate pathway flux enhances recombinant protein production in Pichia pastoris2016
The document provides specific strain engineering outcomes and detailed metabolic data for increasing recombinant protein titer in Pichia pastoris, which is one of the key expression platforms listed...
3 claims
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Inducer-free recombinant protein production in Trichoderma reesei secretory production of endogenous2023
The document investigates optimization strategies (inducer-free expression, promoter replacement, signal sequences) for high-level secretory production of heterologous proteins (nanobodies) in a...
3 claims
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Inducible promoters and functional genomic approaches for the genetic engineering of filamentous fun2015
The document details genetic engineering tools (inducible promoters, strain construction) and strain improvement strategies specifically for filamentous fungi, which is a key expression platform in...
3 claims
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Induction of the CtsR regulon improves Xylanase production in Bacillus subtilis2023
The document investigates methods (RNA-seq, genetic engineering of chaperone regulons like CtsR/Clp) to overcome secretion bottlenecks and improve titer (25% increase) in *Bacillus subtilis*. B....
1 claim
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Industrial Use of Cell Wall Degrading Enzymes The Fine Line Between Production Strategy and Economic2020 · Frontiers in Bioengineering
The document compares various expression platforms (yeast, bacteria, wild type microbes/fungi) based on critical industrial production metrics (yield, productivity, and economic feasibility/cost)....
3 claims
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Inflammatory bowel diseases what lactoferrin can do for usThe document is a detailed review focused solely on Lactoferrin (a target human milk protein) and its bioactivity (antimicrobial, immunomodulatory) and application in infant nutrition (GRAS status,...
2 claims
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Influence of Foreign DNA Introduction and Periplasmic Expression of Recombinant Human Interleukin-22013
The document investigates factors (oxidative stress, metabolic load) limiting cell growth and yield (titer) during recombinant protein production in Escherichia coli, which is one of the target...
3 claims
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Influence of cell-line and process conditions on the glycosylation of recombinant proteins2002
The document focuses heavily on PTM fidelity (glycosylation) influenced by host cell type and process conditions, a critical aspect of the research goal (bioactivity, infant nutrition). Although the...
3 claims
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Influenza A virus nucleoprotein derived from Escherichia coli or recombinant vaccinia (Tiantan) viruThe document compares the expression outcomes (titer and bioactivity/efficacy) of a recombinant protein using one of the target platforms (E. coli/bacteria) against a eukaryotic expression system...
2 claims
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Ingestion of milk containing the Dp2 peptide, a dust mite allergen, protects mice from allergic airwThe document provides detailed strain construction data, specifically using the alpha-lactalbumin promoter and alphaS1-casein signal peptide sequences to drive recombinant protein expression and...
3 claims
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Inhibition of Human MCF-7 Breast Cancer Cells and HT-29 Colon Cancer Cells by Rice-Produced Recombin2013 · PLOS ONE
Although the expression platform is transgenic rice (not microbial) and the target protein is rhIGFBP-3 (not a human milk protein), the document provides highly relevant details on strain...
3 claims
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Inhibition of invasive salmonella by orally administered IgA and IgG monoclonal antibodies2020
The document focuses heavily on the bioactivity and efficacy of orally administered Secretory IgA (SIgA) against enteric pathogens, which is one of the key human milk proteins (sIgA) and contexts...
3 claims
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Inhibition of pathogenic and spoilage bacteria by a novel biofilm-forming Lactobacillus isolate a po2015
The document investigates a specific bacterial strain (Lactobacillus plantarum) as a novel host/microbial cell factory for the expression of heterologous proteins, successfully demonstrating the...
3 claims
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Innovative Preparation of Biopharmaceuticals Using Transglycosylation Activity of Microbial EndoglycThe document focuses entirely on achieving specific, homogeneous glycosylation structures (PTM fidelity) for biopharmaceuticals using microbial endoglycosidases and sophisticated glycoengineering,...
3 claims
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Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia paThe document details advanced strain and bioprocess engineering strategies (gene dosage, PGAP promoter usage, physiological control via RQ/hypoxia) used to significantly boost recombinant protein...
3 claims
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Integrated lipase production and in situ biodiesel synthesis in a recombinant Pichia pastoris yeastThe document investigates Pichia pastoris as an expression platform, comparing it to Saccharomyces cerevisiae and others. Crucially, it provides highly transferable engineering data relevant to the...
1 claim
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Integrating metabolic modeling and population heterogeneity analysis into optimizing recombinant proThe document provides a detailed technical review of optimizing recombinant protein production in Pichia pastoris, which is one of the key expression platforms in the research goal. It focuses...
2 claims
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Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered2016
The document focuses on optimizing strain construction, integration events, and productivity (titer) in Pichia pastoris, which is one of the key expression platforms listed in the research goal. The...
3 claims
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Κλωνοποίηση και ετερόλογη έκφραση μιας κελλοβιοϋδρολάσης της οικογένειας GH7 του θερμόφιλου μύκητα SAlthough the document focuses on expressing a fungal cellulase, it uses Pichia pastoris and E. coli, which are key expression platforms mentioned in the research goal. Crucially, the abstract details...
3 claims
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Interaction Between Virus-Like Particles (VLPs) and Pattern Recognition Receptors (PRRs) From Dendri2020 · Frontiers in Immunology
The document, although focused on Virus-Like Particles (VLPs), directly discusses and compares various heterologous expression platforms (yeast, bacteria, mammalian cells) based on their capacity for...
3 claims
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Interaction of recombinant human lactoferrin and SARS-CoV-2 virus to heparin-protein conjugateThe document focuses on characterizing the bioactivity (heparin-binding affinity and structural integrity) of recombinant human lactoferrin (a target protein) using quantitative methods. Although the...
3 claims
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Interactions of Whey Proteins with Metal Ions2020
The document reviews the chemistry, structure, PTMs (including glycosylation and phosphorylation), and metal ion interactions of key target human milk proteins (alpha-lactalbumin and lactoferrin)....
2 claims
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Interactions of recombinant human lactoferrin with deoxyribonucleic acidsThe document characterizes the bioactivity (DNA binding affinity) and functional integrity of recombinant human lactoferrin (a target protein). While the source is transgenic goats, the methodology...
3 claims
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Intestinal Epithelial Barrier Function and Necrotizing Enterocolitis2022
The document discusses the biological context and functional requirement for several target human milk proteins (specifically mentioning lysozyme) by reviewing their role in preventing Necrotizing...
3 claims
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Investigating different Bacillus subtilis expression systems for recombinant enzyme productionProf. Dr.
The document investigates optimization strategies (promoters, signal peptides, 5' UTR, secretion pathways, strain engineering, titer increase) for a key expression platform (Bacillus subtilis, a...
3 claims
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Investigating the physiological response of Pichia (Komagataella) pastoris GS115 to the heterologous2013
The document directly investigates the heterologous expression and secretion of a specific bioactive human milk protein (human lysozyme) in a key target expression platform (Pichia pastoris). It...
3 claims
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Investigating xylose metabolism in recombinant Saccharomyces cerevisiae via 13C metabolic flux analyThe document investigates metabolic engineering and metabolic flux analysis (13C-MFA) in Saccharomyces cerevisiae, which is one of the key expression platforms for human milk proteins. Although the...
3 claims
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Investigation of different bioprocess modes with Escherichia coliProf. Dipl.
The document investigates different bioprocess modes (fed-batch, continuous) and process parameters for recombinant protein production in Escherichia coli (bacteria), which is one of the targeted...
3 claims
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Iron-fortified recombinant Saccharomyces cerevisiae producing Sus scrofa ferritin heavy-chain recove2012
The document investigates the production and bioactivity of a recombinant iron-related protein (Sus scrofa ferritin) using one of the target expression platforms (Saccharomyces cerevisiae). It...
3 claims
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Is there a risk from not using GE animals2009
The document, a perspective on Genetically Engineered (GE) animals, is primarily focused on regulatory viability and societal risks/benefits, which is one of the key metrics of the research goal....
2 claims
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Isolation and characterization of malaria PfHRP2 specific VNAR antibody fragments from immunized sha2018
The document investigates expression and secretion optimization within the bacterial platform (E. coli), which is one of the platforms listed in the research goal. Specifically, it details the use of...
2 claims
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Isolation of a strong constitutive promoter from Euglena gracilisThe research goal requires detailed strain construction data, including promoters, in key expression platforms (Saccharomyces and bacteria). This document focuses entirely on isolating and...
2 claims
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Isolierung des humanen Lactasegens und seine Expression in der Hefe Pichia pastorisProf. Dr.
The document is a detailed PhD thesis focusing on the expression of a complex human protein (Lactase-Phlorizin-Hydrolase) in one of the required expression platforms (*Pichia pastoris*). It contains...
3 claims
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Kinetics Study For The Recombinant Cellulosome To The Degradation Of Chlorella Cell ResidualsThe document investigates the expression and localization (secretion/anchoring) of a complex recombinant protein (cellulosome) in a bacterial expression platform (Bacillus subtilis), which is one of...
1 claim
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Kinetics Study For The Recombinant Cellulosome To The Degradation Of Chlorella Cell ResidualsThe document investigates recombinant protein expression (cellulosome) in a bacterial platform (Bacillus subtilis), which is one of the key expression platforms listed in the research goal. It...
2 claims
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Knock-in of Enhanced Green Fluorescent Protein or and Human <i>Fibroblast Growth Factor 2<Anim. Sci.
Vol.
The document investigates utilizing the porcine $\beta$-casein gene locus and its regulatory sequences to achieve high expression and secretion of heterologous proteins. Although the platform is...
3 claims
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Ko-Expression von zellulolytischen Enzymen in PflanzenzellenThe document, a PhD thesis, provides detailed methodology and results concerning the comparison of protein expression between two platforms (E. coli and eukaryotic N. tabacum), including specific...
3 claims
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Kombinierte Expression von Weizenspeicherproteinen in der Hefe Saccharomyces cerevisiaeProf. Dr.
The document investigates the heterologous expression of proteins in Saccharomyces cerevisiae, one of the target expression platforms. Its core focus is on adapting the host system and optimizing...
3 claims
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Koumiss (Fermented Mare’s Milk) as a Functional Food Bioactive Proteins, Peptides, and Future Perspe2025
The document directly addresses three of the core target bioactive human milk proteins (lactoferrin, alpha-lactalbumin, lysozyme) and analyzes their bioactivity, functional role, and concentration...
3 claims
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L-Asparaginase from Penicillium sizovae Produced by a Recombinant Komagataella phaffii Strain2022
The document compares yeast expression systems (Komagataella phaffii/Pichia and Saccharomyces cerevisiae) against bacterial systems for producing eukaryotic proteins, specifically addressing key...
3 claims
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LACTOFERIN IN THE PROBLEM OF ANTI-INFECTIOUS PROTECTION OF BABIES IN THEIR FIRST YEAR OF LIVINGThe document focuses on lactoferrin (a key target protein) and explicitly describes the biotechnology and characterization (physicochemical parameters and biological activity) of recombinant human...
3 claims
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LACTOGENIC EFFECTS OF PROBIOTIC PREPARATION ON THE BASE OF RECOMBINANTLACTOBACILLI WITH GROWTH HORMOJournal of Agriculture and Environment
The document, while focusing on GH-RF rather than Human Milk Proteins (HMPs), utilizes and details the genetic engineering of a bacterial platform (recombinant Lactobacillus sp.). The Methods section...
3 claims
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LPS ligand and culture additives improve production of monomeric MD-1 and 2 in Pichia pastoris by deThe document provides detailed strain engineering and expression optimization data for complex, disulfide-rich glycoproteins in Pichia pastoris. It specifically addresses challenges relevant to the...
3 claims
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LYZL6, an acidic, bacteriolytic, human sperm-related protein, plays a role in fertilizationPLOS ONE
The document investigates the production and bioactivity of LYZL6, which belongs to the c-type lysozyme/alpha-lactalbumin family (proteins specified in the goal). Crucially, the methodology section...
3 claims
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Label-Free Liquid Chromatography–Mass Spectrometry Quantitation of Relative N- and O-Glycan Concentr2024 · Japan. Int.
The research goal specifically focuses on PTM fidelity, particularly glycosylation, of human milk proteins (HMPs) produced in different platforms. This document provides crucial reference data by...
3 claims
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Lack of functional alpha-lactalbumin prevents involution in Cape fur seals and identifies the proteiThe document investigates the bioactivity and function of alpha-lactalbumin (LALBA), one of the target human milk proteins, specifically demonstrating its role as an apoptotic factor and proposing...
2 claims
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Lactococcus lactis As a Versatile Vehicle for Tolerogenic Immunotherapy2018 · Frontiers in Immunology
The document reviews the use of Lactococcus lactis as a robust bacterial expression platform ('biofactory') for producing and delivering bioactive proteins via mucosal delivery. Although the specific...
2 claims
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Lactic acid bacteria isolated from dairy products as potential producers of lipolytic, proteolytic a2019
The document characterizes the subcellular organization and identity of bacterial proteolytic enzymes (proteases) derived from Lactic Acid Bacteria (LAB). This provides transferable data on native...
2 claims
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Lactoferrin, a multi-functional glycoprotein Active therapeutic, drug nanocarrier & targeting ligand2020
The document is a review focusing on the therapeutic applications of Lactoferrin (Lf) rather than its recombinant production. However, it provides crucial background information on Lf's molecular...
1 claim
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Lactoferrin--a novel bone growth factor.The document focuses on the bioactivity and function of lactoferrin, a target human milk protein. Although it does not discuss expression platforms, detailed information on the biological mechanisms...
1 claim
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Lactoferrin-Conjugated Nanoparticles as New Antivirals2022
The document focuses entirely on Lactoferrin (a target human milk protein) and details its bioactivity (antiviral, immunomodulatory) and the critical role of Post-Translational Modifications...
3 claims
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Lactoferrin A Critical Player in Neonatal Host Defense2018
This review summarizes the structure, function, and bioactivity of lactoferrin (a target protein) crucial for infant defense. It provides essential context regarding the required bioactivity and...
1 claim
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Lactoferrin A Marvelous Protein in MilkAnim. Sci.
The document is a detailed review of Lactoferrin, a target protein. It extensively covers structural characteristics, particularly PTMs (Carbohydrate chains/glycosylation), and bioactivity/functional...
3 claims
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Lactoferrin A Glycoprotein Involved in Immunomodulation, Anticancer, and Antimicrobial Processes2021
The document is a detailed review focusing on the structure, function, bioactivity (immunomodulatory, antimicrobial), and the critical role of glycosylation (PTM fidelity) of lactoferrin, which is...
2 claims
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Lactoferrin A Natural Glycoprotein Involved in Iron and Inflammatory Homeostasis2017
The document is a comprehensive review focused on the bioactivity and function of Lactoferrin (a key target HMP). Understanding these functional details (iron homeostasis, inflammation,...
1 claim
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Lactoferrin A Nutraceutical with Activity against Colorectal CancerThe research goal requires evaluating the bioactivity of human milk proteins, specifically lactoferrin (Lf). This document is a detailed review focusing entirely on the bioactivity and nutraceutical...
3 claims
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Lactoferrin A Promising Therapeutic Molecule against Human Papillomavirus2024
The document focuses on Lactoferrin, one of the target proteins, detailing its structure, essential bioactivity (antiviral/immune-modulatory effects, relevant for infant nutrition), and the critical...
2 claims
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Lactoferrin A glycoprotein that plays an active role in human health2023 · Frontiers in Nutrition
This review focuses entirely on Lactoferrin (Lf), which is one of the key bioactive human milk proteins targeted by the research goal. It explicitly addresses Lf as a glycoprotein (relevant to PTM...
3 claims
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Lactoferrin Analysis of the Structure ProfileJournal of Moldova
The document provides detailed structural analysis of lactoferrin (a key target protein) and its iron-binding mechanism using X-ray crystallography data. Understanding the native structure and...
2 claims
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Lactoferrin Can Attenuate SARS-CoV-2 An Analysis of Evidential RelationsThe document is a review focusing entirely on Lactoferrin (a target human milk protein) and its bioactivity (antiviral, antibacterial, immunomodulatory). While it does not discuss recombinant...
3 claims
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Lactoferrin Decreases Enterotoxigenic Escherichia coli-Induced Fluid Secretion and Bacterial Adhesio2022
The document focuses heavily on the bioactivity of Lactoferrin (a key target human milk protein) against pathogenic E. coli, which directly addresses the 'bioactivity for infant nutrition' metric of...
3 claims
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Lactoferrin Disaggregates Pneumococcal Biofilms and Inhibits Acquisition of Resistance Through Its D2019 · Frontiers in Microbiology
The document focuses entirely on the bioactivity and functional mechanism (DNase activity, biofilm disaggregation) of one of the target human milk proteins, Lactoferrin (LF). This information is...
3 claims
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Lactoferrin Isolation Using Monolithic Column Coupled with Spectrometric or Micro-Amperometric Detec2008
The document provides detailed, optimized analytical methodologies (chromatography, spectroscopy, electrochemistry) for the isolation and quantification (titer measurement) of lactoferrin, which is...
2 claims
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Lactoferrin Properties and Potential Uses in the Food Industry2025 · Industry. Int.
The document is highly relevant to the subject matter (Lactoferrin, bioactivity, infant nutrition/food industry fortification), which is one of the target proteins and application areas of the...
2 claims
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Lactoferrin a multiple bioactive protein An overview☆2011
The document is a review focusing on Lactoferrin, a key target protein in the research goal. It explicitly mentions that recombinant Lactoferrin (rLf) is generated from bacterial and fungal/viral...
3 claims
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Lactoferrin and lysozyme to reduce environmental enteric dysfunction and stunting in Malawian childrThe document, a clinical trial protocol, focuses entirely on assessing the bioactivity and clinical efficacy of two key proteins mentioned in the research goal (lactoferrin and lysozyme) for infant...
3 claims
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Lactoferrin and Its Role in Neonatology A Review ArticleThe document reviews the function, bioactivity, and clinical/regulatory relevance of Lactoferrin (a key target protein) in neonatology and infant nutrition. It specifically mentions a clinical trial...
3 claims
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Lactoferrin for Prevention and Treatment of Anemia and Inflammation in Pregnant Women A Comprehensiv2021
The document is a comprehensive review detailing the bioactivity, functional mechanism (iron regulation, anti-inflammatory), and clinical application of lactoferrin (one of the key target human milk...
1 claim
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Lactoferrin in a Context of Inflammation-Induced Pathology2017 · Frontiers in Immunology
The document is a detailed review of lactoferrin (a target protein) function and bioactivity. Crucially, it explicitly discusses how specific glycosylation patterns (PTMs, including N-linked sites...
2 claims
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Lactoferrin in the Battle against Intestinal Parasites A ReviewThe document provides detailed information on the bioactivity, structure, and functional domains (Lactoferricin) of lactoferrin, which is one of the target proteins. This context is essential for...
2 claims
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Lactoferrin – A Potential Anabolic Intervention in OsteoporosisThe document provides detailed structural information (including glycosylation) and functional definitions (bioactivity: antimicrobial, immunomodulatory, iron binding) for Lactoferrin, which is a...
3 claims
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Lactoferrin inhibits E. coli O157 H7 growth and attachment to intestinal epithelial cellsThe document provides detailed findings regarding the bioactivity (anti-microbial efficacy and mechanism of action against E. coli O157:H7 attachment) of a core target human milk protein...
3 claims
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Lactoferrin is a dynamic protein in human melioidosis and is a TLR4-dependent driver of TNF-α releasThe document investigates the bioactivity and function of human lactoferrin (a target protein) in an immune context, including measurements using purified and recombinant human lactoferrin. Although...
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Lactoperoxidase Properties, Functions, and Potential Applications2025 · Applications.
Int.
The document reviews Lactoperoxidase (LPO), a key functional enzyme found in milk, which is highly adjacent to the list of target Human Milk Proteins (HMPs) in the research goal (lactoferrin,...
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Lactoferrin’s Anti-Cancer Properties Safety, Selectivity, and Wide Range of Action2020
The document is a review focusing on the bioactivity (specifically anti-cancer properties) and structural aspects (iron binding, PTMs/glycosylation) of Lactoferrin, which is one of the key human milk...
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Large-Scale Production in 'Escherichia coli' TG1 and Purification of Llama Single Domain Antibody ToThe document provides detailed, high-level data from a PhD thesis on optimizing high-cell-density culture (HCDC), induction parameters, titer (127 mg/L), and specialized downstream processing...
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Large-scale production of functional human lysozyme from marker-free transgenic cloned cowsThe document focuses on producing and characterizing a target human milk protein (lysozyme) for infant nutrition, providing data on titer (3149 mg/L) and confirmed enzymatic bioactivity. Although the...
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Large-scale production of recombinant human lactoferrin from high-expression, marker-free transgenicThe document focuses on producing a target human milk protein (lactoferrin) and provides crucial comparative data on titer (4.5–13.6 g/L) and confirmed bioactivity (iron binding/release). While the...
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Learning about protein solubility from bacterial inclusion bodiesThe document discusses methods and findings related to protein quality, solubility, folding, bioactivity, and yield optimization specifically in 'recombinant bacteria,' which is one of the key...
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Lichtbasierte Steuerung und Analyse der Produktion von rekombinanten Proteinen in Kultivierungen imProf. Dr.
The document details advanced high-throughput methodologies (BioLector coupled with light-mediated control/induction) for optimizing the production of recombinant proteins in key expression platforms...
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Long-Lasting Stable Expression of Human LL-37 Antimicrobial Peptide in Transgenic Barley Plants2021
The document investigates the heterologous expression, quantification (titer), and confirmed bioactivity of a human antimicrobial peptide (LL-37). While the specific platform (Barley/plant) and...
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Lower Temperature Cultures Enlarge the Effects of Vitreoscilla Hemoglobin Expression on Recombinant2012 · Mol. Sci.
The document focuses on optimizing recombinant protein production (titer/yield) in Pichia pastoris, which is one of the key expression platforms mentioned in the research goal. It details specific...
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Lysozyme improves gut performance and protects against enterotoxigenic Escherichia coli infection in2018
The document investigates the bioactivity and efficacy of recombinant human lysozyme (a target protein) in a relevant neonatal context (piglets consuming hLZ milk), directly addressing the...
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MOSAIC Codon Harmonization of Monte Carlo-Based Simulated Annealing for Linked Codons in HeterologouThe document details an advanced method (codon harmonization/MOSAIC algorithm) for strain engineering aimed at significantly increasing the titer and improving the soluble expression and functional...
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Magnetic Immobilization of Pichia pastoris Cells for the Production of Recombinant Human Serum Album2020
The document optimizes the Pichia pastoris expression platform (a target system) using novel immobilization techniques, which significantly increases the titer/yield of a recombinant protein (HSA)...
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Make it double Identification and characterization of a Tandem-Hirudin from the Asian medicinal leecThe document, while focusing on Hirudin, provides detailed, transferable expression and downstream processing methodology for complex, disulfide-rich foreign proteins in the bacterial platform (E....
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Making recombinant proteins in filamentous fungi- are we expecting too much2014
The document is a review focusing specifically on one of the required expression platforms (filamentous fungi) and addresses key comparison metrics central to the research goal, including protein...
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Mammalian Glycosylation Patterns Protect Citrullinated Chemokine MCP-1 CCL2 from Partial Degradation2023 · Degradation. Int.
The document investigates how post-translational modification (PTM) fidelity, specifically mammalian glycosylation patterns, protects recombinant proteins from degradation. It directly contrasts the...
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Mammalian Glycosylation Patterns Protect Citrullinated Chemokine MCP-1 CCL2 from Partial Degradation2023 · Degradation. Int.
The document directly compares the outcomes of protein production (stability, degradation, and PTM fidelity, specifically glycosylation) in key expression platforms listed in the research goal,...
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Mammalian cell lines used in bioprocessing2022 · Journal of Experimental and Clinical Medicine
The document is a review that directly compares the primary expression platforms of interest (Pichia, Saccharomyces, bacteria) concerning key metrics like growth rate, yield, secretion, and, most...
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Matrix-immobilized yeast for large-scale production of recombinant human lactoferrinMed. Chem. Commun.
The document directly addresses the research goal by studying the production and purification of a target human milk protein (lactoferrin) using a target expression platform (Saccharomyces...
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Mechanism-guided control of intracellular alkalinization and process pH for production of recombinanThe document details process optimization, medium engineering, and mechanism-guided pH control in the Escherichia coli (bacterial) expression platform to maximize the titer and stability of a...
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Mechanism of cooperative N-glycan processing by the multi-modular endoglycosidase EndoE2022
The document investigates a bacterial endoglycosidase (EndoE) that processes N-glycans. The research goal is highly focused on PTM fidelity (glycosylation) in various expression platforms (including...
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Mechanisms Affecting the Gut of Preterm Infants in Enteral Feeding Trials.2017 · Frontiers in Nutrition
The document describes clinical trials (ELFIN/MAGPIE) focused on the enteral use and mechanisms of action of supplemental lactoferrin (a target human milk protein) in preterm infants. While it lacks...
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Mechanistic platform knowledge of concomitant sugar uptake in Escherichia coli and analysis thereofIng. Dr.
The document is a PhD/Master thesis detailing mechanistic platform knowledge for optimizing recombinant protein production (titer, solubility, induction strategy) in Escherichia coli BL21(DE3) using...
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Mechanistic studies on human pancreatic [alpha]-amylaseThe document is a PhD thesis containing detailed experimental data on expressing a recombinant human protein (HPA) in one of the target expression platforms (Pichia pastoris). It provides concrete...
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Medical Biotechnology Problems and Prospects in BangladeshThe document provides a general overview of using microbial platforms (bacteria and yeast) for the production of recombinant human proteins and mentions regulatory concepts (FDA approval), which are...
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Metabolic Engineering von mikrobiellen Wirten zur Biosynthese von hochmolekularem HyaluronanThe document is a PhD thesis focusing on metabolic engineering and chassis construction using key target expression platforms: Saccharomyces cerevisiae and bacteria (Lactococcus lactis). Although the...
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Metabolic engineering and characterisation of the malolactice wine yeast ML01The document describes detailed metabolic engineering of the required expression platform (Saccharomyces cerevisiae), including strain construction methods (PGK1 promoter, URA3 locus integration),...
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Metabolic engineering of Kluyveromyces lactis for L-ascorbic acid (vitamin C) biosynthesisThe document focuses on detailed strain construction data (promoters: LAC4, GPD1, ADH1; genomic integration) and heterologous expression outcomes in Kluyveromyces lactis. K. lactis is a key...
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Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose methanoThe document investigates metabolic flux distribution and metabolic burden in Pichia pastoris during recombinant protein secretion, focusing on improving production efficiency (titer and secretion)....
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Metabolic modeling to identify engineering targets for Komagataella phaffii The effect of biomass coThe document focuses on developing a genome-scale metabolic model for Komagataella phaffii (Pichia pastoris), one of the target expression platforms, specifically to identify genetic engineering...
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Metabolomic analysis of riboswitch containing E. coli recombinant expression systemThe document investigates the metabolic burden and regulatory control mechanisms (riboswitches, dual transcription-translation control) for recombinant protein production in E. coli, a key platform...
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Methods for producing soluble, biologically-active disulfide-bond containing eukaryotic proteins inAppl. No.
The document is a patent detailing methods for producing soluble, biologically active, disulfide bond-containing eukaryotic proteins in bacterial hosts (E. coli). This directly addresses the research...
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Method development and its application for N-glycan analysis of influenza A virus antigensProf. Dr.
The core focus of the research goal is comparing PTM fidelity (glycosylation) across different expression platforms. While the document analyzes viral proteins expressed in mammalian cells (MDCK), it...
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Methyl-selective isotope labeling using α-ketoisovalerate for the yeast Pichia pastoris recombinantThe document provides specific data on strain construction (X33 strain, pPIZα vector) and fermentation optimization (titer selection, pH conditions, methanol consumption) for producing a secreted...
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Microarray-Based Transcriptome Analysis of Recombinant Pichia Pastoris Strains Overexpressing Alpha-Prof. Dr.
The document investigates optimization strategies and strain engineering details (vector system pPIC9, AOX1 promoter, alpha-factor secretion signal) in Pichia pastoris, one of the required expression...
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Microarray-based evaluation of selected recombinant timothy grass allergens expressed in E. Coli and2024
The document compares bacterial (E. coli) and eukaryotic (N. benthamiana) expression platforms for producing recombinant proteins. Crucially, it analyzes how the platform choice impacts protein...
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Milk-Derived Antimicrobial Peptides Overview, Applications, and Future PerspectivesThe document provides the regulatory framework (FSSAI standards in India) governing 'infant milk food,' 'casein,' and 'whey proteins.' This directly addresses the 'regulatory viability' component and...
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Milk Bioactive Compounds and Gut Microbiota Modulation The Role of Whey Proteins and Milk Oligosacch2024
Reviews the bioactivity and function of key human milk proteins (lactoferrin, alpha-lactalbumin, lysozyme) regarding gut microbiota modulation and infant nutrition. This information is essential for...
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Milk Derived Peptides with Immune Stimulating Antiviral PropertiesThe document discusses the antiviral and immune-stimulating bioactivity of the exact target proteins (lactoferrin, caseins, alpha-lactalbumin) central to the research goal. This provides essential...
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Model-based strategies for scale-down studies in fed-batch cultivation of Escherichia coli expressinDr. Ing.
The document investigates critical bioprocess engineering challenges (scale-up effects, yield/titer reduction, and quality/PTM fidelity issues via non-canonical amino acid misincorporation) in the...
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Modeling, analysis, and design of complex biological networksCurvat. Ms.
The document is a PhD thesis focused on computational systems biology and modeling/design of biological networks. Crucially, the abstract explicitly states the reconstruction and application of...
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Modeling and measuring intracellular fluxes of secreted recombinant protein in Pichia pastoris withThe document investigates intracellular fluxes, secretion, degradation, and titer optimization strategies (strain improvement via overcoming bottlenecks like ERAD/UPR) in Pichia pastoris. Pichia is a...
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Modellierung der Adsorption von Proteinen an OberflächenProf. Dr.
This PhD thesis models the physical behavior and adsorption characteristics of target human milk proteins, specifically Lysozyme (4.4.1, 5.2), and related complex proteins (Antibodies/IgG1, relevant...
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Modern Approaches in Probiotics Research to Control Foodborne PathogensThe research goal focuses on expression platforms (Saccharomyces, bacteria) and bioactivity for infant nutrition. This document focuses on 'Modern Approaches in Probiotics Research,' including...
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Modelling concentration gradients in fed‐batch cultivations of E. coli – towards the flexible design2018 · Journal of Chemical Technology
The document investigates bioprocess scale-down modeling in E. coli (a target expression platform) to assess the impact of nutrient gradients on recombinant protein quality (non-canonical amino acid...
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Modulating Oral Delivery and Gastrointestinal Kinetics of Recombinant Proteins via Engineered Fungi2021
The document focuses on engineering Saccharomyces cerevisiae (a target platform) for recombinant protein secretion and delivery. It explicitly addresses key research metrics such as achieving high...
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Modulation of Kex2p Cleavage Site for In Vitro Processing of Recombinant Proteins Produced by i SaccMicrobiol. Biotechnol.
The document focuses on engineering the secretory pathway (Kex2p processing, signal peptides, cleavage sites) in Saccharomyces cerevisiae, one of the target expression platforms. This detailed strain...
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Molecular Approaches for Diagnosis, Therapy and Prevention of Cow’s Milk Allergy2019
The document reviews the use of recombinant milk proteins (alpha-lactalbumin, lactoferrin, caseins—homologs of the target human proteins) for diagnostic and therapeutic purposes. Although the context...
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Molecular Cloning, Expression and Purification of Recombinant VHH Proteins Expressed in &lt;i&am2021 · Journal of Molecular Biology
The document details molecular cloning, strain construction (promoters, PelB signal peptide for secretion), and yield metrics for recombinant protein expression in E. coli, which is one of the...
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Molecular Cloning and Characterization of a New C-type Lysozyme Gene from Yak Mammary TissueAnim. Sci.
Vol.
The document directly addresses the research goal by comparing the production of a target human milk protein, lysozyme, in two key expression platforms: Escherichia coli (bacteria) and Pichia...
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Molecular Cloning and Expression of Recombinant Allium Sativum Root Lectin in Pichia Pastoris X-33Journal of Biomedical
The document investigates a core component of the research goal: heterologous protein expression in the Pichia pastoris platform. It provides detailed, transferable data regarding strain construction...
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Molecular Cloning and Recombinant Protein Expression of Hypoallergen Der p 2 1S in Saccharomyces bou2025
The document investigates recombinant protein expression using one of the required platforms (*Saccharomyces*). It provides highly relevant technical details on strain construction, including the use...
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Molecular adaptation to high temperatures pernisine from the archaeon Aeropyrum pernix K11990
Although the specific protein studied is an archaeal protease (pernisine), the document is a review summarizing the strategies for recombinant protein production using the exact expression platforms...
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Molecular basis of sugar recognition by collectin-K1 and the effects of mutations associated with 3M2015
The document investigates the molecular basis of protein secretion failure due to structural defects (Ca2+ binding issues), which provides transferable knowledge for strain engineering efforts aimed...
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Molecular biological and biochemical investigation of 2-oxoglutarate dependent dioxygenases in Equis2011 · Prof. Dr. Dr.
Although the target molecules are plant enzymes (flavonoid dioxygenases) and not human milk proteins, this PhD thesis details molecular biology, cloning, purification, and heterologous expression...
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Molecular cloning and isolation of a recombinant alpha-Momorcharin in E. coli against Pyricularia orThe document focuses on cloning, optimizing, expressing, and purifying a recombinant protein (Alpha-Momorcharin) in a bacterial expression platform (E. coli), which is one of the platforms required...
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Molecular genetic and biochemical interaction studies of the transcriptional activator Gal4 and itsProf. Dr.
The document provides detailed molecular genetic and biochemical studies of the GAL/LAC regulatory switch (Gal4/Gal80) in Saccharomyces cerevisiae and Kluyveromyces lactis. The GAL system is a...
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Molecular genetic approaches to decrease the uncontrolled misincorporation of non-canonical branched2022
The document addresses critical strain engineering challenges in a target expression platform (bacteria/E. coli) focusing on post-translational modification (PTM) fidelity—specifically, reducing...
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Molekularbiologische und biochemische Untersuchungen zu Prenyltransferasen und NRPS-ähnlichen EnzymeProf. Dr.
The document, a PhD thesis on fungal enzyme investigation, provides detailed methodologies for molecular biology, genetic manipulation, and heterologous expression (Section 2.5.12) within Ascomycetes...
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Molekularbiologische Untersuchungen zur Bedeutung von Kallikreinen und Serinproteinase-Inhibitoren fProf. Dr.
The document investigates the expression and functional characterization of recombinant proteins (Kallikreins) in a key target expression platform specified in the goal (*Pichia methanolica*)....
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Molekulare Charakterisierung von c-Typ- und i-Typ-Lysozymen des Asiatischen Marienkäfers Harmonia axThe research goal involves comparing expression platforms (Pichia/Saccharomyces, bacteria) for producing human milk proteins, specifically lysozyme. This document, a PhD thesis, details the molecular...
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Monokaryotic pleurotus sapidus strains with intraspecific variability of an alkene cleaving dyp-type2021 · Expression. Int.
The document investigates factors (gene mutation, differential gene expression) affecting enzyme production, activity, and stability within a fungal system (*Pleurotus*) and uses one of the target...
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Multifunctional Iron Bound Lactoferrin and Nanomedicinal Approaches to Enhance Its Bioactive Functio2015
The document focuses extensively on the structure, function, and bioactivity of Lactoferrin, a key target protein. This information is crucial for establishing the required PTM fidelity and...
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Multimodal Approaches for the Improvement of the Cellular Folding of a Recombinant Iron Regulatory PThe document focuses on optimizing the productivity, solubility, and functional activity of a recombinant protein using detailed strain engineering and physiological modulation (chaperones,...
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Multimodal approaches for the improvement of the cellular folding of a recombinant iron regulatory p2022
The document investigates detailed strain engineering and optimization strategies (chaperone co-expression, stress response modulation, process conditions) in E. coli to enhance the folding,...
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Multiple knockout genes encoding proteases to increase the recombinant protein content in NicotianaThe document describes detailed strain engineering methodology (CRISPR-mediated multiple protease knockout) specifically aimed at increasing recombinant protein titer/accumulation by reducing...
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Multiple strategies were adopted to optimize the enzymatic characteristics and improve the expressioFrontiers in Microbiology
Although the document focuses on bovine chymosin (not a human milk protein), it provides highly relevant and detailed methodological data concerning strain engineering optimization in a yeast...
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N-glycosylation site occupancy in human prostaglandin H synthases expressed in Pichia pastorisThe document investigates key engineering challenges (N-glycosylation site occupancy, protein folding, activity, signal peptides, codon optimization) for expressing a complex human glycoprotein in...
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N-hypermannose glycosylation disruption enhances recombinant protein production by regulating secretThe document investigates glycosylation engineering (deletion of OCH1, MNN9) in Saccharomyces cerevisiae, a target expression platform, specifically addressing PTM fidelity (hypermannosylation) and...
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NOVEL PROCESSES AND PRODUCTS FOR RECOMBINANT PRODUCTION OF BIOPHARMACEUTICALSThe document performs a comparison of recombinant protein production (antibody fragments) across microbial hosts including bacteria, yeasts, and filamentous fungi, directly addressing the platform...
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Native-State Stability Determines the Extent of Degradation Relative to Secretion of Protein Variant2011 · PLOS ONE
The document directly studies the production and secretion optimization of human lysozyme (a key target human milk protein) in Pichia pastoris (a key expression platform). It provides detailed...
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Native und rekombinante Pyruvatdecarboxylase aus Pisum sativum - Gemeinsamkeiten und Unterschiede inProf. Dr.
This detailed PhD thesis serves as a case study for heterologous protein expression, using two of the target platforms (*Saccharomyces cerevisiae* and *Escherichia coli*). While the target protein...
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Natural Antimicrobial as an Alternative Food Preservatives in the Dietary Systems of Human and LivesThe document, while focusing on food preservation applications, explicitly discusses the antimicrobial bioactivity and sources of two key target proteins (lactoferrin and lysozyme), providing crucial...
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Natural Glycoforms of Human Interleukin 6 show atypical plasma clearanceThe document investigates the critical relationship between specific N-glycan structures (PTM fidelity) and the resulting in vivo biological function (plasma clearance/PK) and bioactivity of a...
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Natürlich vorkommende Autoantikörper – Charakterisierung, Klonierung und Testung rekombinanter Antik2019 · Prof. Dr.
The document details the cloning, production, and testing of recombinant antibodies (scFv-Fc). Crucially, it specifically investigates the 'Veränderung des Fc N-Glykosylierungsmusters' (change of the...
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Natürliche Kompetenz und Proteinexport in Bacillus megaterium – Grundlagen und biotechnologische Anw2013 · Prof. Dr.
The document focuses on recombinant protein export and secretion mechanisms (SEC-Pathway) in the bacterial expression platform Bacillus megaterium. This directly addresses the research goal's...
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Necrotizing enterocolitis in premature infants—A defect in the brakes Evidence from clinical and aniThe document discusses the pathogenesis of Necrotizing Enterocolitis (NEC) in premature infants, focusing on Toll-like receptor (TLR) signaling defects. This information is crucial for defining and...
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Neuroprotective Role of Lactoferrin during Early Brain Development and Injury through Lifespan2022
The document provides critical context regarding the bioactivity, function (neuroprotection, anti-inflammatory), and nutritional importance of Lactoferrin (a core target protein). This information is...
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New biosystems from applied research to technology transferThe research goal focuses on comparing expression platforms (Pichia, bacteria, fungi) for heterologous protein production. This document, particularly Chapters 2 and 3, details the recombinant...
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New immunoassay systems based on recombinant human lactoferrinThe document describes the development of specific and precise immunoassays (ELISA, TR-LIFMA) designed to measure the concentration and biological activity of recombinant human lactoferrin (rhLF)....
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New oxidative enzimes for industrial applicationThe document details strain engineering and optimization strategies (EP-PCR, rational design) for heterologous protein expression (laccase) in a key platform specified in the goal (filamentous fungi,...
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Next Generation of Transgenic Plants From Farming to PharmingThe document, particularly Table 1, provides a direct comparison of expression platforms, including Bacteria and Yeast (Saccharomyces/Pichia), using key metrics central to the research goal, such as...
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Novel Insights in the Application of Probiotic Yeast Saccharomyces boulardii in Dairy Products and H2024
The document discusses Saccharomyces boulardii, a key yeast expression platform mentioned in the goal. Specifically, it reviews the use of genetic engineering/metabolic engineering in S. boulardii...
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Novel approach of high cell density recombinant bioprocess development Optimisation and scale-up froThe document investigates high-throughput bioprocess development, vector optimization (promoters, fusion partners), and scale-up strategies to maximize recombinant protein titer in E. coli (a...
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Nucleic Acids In Inclusion Bodies Obtained From E. Coli Cells Expressing Human Interferon-GammaThe document focuses on detailed methods for purification and analysis of inclusion bodies (IBs) during recombinant protein production in E. coli (a bacteria expression platform mentioned in the...
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OPENPichia licence-free Komagataella phaffii chassis strains and toolkit for protein expression2024
The document focuses entirely on developing and improving the Pichia pastoris (Komagataella phaffii) expression platform, which is one of the key expression systems listed in the research goal. It...
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OPTIMIZING THE PRODUCTION OF A FUNCTIONAL TYPE A RECOMBINANT ENDOCHITINASE FROM Trichoderma asperellJournal of Experimental Biology and Agricultural Sciences
The document optimizes high-titer, soluble production of a recombinant enzyme in E. coli (a target platform). While the specific protein (chitinase) and application (agriculture) are incorrect, the...
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Obtaining practically valuable compounds using recombinant yeast Saccharomyces cerevisiae. Part 2 syThe document is a review focusing on using recombinant Saccharomyces cerevisiae for expressing and secreting heterologous proteins, noting its use for 'biologically active substances' and discussing...
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Occurrence, structure, biochemical properties and technological characteristics of lactoferrinThe document is a detailed review of lactoferrin (a target human milk protein) focusing on its structure, PTMs (N-glycans), and biochemical properties/bioactivity. This foundational knowledge is...
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Optimizing the glycosylation of recombinant proteinsProf. Dr.
The document focuses entirely on optimizing and characterizing the glycosylation of recombinant proteins, which is the key PTM fidelity metric required by the research goal. Although the specific...
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On the Role of Aggregation Prone Regions in Protein Evolution, Stability, and Enzymatic Catalysis In2013
The document investigates aggregation prone regions (APRs) and their role in protein stability and solubility, explicitly stating the findings are relevant for rational design strategies aimed at...
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Opioid peptides encrypted in intact milk protein sequencesThe document defines the key structural features and functional significance (bioactivity) of specific peptides derived from the target human milk proteins (caseins, lactoferrin, alpha-lactalbumin)....
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Optimisation of active recombinant protein production, exploring the impact of small heat-shock protProf. Dr.
The document focuses on optimizing the production, folding, and yield (titer) of active recombinant protein in Escherichia coli, which is one of the expression platforms ('bacteria') central to the...
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Optimisation of Recombinant Myrosinase Production in Pichia pastoris2021 · Mol. Sci.
The document provides detailed optimization data (titer, secretion, upscaling, high-cell density fermentation, and strain engineering outcomes) for Pichia pastoris, which is one of the key expression...
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Optimising expression and extraction of recombinant proteins in plants2022 · Frontiers in Plant Science
While the document focuses on plant expression systems, which are not listed in the research goal, it extensively discusses core transferable concepts critical to the goal: optimizing recombinant...
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Optimization and improvement of recombinant chitinase biosynthesis in E. coli using response surfaceThe document investigates optimizing expression conditions (titer, induction time, cell density, alternative inducers like lactose) for a recombinant protein in E. coli, which is one of the key...
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Optimization and scale up production of recombinant goldfish hormone in pichia pastoriaThe document provides detailed, practical engineering and scale-up data for one of the target expression platforms (Pichia pastoris), covering optimization strategies, fermentation scale-up (14L),...
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Optimization of Dengue-3 recombinant NS1 protein expression in E. coli and in vitro refolding for di2012
The document details methods for optimizing protein expression, yield (titer), and achieving proper folding/bioactivity (in vitro refolding) in the bacterial expression system (E. coli). This...
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Optimization of Expression, Purification and Secretion of Functional Recombinant Human Growth HormonAlthough the target protein (hGH) is not a human milk protein, the document directly addresses core technical components of the research goal using one of the required expression platforms...
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Organismos geneticamente modificadosArch. Zootec.
This is a broad review on Genetically Modified Organisms (GMOs) and recombinant DNA technology. While the primary focus is on transgenic crops and animals, it specifically mentions the expression of...
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Optimization of Expression and Purification of Recombinant One of Central Nervous System Enzyme (Ace2024 · Journal of Science and Engineering
The document, while focusing on Acetylcholinesterase (AChE) instead of human milk proteins (HMPs), provides direct, practical methodology for optimizing expression yield, purification, and secretion...
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Optimization of Fermentation Medium for the Production of Recombinant Human Antithrombin III from SaJournal of Applied Pharmaceutical Science Vol
The document optimizes recombinant protein titer (rhAT) using statistical experimental design (DoE/RSM) in Saccharomyces cerevisiae. Although the target protein is different, the platform...
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Optimization of a Recombinant Lectin Production in Pichia pastoris Using Crude Glycerol in a Fed-Bat2021 · Queretaro.
Av.
The document investigates optimization strategies (fed-batch, use of crude glycerol, process parameters) to improve titer and secretion efficiency in one of the primary expression platforms listed in...
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Optimization of expression, purification and secretion of functional recombinant human growth hormonThe document details the engineering of a signal peptide and optimization of the secretion pathway (Sec pathway) to achieve high-yield, functional secretion of a recombinant human protein (hGH) in a...
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Optimization of human chaperone BiP production in yeast Pichia pastorisThe document provides detailed optimization data for high-yield protein secretion (titer, BSM medium, fed-batch strategies, use of native signal peptides, and handling PTMs/folding via reducing...
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Optimized Production and Analysis of Recombinant Protein-Filled Vesicles from <em>E. coli<2023 · Vis. Exp.
The research goal requires comparing titer, secretion, and folding (PTM fidelity, e.g., disulfide bonds) across different platforms, including bacteria (E. coli). This document details an engineered...
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Optimized transgene expression in the red alga Porphyridium purpureum and efficient recombinant prot2024
Although the document focuses on a different host (red microalga Porphyridium) than the platforms specified in the goal (yeast, fungi, bacteria), it provides detailed, transferable technical findings...
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Optimizing Scaled up Production and Purification of Recombinant Hydrophobin HFBI in Pichia pastoris2025
The document provides detailed, transferable data concerning the comparison of relevant expression platforms (Pichia, E. coli, filamentous fungi) regarding titer optimization, secretion, strain...
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Optimizing cofactor availability for the production of recombinant heme peroxidase in Pichia pastoriThe document provides detailed strain engineering data (promoter selection, metabolic pathway modification, and medium optimization strategies) specifically for recombinant protein production in...
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Oral Administration of Recombinant Saccharomyces boulardii Expressing Ovalbumin-CPE Fusion Protein I2018 · Frontiers in Microbiology
The document provides detailed strain engineering outcomes and construction data (using the alpha-mating factor secretion signal, inducible promoter systems, and CRISPR/Cas9) for the Saccharomyces...
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Oral Vaccination With Recombinant Pichia pastoris Expressing Iridovirus Major Capsid Protein ElicitsFrontiers in Immunology
The document investigates protein expression using Pichia pastoris (a key platform listed in the research goal). It provides highly relevant and transferable technical details on strain construction...
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Osteogenic activity of lactoferrin and its application in contemporary dentistryThe document focuses on the bioactivity (osteogenic activity) of Lactoferrin, one of the target human milk proteins. Since the research goal emphasizes assessing bioactivity and PTM fidelity,...
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Over-expression in Escherichia coli of the ToxA51 llama single domain antibody targeting ClostridiumThe document investigates the over-expression of a recombinant protein (single domain antibody) in E. coli (a required bacterial platform). It provides detailed data on titer (503 mg/L), optimization...
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Overexpression of a Fungal β-Mannanase from Bispora sp. MEY-1 in Maize Seeds and Enzyme Characteriza2013 · PLOS ONE
The document, while focusing on expressing a fungal enzyme in maize, includes comparative characterization data for the same enzyme expressed in Pichia pastoris. Pichia pastoris is one of the core...
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Overexpression of a Rhizopus Oryzae Lipase in Pichia Pastoris Strains Containing Multiple Copies ofThe document provides highly specific, transferable strain engineering and optimization data for one of the key expression platforms (Pichia pastoris) relevant to the goal. It details methods for...
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Overexpression of an antimicrobial peptide derived from C. elegans using an aggregation-prone proteiThe document investigates a bacterial expression strategy (E. coli inclusion body formation) which is one of the platforms specified in the research goal. Crucially, the study uses human...
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Overexpression of human virus surface glycoprotein precursors induces cytosolic unfolded protein resThe document investigates major expression bottlenecks (translocation inefficiency, aggregation, lack of glycosylation, and resulting host stress response/UPR) when producing complex mammalian...
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Overproduction, characterization and preliminary antiproliferative activity determination of non-tag2017 · Indonesia. Tel.
The document details the engineering, overproduction, and characterization of a recombinant protein using Pichia pastoris, which is one of the key expression platforms specified in the research goal....
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Oxidative protein folding investigating new enzymes, new assays, and new locationsThe document investigates oxidative protein folding, focusing on enzymes (PDI, Ero1p, Erv2p, QSOX) and assays crucial for forming correct disulfide bonds (a critical PTM) in secreted proteins....
2 claims
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PAOX1 expression in mixed-substrate continuous cultures of Komagataella phaffii (Pichia pastoris) is2023 · Frontiers in Bioengineering
The document investigates the specific expression rate (titer/productivity) driven by the key industrial promoter P_AOX1 in Komagataella phaffii (Pichia pastoris), which is one of the primary...
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PECULIARITIES OF PHYSIOLOGICAL AND BIOCHEMICAL BLOOD STATUS AND PRODUCTIVITY OF CALVES IN THE POSTNAThe document investigates the bioactivity and efficacy of recombinant lactoferrin (a target human milk protein) produced in a mammalian system (transgenic goats). Although the research goal focuses...
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PHYSIOLOGICAL STATE AND PRODUCTIVITY OF CALVES FED GOAT MILK CONTAINING RECOMBINANT HUMAN LACTOFERRIThe document investigates the bioactivity of recombinant human lactoferrin (a core target protein) in young animals, providing data essential for evaluating the efficacy and application aspects...
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Polish Journal of Veterinary Sciences2018 · Journal of Veterinary Sciences Vol
Although the document focuses on HEV vaccine production using the baculovirus system, it is relevant because it specifically measures the induction of secretory IgA (sIgA) in mice. sIgA is one of the...
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PRODUCTION OF RECOMBINANT MILK-CONVERTING ENZYME IN YEAST PICHIA PASTORISJournal of Applied Biotechnology
The document details the cloning, secretion strategy (alpha-factor signal peptide), fermentation, and purification of a recombinant protein (chymosin, highly related to caseins/milk proteins) using...
2 claims
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Packaging protein drugs as bacterial inclusion bodies for therapeutic applicationsThe document details the engineering of bacterial expression platforms (E. coli) to produce high concentrations of functional recombinant proteins in the form of inclusion bodies (IBs). This...
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Patents on Life A Brief View of Human Milk Component PatentingThe document details patents regarding the production of key human milk proteins (specifically lactoferrin) using genetic engineering. It explicitly names microbial systems like 'fungus, and algae'...
2 claims
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Perspective Chapter Engineering Secretory IgA against Infectious DiseasesThe document focuses entirely on Secretory IgA (SIgA), a key target human milk protein listed in the research goal. It directly addresses the required metrics, discussing strategies for improving...
3 claims
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Perspectives on Immunoglobulins in Colostrum and Milk2011
The document reviews immunoglobulins (sIgA is a target protein) in milk, discussing their structure, stability, function, and differences between species (bovine vs. human). Although it focuses on...
3 claims
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Physicochemical Properties Determination of Recombinant Human Lysozyme and Its Effects on Intestinal2025
The document provides critical functional and physicochemical characterization (bioactivity, stability) of recombinant human lysozyme (one of the target proteins) essential for infant nutrition. This...
3 claims
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Physiological response of Pichia pastoris GS115 to methanol-induced high level production of the HepThe document investigates the physiological and proteomic response of the target expression platform (Pichia pastoris) to high-level protein expression, specifically focusing on ER stress (UPR, ERAD)...
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Physiological state as transferable operating criterion to improve recombinant protein production inThe document provides detailed bioprocess engineering data (titer, specific productivity, PGAP promoter usage, secretion signal sequence, and optimization methods like oxygen limitation) specific to...
2 claims
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Physiological response of Pichia pastoris GS115 to methanol-induced high level production of the HepThe document provides detailed physiological (proteomic, ultrastructural) analysis of high-level protein production in Pichia pastoris, a key expression platform. Findings regarding ER stress (UPR),...
3 claims
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Pichia pastoris-Expressed Dengue 2 Envelope Forms Virus-Like Particles without Pre-Membrane Protein2013
The document provides specific, detailed technical data essential for the research goal's comparative analysis of expression platforms. It focuses on Pichia pastoris (a required platform), detailing...
3 claims
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Pichia pastoris-expressed dengue 3 envelope-based virus-like particles elicit predominantly domain I2015 · Frontiers in Microbiology
Although the document focuses on expressing Dengue Envelope protein (a vaccine candidate) instead of human milk proteins, it directly addresses the performance characteristics (titer, PTM fidelity,...
2 claims
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Pichia pastoris Aft1 - a novel transcription factor, enhancing recombinant protein secretionThe document investigates strain engineering in Pichia pastoris (a target expression platform) by overexpressing the Aft1 transcription factor to enhance recombinant protein secretion. This directly...
2 claims
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Pichia pastoris an Ideal Host for the Production of Recombinant Influenza Vaccines2023 · Journal of Veterinary Medicine
The document, while focused on influenza vaccines, is a review detailing the characteristics and optimization strategies of Pichia pastoris (a key platform in the research goal). It specifically...
3 claims
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Pichia pastoris and the Recombinant Human Heterodimeric Amino Acid Transporter 4F2hc-LAT1 From Clone2021
The document provides detailed, transferable methodological protocols (clone selection strategy, optimization, expression conditions, yield metrics) for expressing a complex human N-glycoprotein in...
3 claims
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Plant Production of Vaccine Against HPV A New PerspectivesThe document discusses different expression systems (Baculovirus, Saccharomyces, Plants) for vaccine production. It explicitly mentions Saccharomyces cerevisiae as the platform used for commercial...
2 claims
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Pichia pastoris versus Saccharomyces cerevisiae a case study on the recombinant production of human2017
The document directly compares two target expression platforms (Pichia pastoris and Saccharomyces cerevisiae) using the key metrics specified in the research goal: titer/secretion (285 mg/L vs 64...
3 claims
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Plant-based solutions for veterinary immunotherapeutics and prophylacticsThe document focuses on plant expression platforms and veterinary products, which are outside the core scope (microbial systems producing HMPs). However, it directly addresses critical comparison...
3 claims
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Plant flavones enhance antimicrobial activity of respiratory epithelial cell secretions against Pseu2017 · PLOS ONE
The document investigates the enhanced antimicrobial activity of recombinant human lysozyme, which is one of the key bioactive human milk proteins listed in the research goal. While the document does...
2 claims
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Plasmid-based and genome-based expression of recombinant T1 lipase in sucrose-utilizing E. coli straMol. Biol. Biotechnol.
Although the document studies T1 lipase (not a human milk protein) and does not address glycosylation, it provides specific, transferable strain engineering methods (genome integration vs. plasmid...
3 claims
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Plasmids for Optimizing Expression of Recombinant Proteins in i E. coli iThe document details methods and constraints for optimizing the bacterial expression platform (E. coli), which is one of the key platforms listed in the research goal. It specifically addresses...
1 claim
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Plastid transformation biotechnology increasing efficiency and expanding applicationThe document investigates plastid transformation in plants as a high-level expression platform for therapeutic proteins, explicitly noting its potential to overcome manufacturing hurdles...
2 claims
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Platforms for Production of Protein-Based Vaccines From Classical to Next-Generation Strategies2021
The document reviews and compares the performance and engineering strategies of key expression platforms (E. coli, S. cerevisiae, etc.) which are the central focus of the research goal (bacteria,...
3 claims
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Pleurotus ostreatus hydrophobins surface active proteinsThe document provides detailed methodological and quantitative data on protein production, secretion, and heterologous expression/purification (including titer quantification, e.g., 60 mg/L in...
2 claims
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Porcine and Bovine Forms of Lactoferrin Inhibit Growth of Porcine Enterotoxigenic Escherichia coli aThe research goal requires assessing the bioactivity and PTM fidelity of recombinant lactoferrin produced in different expression systems. This document provides detailed methodology and functional...
3 claims
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Possible response of molecular biotechnology to call SARS-CoV-2The document discusses the application of recombinant lactoferrin (one of the specific bioactive human milk proteins listed in the Research Goal) as a preparation derived using modern molecular...
3 claims
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Posttranslationale Modifikationen rekombinanter Proteine in Baculovirus-infizierten Insektenzellen1999 · Doz. Dr.
Although the expression platform (Baculovirus/Insect cells) and specific protein (ß-trace protein) are not listed in the research goal (which focuses on yeast/fungi/bacteria expressing human milk...
3 claims
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Potential Applications of Endo-β-N-Acetylglucosaminidases From Bifidobacterium longum Subspecies inf2021 · Frontiers in Nutrition
The document reviews the importance of N-glycans (a key PTM) on milk glycoproteins (target proteins like lactoferrin and caseins) for infant formula bioactivity. This information is crucial for the...
3 claims
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Potential Benefits of Bovine Colostrum in Pediatric Nutrition and Health2021
The document, while focused on bovine colostrum (BC) nutrition, provides extensive clinical and functional context regarding bioactive human milk proteins (Igs, caseins, lactoferrin) in pediatric...
2 claims
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Potential Use of Biological Proteins for Liver Failure Therapy2015
The document, although focused on liver therapy, is relevant because it discusses one of the target human milk proteins, **Lactoferrin (Lf)** (Table 1). Furthermore, it explicitly mentions the use of...
3 claims
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Potential of Lactoferrin in the Treatment of Lung Diseases2023
The document is a review focusing on the biological functions and therapeutic bioactivity of Lactoferrin (LF), a key target protein in the research goal. Understanding the specific bioactivity...
1 claim
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Preparation and Characterization of Hydroxylated Recombinant Collagen by Incorporating Proline and H2024
The document details specific strain engineering techniques (CRISPR knockout to create an auxotroph, substrate incorporation) and outcomes (titer of 1.186 g/L, PTM fidelity control, bioactivity...
3 claims
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Probiotic Yeasts A Developing Reality2024
The document reviews key expression platforms (Saccharomyces, Pichia) relevant to the research goal. It specifically discusses the use of genetic engineering to create next-generation yeast...
3 claims
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Preparation of Recombinant Membrane Proteins from i Pichia pastoris i for Molecular InvestigationsThe document provides detailed protocols and methodological data for recombinant protein production in Pichia pastoris (one of the target expression platforms). Although it focuses on membrane...
3 claims
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Preventing Sepsis in Preterm Infants with Bovine Lactoferrin A Randomized Trial Exploring Immune and2025
The document focuses on the bioactivity and clinical effectiveness of lactoferrin in infant nutrition, which is a core component of the research goal (assessing PTM fidelity and bioactivity). It...
1 claim
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Process development for the continuous production of heterologous proteins by the industrial yeast,2018
The document investigates the expression of one of the target human milk proteins, human lysozyme (HuLy), in one of the target expression platforms, Komagataella phaffii (Pichia pastoris). It...
3 claims
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Process engineering of Pichia pastoris cultivation for the production of a phytase with GAP promoterThe document provides detailed process engineering, kinetics, and promoter data (GAP) for the Pichia pastoris expression platform. Although it produces phytase, the methods for optimizing high cell...
3 claims
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Processing of predicted substrates of fungal Kex2 proteinases from Candida albicans, C. glabrata, SaThe document investigates Kex2 proteinases, which are essential for site-specific proteolysis and maturation (PTM fidelity) of secreted proteins in key expression platforms like Pichia pastoris and...
3 claims
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Processing of recombinant human IgGs in agroinfiltrated leaves of Nicotiana benthamianaThe document investigates core challenges highly relevant to the research goal's metrics (titer and secretion), specifically degradation of recombinant proteins (IgGs, which are related to sIgA, a...
3 claims
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Production and Characterization of Recombinant Human Lactoferrin in Transgenic Javanica Rice2005
The document investigates the production of a target human milk protein (lactoferrin) and provides crucial comparative data relevant to the research goal's metrics: titer (15% of TSP), strain...
3 claims
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Production and assessment of novel unspecific peroxygenases through modular yeast secretion systemsJun. Prof. Dr.
The dissertation focuses on optimizing heterologous protein secretion in key expression platforms identified in the research goal (yeast, specifically Pichia pastoris), detailing essential elements...
3 claims
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Production and secretion of recombinant proteins using Bacillus megaterium2007 · Prof. Dr.
The document investigates the 'Production and secretion of recombinant proteins' using 'Bacillus megaterium,' which falls under the 'bacteria' expression platform category relevant to the research...
3 claims
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Production of Biologically Active Cecropin A Peptide in Rice Seed Oil Bodies2016 · PLOS ONE
Although the document uses a plant system (rice) to produce Cecropin A (not a target human milk protein), it details advanced protein engineering strategies (using strong promoters, oleosin fusion...
1 claim
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Production of Chimeric Acidic α-Amylase by the Recombinant Pichia pastoris and Its Applications2017 · Frontiers in Microbiology
The document provides detailed strain construction data and engineering outcomes (AOX promoter, multi-copy generation, PTVA method) specifically aimed at enhancing titer and secretion in one of the...
1 claim
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Production of Human Pancreatitis-Associated Protein (hPAP) in Komagataella phaffii (Pichia pastoris)The document investigates the expression of a recombinant human protein in Komagataella phaffii (Pichia pastoris), which is a target expression platform. It provides transferable data on key metrics...
3 claims
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Production of Plant-Based Meat Flavor Compound – Leghemo-Globin (LegH) in Recombinant <em>KomaThe document investigates high-density fed-batch fermentation protocols and yield optimization strategies (titer, secretion metrics) for Komagataella phaffii (Pichia), which is a core expression...
3 claims
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Production of Recombinant Human Trypsinogen in Escherichia Coli and Pichia PastorisThe document provides a direct, side-by-side comparison of two key expression platforms mentioned in the research goal (E. coli/bacteria and P. pastoris/Pichia) using essential metrics: titer/yield,...
2 claims
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Production of Recombinant Humanized Anti-HBsAg Fab Fragment from Pichia pastoris by Fermentation2004 · Journal of Biochemistry and Molecular Biology
The document provides detailed, practical, high-titer secretory expression data (titer 420-458 mg/L, detailed fermentation protocol, strain construction/promoter usage) for a complex recombinant...
3 claims
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Production of Recombinant Proteins by Agrobacterium-Mediated Transient ExpressionAlthough the primary expression platform discussed is plant-based (Agrobacterium/N. benthamiana) and not yeast/fungi/bacteria, the document provides highly detailed methods on eukaryotic expression...
1 claim
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Production of Therapeutic Proteins in PlantsThe document discusses the production of therapeutic proteins in plants, an alternative expression platform. Crucially, it highlights research on producing 'anti-infective and nutritional human milk...
3 claims
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Production of Transgenic-Cloned Pigs Expressing Large Quantities of Recombinant Human Lysozyme in Mi2015 · PLOS ONE
The document investigates one of the target proteins (human lysozyme) and measures critical metrics (titer, bioactivity) related to infant nutrition. Although the expression system is transgenic pigs...
3 claims
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Production of Value-Added Products as Food Ingredients via Microbial Fermentation2023
The document is a review of microbial fermentation platforms (Pichia, Saccharomyces, bacteria, fungi) for producing food ingredients. Table 1 specifically lists the expression of lysozyme (a target...
2 claims
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Production of a 135-residue long N-truncated human keratinocyte growth factor 1 in Escherichia coliThe document provides detailed, quantitative engineering and titer data (0.4 g/L yield, solubility strategy using TRX, truncation analysis) for producing a recombinant human protein in the bacterial...
2 claims
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Production of a 135-residue long N-truncated human keratinocyte growth factor 1 in Escherichia coli2023
The document investigates optimization, yield (titer), structural stability (truncation), and purification strategies for producing a recombinant protein in E. coli (a required expression platform)....
3 claims
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Production of a Novel Superoxide Dismutase by Escherichia coli and Pichia pastoris and Analysis of t2022 · Frontiers in Nutrition
The document directly compares the heterologous expression of a protein (Superoxide Dismutase) in two platforms relevant to the research goal: E. coli (bacteria) and Pichia pastoris (yeast). It...
3 claims
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Production of a Recombinant Non-Hydroxylated Gelatin Mimetic in Pichia pastoris for Biomedical Appli2019
The document investigates expression, secretion efficiency (titer of 3.4 g/L), strain construction (AOX1 promoter), and PTM fidelity (specifically ruling out glycosylation) of a recombinant...
3 claims
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Production of a Recombinant Non-Hydroxylated Gelatin Mimetic in Pichia pastoris for Biomedical Appli2019
Although the target protein (Gelatin Mimetic) is not a human milk protein, the document provides specific, transferable performance data on one of the required expression platforms (Pichia pastoris)....
3 claims
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Production of a Recombinant Non-Hydroxylated Gelatin Mimetic in Pichia pastoris for Biomedical Appli2019
The document provides detailed technical data on the performance of the Pichia pastoris expression platform (one of the required platforms) regarding key research parameters. Specifically, it reports...
2 claims
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Production of a Recombinant Single-Domain Antibody for Gluten Detection in Foods Using the Pichia pa2020
The document details the production methodology, vector construction (promoters/restriction sites), titer (330 mg/L), and discussion of PTMs (glycosylation, disulfide bonds) using Pichia pastoris....
2 claims
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Production of a recombinant peroxidase in different glyco-engineered Pichia pastoris strains a morph2018
The study directly investigates the impact of glyco-engineering (specifically Man5 structures via OCH1 knockout) on key metrics including titer/productivity, morphology, and protein quality...
3 claims
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Production of a recombinant protein using Pichia pastoris grown in kraft evaporator condensateThe document investigates the performance (titer, yield, growth kinetics) and optimization of the Pichia pastoris expression platform, which is one of the key systems under comparison in the research...
3 claims
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Production of biliverdin by biotransformation of exogenous heme using recombinant Pichia pastoris ce2024
The document provides highly specific, quantitative data (titer, strain construction, optimization of methanol induction, pH, sorbitol concentration) on the performance and engineering of Pichia...
2 claims
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Production of bio-xylitol from&nbsp;D-xylose by an engineered&nbsp;Pichia pastoris&nbsp;The document is a detailed case study on maximizing heterologous protein expression (xylose reductase) in one of the key expression platforms (Pichia pastoris) listed in the research goal. It...
3 claims
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Production of di-D-Fructofuranose 1,2´ 2,3´ Dianhydride (DFAIII) using Recombinant Inulase IIProf. Dr.
Although the target protein (Inulase II) is incorrect, this PhD thesis provides detailed, high-level process engineering data (strain construction, fed-batch strategies, exponential feeding, HCDF...
3 claims
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Production of fluorescent and cytotoxic K28 killer toxin variants through high cell density fermenta2017
The document provides a detailed case study on optimizing heterologous expression, secretion, high yield, and bioactivity assessment of a complex protein using Pichia pastoris, one of the key...
2 claims
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Production of human fucosyltransferases in Trichoderma reeseiThe document investigates detailed strain engineering and expression strategies (promoters, signal peptides, secretion optimization, culture conditions) in a specific filamentous fungal host...
3 claims
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Production of recombinant bacteriocin divercin V41 by high cell density Escherichia coli batch and f2007
The document investigates high-density culture optimization (batch/fed-batch, IPTG induction) in Escherichia coli, which is one of the required expression platforms (bacteria). The findings regarding...
1 claim
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Production of recombinant human lactoferrin from transgenic plants2004
The document focuses on one core target protein (lactoferrin) and details crucial comparative metrics required by the goal, such as titer, PTM fidelity (glycosylation analysis), and bioactivity....
3 claims
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Production of recombinant proteins in E. coli by the heat inducible expression system based on the pThe document provides detailed technical information (promoter function, stress response, induction strategies) about an important expression system used in one of the target platforms (bacteria/E....
3 claims
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Production of recombinant proteins in milk of transgenic and non-transgenic goats1998 · Braz. Arch. Biol. Technol.
The document, while focusing on transgenic goats, directly addresses the key metrics and challenges of the research goal. It explicitly contrasts the PTM fidelity and glycosylation capabilities of...
3 claims
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Production of secretory cutinase by recombinant Saccharomyces cerevisiae protoplasts2016
The document investigates a critical strain engineering strategy (protoplast use/immobilization) in a required expression platform (Saccharomyces cerevisiae) to overcome the bottleneck of...
3 claims
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Production of soluble eukaryotic recombinant proteins in E. coli is favoured in early log-phase cultThe document optimizes key production metrics (titer and solubility/functionality) for expressing eukaryotic proteins in E. coli, which is one of the specific 'bacteria' expression platforms listed...
2 claims
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Production of transglutaminase in glutathione-producing recombinant Saccharomyces cerevisiae2021
The document investigates strain engineering in a key target expression platform (*Saccharomyces cerevisiae*) to improve the production and activity of a heterologous enzyme. Crucially, the activity...
3 claims
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Production of β‐galactosidase from recombinant i Saccharomyces cerevisiae i grown on lactoseJournal of Chemical Technology and Biotechnology
The document investigates the optimization of extracellular protein production (titer and secretion) in a target expression platform (*Saccharomyces cerevisiae*) using specific promoter systems...
2 claims
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Production systems for recombinant antibodiesFrontiers in Bioscience
The document provides a detailed comparative review of the exact expression platforms (bacteria, yeast, filamentous fungi) listed in the research goal, focusing explicitly on the key comparison...
3 claims
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Produktion eines Derivats des antimikrobiellen Peptids NK-Lysin in E. coli und P. pastorisProf. Dr.
The document provides a direct comparison of expression strategies, yields (titer), and engineering challenges between two platforms specified in the research goal: the bacterium E. coli and the...
3 claims
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Produktion rekombinanter Antikörperfragmente in Bacillus megaterium2007
The document investigates the production and secretion of recombinant proteins using Bacillus megaterium, which falls under the 'bacteria' expression platforms specified in the research goal. It...
3 claims
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Progress in Ameliorating Beneficial Characteristics of Microbial Cellulases by Genetic Engineering A2020 · Frontiers in Microbiology
Although the document focuses on microbial cellulases (not human milk proteins), it is a detailed review of genetic engineering strategies (promoters, signal peptides/leader peptides, chaperones,...
3 claims
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Properties, production, and applications of camelid single-domain antibody fragments2007
The document focuses heavily on the production of heterologous proteins (VHH antibodies) in specific expression platforms (microorganisms, with a focus on yeasts, which includes Pichia and...
3 claims
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Prophylactic and Therapeutic Role of Human Breast Milk Proteins and Bioactive Peptides against NeonaThe document provides crucial foundational context for the research goal by focusing intensely on the bioactivity and clinical relevance of human milk antimicrobial proteins and peptides (which...
2 claims
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Prospects of biosimilar recombinant human growth hormone manufacture in the Republic of MoldovaThe document provides detailed strain construction and platform comparison data highly relevant to the goal. It describes the use of Pichia pastoris (a target platform), selection based on PTM...
3 claims
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Protection of Human Colon Cells from Shiga Toxin by Plant-based Recombinant Secretory IgAThe document directly studies the heterologous production of one key target protein (secretory IgA, sIgA), focusing on multi-chain assembly, post-translational modification fidelity (plant-specific...
3 claims
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Protein Bodies in Nature and Biotechnology2012
The document discusses protein bodies (IBs/PBs) in bacteria and mammalian systems, which directly relates to the key research metrics of titer and secretion efficiency for heterologous proteins. It...
3 claims
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Protein Glycoengineering An Approach for Improving Protein Properties2020 · Frontiers in Chemistry
The document is a review focusing entirely on Protein Glycoengineering (PTM fidelity), which is a crucial comparative factor (glycosylation fidelity) specified in the research goal for different...
3 claims
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Protein expression in Pichia pastoris recent achievements and perspectives for heterologous protein2014
The document is a detailed review focused on protein expression in Pichia pastoris, which is one of the key expression platforms specified in the Research Goal. It provides crucial data and methods...
3 claims
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Protein expression in Komagataella phaffii (formerly Pichia pastoris) v1The document provides a detailed, working protocol for recombinant protein expression in Komagataella phaffii (formerly Pichia pastoris), which is explicitly listed as one of the key expression...
2 claims
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Protein folding and conformational stress in microbial cells producing recombinant proteins a host cThis document is a comparative review of recombinant protein production across the key expression platforms listed in the research goal (yeasts, filamentous fungi, bacteria). It specifically...
3 claims
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Protein fusion tags for efficient expression and purification of recombinant proteins in the peripla2016
The document provides detailed strain engineering data, focusing on signal peptides (translocation), fusion tags, solubility, secretion (periplasmic space), and proper folding (disulfide bond...
3 claims
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Protein histidine phosphatase structural aspects and identification of the ATP-citrate lyase as a suThe document appears to be a technical description or patent related to recombinant protein expression, evidenced by terms related to vector components ('promoter,' 'signal sequence,' 'vector') and...
3 claims
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Protein production dynamics and physiological adaptation of recombinant Komagataella phaffii at nearThe document investigates protein production dynamics and physiological adaptation in Komagataella phaffii (Pichia pastoris), which is one of the key expression platforms specified in the research...
3 claims
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Protein production dynamics and physiological adaptation of recombinant Komagataella phaffii at near2024
The document investigates protein production dynamics, specific productivity (qP), and limitations in the secretory pathway for a key target expression platform, Komagataella phaffii (Pichia...
2 claims
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Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretiThe document provides detailed strain engineering data and outcomes (increased titer/secretion) for Pichia pastoris, which is one of the expression platforms targeted by the research goal. The...
3 claims
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Proteomic characterization of yeast secretion mutantsThe document focuses on characterizing engineered secretion mutants in Saccharomyces cerevisiae (a target expression platform) using proteomic approaches. It directly addresses key metrics relevant...
3 claims
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Proteomics and biotechnologies new methods for glycoproteome analysisThe document focuses on developing advanced mass spectrometry and proteomics methods for characterizing protein glycosylation and glycoforms (PTM fidelity), which is a crucial parameter in the...
3 claims
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Purification Method Optimization of Recombinant Platelet-Derived Growth Factor rhPDGF-BB Expressed iThe document, while focusing on rhPDGF-BB, uses and details one of the core expression platforms mentioned in the research goal (Pichia pastoris). It provides critical technical information regarding...
2 claims
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Purification and Characterization of Recombinant Human Lysozyme from Eggs of Transgenic Chickens2015 · PLOS ONE
The document focuses on one of the key target proteins (human lysozyme) and addresses the required metrics (titer/purification efficiency, bioactivity, PTM fidelity/glycosylation). While the...
3 claims
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Purification and biological activity of recombinant human bone morphogenetic protein-2 produced by EThe document, while focusing on BMP-2, provides detailed technical data on one of the target expression platforms (*E. coli*/bacteria). It specifically addresses critical comparative criteria:...
3 claims
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Purification and characterization of recombinant human bile salt-stimulated lipase expressed in milk2017 · PLOS ONE
Although the expression platform is transgenic cattle, which is outside the stated scope (microbial systems), the document provides critical benchmarking data for the production of a bioactive human...
3 claims
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Purification and characterization of the first recombinant bird pancreatic lipase expressed in PichiThe document provides detailed strain construction data (GAP promoter, secretion results, multi-copy integration estimation via Q-PCR), titer, and secretion results for heterologous protein...
3 claims
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Purification of Modified Therapeutic Proteins Available on the Market An Analysis of Chromatography-The research goal requires assessing PTM fidelity (glycosylation) and regulatory viability for various expression systems. While the document focuses on therapeutic proteins rather than HMPs, it...
2 claims
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Purification of heterologous membrane proteins Cytochrome P450 CYP81A9 and Homogentisate SolanesyltrProf. Dipl.
The document investigates the heterologous production and purification optimization (solubilisation, chromatography) of recombinant proteins in Pichia pastoris, one of the key expression platforms...
2 claims
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Putting the Spotlight Back on Plant Suspension Cultures2016 · Frontiers in Plant Science
The document is a review that explicitly compares and contrasts the characteristics (protein folding, PTM fidelity, cost, complexity) of several expression platforms central to the research goal,...
3 claims
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Quantification of extracellular UDP-galactoseThe document describes a highly sensitive analytical assay for quantifying UDP-galactose (UDP-Gal), which is the essential donor substrate for galactosylation. Optimizing UDP-Gal metabolism and...
2 claims
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Quantifying the Effects of Frequency and Amplitude of Periodic Oxygen-Related Stress on Recombinant2013
The document investigates optimization strategies to improve titer and specific productivity in Pichia pastoris, which is one of the target expression platforms. The findings regarding the effect of...
3 claims
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Quantitative Analysis of Antibody Survival across the Infant Digestive Tract Using Mass Spectrometry2020
The document provides highly transferable, validated methodology (LC-MS/MS PRM with PEG normalization) for quantitatively determining the intact survival rate and degradation profile of recombinant...
3 claims
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Quantitative Metabolomics and Instationary 13C-Metabolic Flux Analysis Reveals Impact of Recombinant2014
The document investigates the fundamental metabolic burden (energy, carbon flux, trehalose stress response) imposed by secreted recombinant protein production in Pichia pastoris. Pichia is a key...
3 claims
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Quantitative characterization of a recombinant Pichia pastoris Mut+ strain secreting avidin using tr2007
The document is a PhD thesis providing detailed quantitative characterization of the Pichia pastoris expression platform, focusing on titer, secretion, AOX promoter regulation, process optimization...
3 claims
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Quantitative comparison of dynamic physiological feeding profiles for recombinant protein production2013
The document focuses on optimizing fed-batch cultivation parameters (titer/productivity, specific substrate uptake rate, product quality) for Pichia pastoris, which is one of the key expression...
2 claims
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Quantitative metabolomics analysis of amino acid metabolism in recombinant Pichia pastoris under difThe document provides detailed physiological, metabolomic, and specific productivity (titer/secretion) data for the Pichia pastoris expression platform under various conditions (oxygen availability)....
3 claims
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Quercetin and a plant substance, identified using a multi-copy number of DNA topoisomerase I in recoThe document uses Pichia pastoris, one of the required expression platforms, and provides detailed, transferable strain construction data (multi-copy gene integration strategy using pPIC3.5K and...
3 claims
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Quercetin and a plant substance, identified using a multi-copy number of DNA topoisomerase I in recoThe document provides detailed, transferable strain engineering methods and optimization strategies (multi-copy number integration using pPIC3.5K vector, Geneticin selection, focus on increasing...
2 claims
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Rapid and Scalable Plant-based Production of a Cholera Toxin B Subunit Variant to Aid in Mass Vaccin2013
Although the document focuses on CTB production in plants, it directly addresses critical parameters of the research goal, including PTM fidelity (N-glycosylation), optimization of secretory...
2 claims
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Rational design of cyclic peptide inhibitors of matriptaseThe document is a PhD thesis that dedicates a chapter (Chapter 3) to the recombinant expression of a heterologous human protein (Matriptase) using two key expression platforms mentioned in the...
3 claims
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Rational development of bioprocess engineering strategies for recombinant protein production in PichThe document is a review focusing on bioprocess engineering and strain optimization (promoters, yields, productivity) for recombinant protein production (RPP) using Pichia pastoris. Pichia is one of...
3 claims
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Rationale und modellbasierte Charakterisierung von heterologer Genexpression in biologischen SystemeProf. Dr.
This PhD thesis details rational and model-based characterization (DOE, automation) of heterologous gene expression, focusing on optimizing strain construction (vectors, A. tumefaciens) and improving...
3 claims
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Rationalizing PINK1 transmembrane mutations in Parkinson's diseaseThe document discusses the heterologous expression, cloning, and purification protocols for recombinant human proteins (PINK1 and PARL) using two of the target expression platforms: Escherichia coli...
3 claims
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Ratlarda Lipopolisakkarit İndüklü Deneysel Endotoksemi Modelinde Adenozin Deaminaz, Nitrik Oksit ve2020
The research goal requires assessing the bioactivity and PTM fidelity of human milk proteins, specifically including lactoferrin. This document provides experimental data detailing the protective and...
2 claims
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Raw starch conversion by Saccharomyces cerevisiae expressing Aspergillus tubingensis amylasesThe document investigates heterologous protein expression and secretion in Saccharomyces cerevisiae, which is a key platform mentioned in the research goal. Crucially, it specifically addresses the...
3 claims
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Recent Advances in Heterologous Protein Expression and Natural Product Synthesis by Aspergillus2025
The document reviews the Aspergillus expression platform (a filamentous fungus, one of the platforms listed in the goal). It specifically discusses the heterologous expression of human lysozyme and...
3 claims
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Recent Developments in Bioprocessing of Recombinant Proteins Expression Hosts and Process Developmen2019 · Frontiers in Bioengineering
The document is a comprehensive review comparing different expression host systems (bacteria, Pichia, Saccharomyces) regarding PTMs (glycosylation) and bioprocess development for maximizing...
3 claims
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Recent advances in lactoferrin research and development during the past two years (2007–2009) in lie2009 · Co. Ltd.
The document, a preface to a special issue on Lactoferrin, explicitly discusses recombinant expression platforms and outcomes for this target human milk protein. It mentions heterologous expression...
3 claims
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Recent advances in recombinant production of soluble proteins in E. coli2025
The document is a review focused on optimizing recombinant protein production in E. coli (a key bacterial platform listed in the research goal). It specifically addresses critical metrics mentioned...
2 claims
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Reciprocal Interactions between Lactoferrin and Bacterial Endotoxins and Their Role in the Regulatio2010
The research goal requires assessing the 'bioactivity' and 'PTM fidelity' of recombinant Lactoferrin (Lf). This document is a review providing detailed mechanisms and structural requirements...
1 claim
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Recognition of Microbial Glycans by Human IntelectinThe research goal requires evaluating PTM fidelity (glycosylation) and bioactivity/regulatory viability of human milk proteins expressed in microbial/fungal hosts (Pichia, Saccharomyces, bacteria)....
3 claims
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Recombinant .ALPHA.-Glucosidase from Aspergillus niger. Overexpression by Emericella nidulans, PurifThe document, while focusing on alpha-glucosidase, provides highly relevant methodological and comparison data for the research goal. It specifically uses a filamentous fungal expression platform...
3 claims
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Recombinant AfusinC, an anionic fungal CSαβ defensin from Aspergillus fumigatus, exhibits antimicrob2018 · PLOS ONE
The document provides highly detailed, practical strain engineering data, expression protocols (fusion tag, TEV cleavage, refolding from inclusion bodies), and titer (0.8 mg/L) for producing a...
2 claims
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Recombinant Aspergillus β-galactosidases as a robust glycomic and biotechnological tool2013
The document provides detailed strain engineering and expression protocols for secreted recombinant proteins in Pichia pastoris (a key platform listed in the research goal). Specifically, it mentions...
3 claims
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Recombinant Escherichia coli produces tailor-made biopolyester granules for applications in fluorescThe document investigates protein production in a bacterial platform (E. coli), which is one of the platforms targeted by the research goal. Although the specific proteins (IL2, MOG) are not human...
2 claims
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Recombinant Expression and Characterization of α-Conotoxin LvIA in Escherichia coli2016
Although the target protein (conotoxin) is irrelevant, the document provides detailed methodology and performance metrics (high titer: 100–500 mg/L) for the E. coli expression platform, which is one...
3 claims
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Recombinant Expression in Pichia pastoris System of Three Potent Kv1.3 Channel Blockers Vm24, Anuroc2022
The document focuses on the recombinant expression performance (titer, yield comparison, bioactivity as a measure of PTM fidelity/folding) using the Pichia pastoris system, which is one of the key...
3 claims
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Recombinant Expression of Serratia marcescens Outer Membrane Phospholipase A (A1) in Pichia pastoris2019 · Frontiers in Microbiology
The document provides specific, empirical data on strain construction, induction protocols, and expression titer/efficiency using Pichia pastoris (one of the key platforms listed in the research...
2 claims
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Recombinant Expression of a Modified Shrimp Anti-Lipopolysaccharide Factor Gene in Pichia pastoris G2016
The document provides transferable data on one of the target expression platforms (Pichia pastoris). It details strain construction (pPIC9K vector, alpha-factor signal peptide), codon optimization...
2 claims
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Recombinant Human Intelectin Binds Bovine Lactoferrin and Its PeptidesThe research goal involves comparing expression platforms, including bacteria (E. coli), for producing bioactive human milk proteins (HMPs) like lactoferrin (LF), with an emphasis on strain...
3 claims
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Recombinant Human L-ferritin from Saccharomyces cerevisiae Molecular Characterization and Synthesis2011
The document directly compares two expression platforms named in the research goal (Saccharomyces cerevisiae and E. coli/bacteria) for producing a human protein (L-ferritin). It addresses key metrics...
3 claims
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Recombinant Kluyveromyces lactis expressing highly pathogenic porcine reproductive and respiratory s2014 · Vet. Sci.
The document investigates the use and engineering of a yeast expression platform (*Kluyveromyces lactis*) highly relevant to the research goal's focus on fungal/yeast platforms (Pichia,...
2 claims
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Recombinant Inga Laurina Trypsin Inhibitor (ILTI) Production in Komagataella Phaffii Confirms Its Po2018
The document details the heterologous expression, vector construction (pPIC9K/AOX1 promoter), and optimization strategies for recombinant protein production in Komagataella phaffii (Pichia). Pichia...
2 claims
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Recombinant Moringa Oleifera Lectin produced in Pichia Pastoris is a Potential Natural CoagulantThe document investigates the optimization and production of a recombinant protein using one of the specified expression platforms (*Pichia pastoris*). Although the specific protein (Moringa Oleifera...
2 claims
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Recombinant O-mannosylated protein production (PstS-1) from Mycobacterium tuberculosis in Pichia pas2019
The document provides highly transferable data regarding the performance, engineering, titer (~46 mg/L), secretion (alpha-factor signal), and PTM fidelity (O-mannosylation) of the Pichia pastoris...
3 claims
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Recombinant Production and Molecular Docking Studies of Casoplatelin, a Bioactive PeptideThe document describes the recombinant production and purification of a bioactive peptide (Casoplatelin), which is derived from $\kappa$-casein. Caseins are specifically listed among the target human...
2 claims
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Recombinant Production of Bovine αS1-Casein in Genome-Reduced Bacillus subtilis Strain IIG-Bs-20-5-12025
The document reports the recombinant production, titer (56.9 mg/L), yield, and engineering strategies (genome reduction) for $\alpha$S1-casein (a target human milk protein type) using Bacillus...
3 claims
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Recombinant Production of Caspian Trout (Salmo caspius) Hepcidin in Escherichia coli and Evaluation2024 · Aquat. Sci.
Although the target protein is fish hepcidin, the document focuses on one of the key expression platforms (E. coli/bacteria) mentioned in the goal. It provides detailed, hands-on methodological data...
3 claims
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Recombinant Production of Human Aquaporin-1 to an Exceptional High Membrane Density in Saccharomyces2013 · PLOS ONE
The document investigates optimization strategies (titer, folding, strain engineering, promoter systems) and PTM fidelity (glycosylation) for heterologous protein production in Saccharomyces...
3 claims
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Recombinant Production of Hydrophobin DewA in Pichia pastoris and Determination of Its FunctionsJournal of Life Sciences and Biotechnology
The document provides detailed, transferable strain engineering methods, vector design (pPICZα-A, AOX1 promoter), optimization data (titer, induction conditions), and notes on PTM capabilities...
3 claims
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Recombinant Protein Expression System in Corynebacterium glutamicum and Its Application2018 · Frontiers in Microbiology
The document reviews the use of Corynebacterium glutamicum as a recombinant protein expression host (a bacterial platform), specifically detailing genetic components, promoters, secretory signal...
3 claims
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Recombinant Pvs48 45 Antigen Expressed in E. coli Generates Antibodies that Block Malaria Transmissi2015 · PLOS ONE
Although the target protein is a malaria antigen, the document directly addresses the strengths and weaknesses of using E. coli (a bacterial platform listed in the goal) for expressing complex...
3 claims
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Recombinant ROP6 protein expressed in Saccharomyces cerevisiae INVSc1 cells induced strong immune reThe document utilizes and provides data on one of the primary target expression platforms, Saccharomyces cerevisiae, focusing on recombinant protein production, PTM capability, and suitability for...
2 claims
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Recombinant chymosin of Camelus dromedarius in Pichia pastoris expression system Purification and EnThe document focuses on producing a recombinant milk-related enzyme (chymosin, which targets casein, a target protein class) using one of the primary expression platforms specified in the research...
3 claims
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Recombinant complement factor H in complement-mediated diseasesAlthough the target protein is Complement Factor H, not a human milk protein, the document provides highly detailed and transferable information regarding the performance and engineering of a...
3 claims
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Recombinant expression, purification and characterization of human hyaluronidasesProf. Dr.
The document is a PhD thesis detailing the recombinant expression, optimization, and characterization of a human protein (Hyaluronidase) using one of the required expression platforms (bacteria/E....
3 claims
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Recombinant expression and characterization of the endochitinase Chit36-TA from Trichoderma asperellThe document investigates recombinant protein expression, titer/purification yields (123 mg/L crude extract), secretion (supernatant analysis), and PTM fidelity (specifically mentioning the...
2 claims
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Recombinant expression and biological characterization of the antimicrobial peptide fowlicidin-2 inThe document provides detailed, high-yield engineering and expression data for a key expression platform specified in the goal (Pichia pastoris). It specifically details the use of the pPICZα-A...
2 claims
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Recombinant expression and functional characterization of cannabinoid producing enzymes in komagataeProf. Dr.
The document details strain construction, bottleneck identification (post-translational optimization, PTM fidelity/folding), and comparison of key expression platforms listed in the goal...
3 claims
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Recombinant human gelatin expression and characterizationAlthough the target protein (gelatin) is not a human milk protein, the document provides highly detailed, quantifiable strain engineering data (promoter/signal peptide usage, codon optimization,...
2 claims
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Recombinant expression of Chlamydia trachomatis major outer membrane protein in E. Coli outer membraThe document, while focusing on a vaccine antigen (MOMP), utilizes and details specific, transferable strain engineering strategies (codon harmonization, promoter strength, leader sequence...
3 claims
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Recombinant expression of a new antimicrobial peptide composed of hBD-3 and hBD-4 in Escherichia colAlthough the document focuses on producing human beta-defensin (not a human milk protein), it provides highly detailed, practical strain engineering data (codon optimization, vector construction,...
3 claims
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Recombinant expression of fungal oxidases for industrial applicationThe document investigates the recombinant expression, purification, and yield comparison of complex foreign proteins (laccases) across key expression platforms relevant to the research goal...
3 claims
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Recombinant hemagglutinin displaying on yeast reshapes congenital lymphocyte subsets to prompt optim2023 · Front. Microbiol.
The document investigates recombinant protein production and display using Saccharomyces cerevisiae, one of the key expression platforms specified in the research goal. It provides transferable data...
1 claim
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Recombinant fluorescent myristoylated PreS-containing Hepatitis B virus capsid- like particlesProf.
Dr.
The document is a detailed PhD thesis, which aligns with the requested data sources (patents/PhD theses). Although the target protein (HBV capsid) and primary Post-Translational Modification...
3 claims
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Recombinant human N-acetylgalactosamine-6-sulfate sulfatase (GALNS) produced in the methylotrophic yThe document, while studying GALNS instead of human milk proteins, provides critical, transferable data directly addressing the research goal's focus areas. It compares expression platforms (Pichia...
3 claims
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Ανάπτυξη χρωματογραφικών μεθόδων καθαρισμού (HILIC) με φασματοσκοπία μαζών (MALDI-TOF & LEMS MS)The document structure (likely a detailed thesis or technical report Table of Contents) contains extensive sections on strain engineering, expression systems (frequent use of code /0, likely Pichia...
3 claims
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Recombinant human lactoferrin enhances the efficacy of triple therapy in mice infected with HelicobaThe document investigates the bioactivity (a key metric required by the goal) of recombinant human lactoferrin (rhLf), a target protein, in an application relevant to infant nutrition (combating H....
1 claim
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Recombinant neutralizing secretory IgA antibodies for preventing mucosal carriage and transmission oThe document investigates the production and bioactivity of recombinant Secretory IgA (sIgA), which is a core target protein of the research goal (human milk proteins). Although the document uses a...
3 claims
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Recombinant polypeptide production in E. coli towards a rational approach to improve the yields of fThe document provides a detailed review of strategies and engineering outcomes (promoters, chaperones, secretion mechanisms) aimed at improving titer, yield, folding, and secretion efficiency for...
3 claims
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Recombinant production and characterization of Aspergillus niger prolyl endopeptidase enzyme for glu2021 · Journal of Agriculture
The document investigates recombinant protein production in one of the specified platforms (bacteria/E. coli) and provides quantitative data on yield (titer: 280 mg/L) and methodology (inclusion body...
3 claims
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Recombinant production of bacterial toxins and their derivatives in the methylotrophic yeast PichiaThe document reviews the use of Pichia pastoris (a key platform in the goal) and provides detailed data on strain engineering, expression strategies (promoters, synthetic genes, secretion vs....
3 claims
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Recombinant production of Horseradish peroxidase in E. coli and application in activated prodrug canProf. Dipl.
The document, a PhD dissertation, provides detailed methods and outcomes for optimizing the bacterial expression platform (E. coli), which is explicitly listed in the research goal. Although the...
2 claims
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Recombinant production of human antimicrobial peptide LL- 37 and its secondary structureThe document investigates the recombinant production strategy (fusion protein, codon optimization, high yield) of a bioactive human milk component (LL-37) in one of the required platforms (E....
3 claims
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Recombinant production of human antimicrobial peptide LL- 37 and its secondary structure2024
The document investigates the recombinant production of a bioactive human milk protein (LL-37, found in breast milk) in one of the target expression platforms (E. coli/bacteria). It focuses on...
3 claims
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Recombinant production of human microsomal cytochrome P450 2D6 in the methylotrophic yeast Pichia paProf. Dr.
The document provides highly transferable methods and comparative data concerning one of the key expression platforms, Pichia pastoris. It details strain construction (AOX1 promoter), co-expression...
3 claims
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Recombinant production of hydrophobins - HFB4 and HFB7 in trichoderma reeseiAssoc. Prof. Dr.
The document investigates recombinant protein production, secretion efficiency, and strain engineering methods (promoters, tags) in a specific platform listed in the research goal: filamentous fungi...
2 claims
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Recombinant production of peroxidase variants in E. coli and yeast – a quantitative and qualitativeAssoc. Prof. Dipl.
The document performs a direct, quantitative and qualitative comparison between two expression platforms highly relevant to the goal: E. coli (bacterium) and Pichia pastoris (yeast). The comparison...
3 claims
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Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization paThe document focuses on detailed strain engineering outcomes in Pichia pastoris (a key platform listed in the research goal) to improve recombinant protein titer and efficiency by manipulating the...
3 claims
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Recombinant protein production facility for fungal biomass-degrading enzymes using the yeast Pichia2015 · Frontiers in Microbiology
The document details optimized, high-throughput recombinant protein production protocols, vector usage (pPICZαA, pGAPZαA), signal peptides (alpha-secretion factor), and expression strategies for the...
3 claims
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Recombinant protein production provoked accumulation of ATP, fructose-1,6-bisphosphate and pyruvate2021
The research goal requires comparing bacterial (E. coli) production platforms with fungal platforms. This document provides detailed, fundamental data on metabolic constraints (metabolic burden,...
3 claims
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Recombinant sterol esterase from Ophiostoma piceae an improved biocatalyst expressed in Pichia pastoThe document investigates detailed strain construction, optimization, secretion mechanisms (alpha-mating factor), and PTM fidelity (glycosylation degree and N-terminal processing issues) when...
3 claims
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Reconstituted nonribosomal biosynthesis of the peptide antibiotic valinomycin in Escherichia coli asProf. Dr.
The document provides detailed strain engineering data, expression optimization strategies (vectors, promoters, high-cell density culture), and methods for addressing post-translational modification...
2 claims
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Redesigning Nature Developing a More Potent BMP2 Molecule for Expression in a Transgenic PuroindolinThe document, while focusing on BMP2 expression in rice and E. coli, contains highly transferable methods and data regarding recombinant protein engineering. Specifically, it details codon...
3 claims
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Reduction of Cross-Reactive Carbohydrate Determinants in Plant Foodstuff Elucidation of Clinical Rel2011
The research goal emphasizes PTM fidelity, specifically glycosylation, and bioactivity for infant nutrition. This document focuses entirely on the immunological relevance and reduction of specific...
2 claims
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Reducing conditions are the key for efficient production of active ribonuclease inhibitor in Escheri2011
The document details sophisticated strain engineering and bioprocess strategies (vector optimization, signal peptides, redox control using DTT, fed-batch scale-up) to maximize the titer, secretion,...
2 claims
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Regulating the T7 RNA Polymerase Expression in E. coli BL21 (DE3) to Provide More Host Options for RThe document provides detailed strain engineering data and optimization strategies (promoter replacement, regulation of T7 RNAP expression, managing resource allocation) specifically for the...
3 claims
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Regulation of S-layer synthesis and secretion in Caulobacter crescentusThe document provides detailed strain engineering data, outcomes, and mechanistic insights (UTR stability, post-transcriptional regulation, secretion system modification) for maximizing protein...
3 claims
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Rekombinante Antikörper-Enzym-Fusionskonstrukte zur zielgerichteten Therapie kolorektaler KarzinomeProf. Dr. Dr.
The document is a detailed academic thesis (Habilitationsschrift) that focuses on optimizing the production of recombinant fusion proteins in two key expression platforms mentioned in the research...
3 claims
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Relation of conformational and dynamic differences in collagen sequences to alpha2I domain integrinAlthough the document focuses on collagen peptides rather than human milk proteins, it provides detailed methodology (strain construction, expression outcomes, purification, and bioactivity testing...
3 claims
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Retromer and sorting nexins in developmentFrontiers in Bioscience
The document details protein sorting and trafficking mechanisms (Retromer/Vps pathways) in a key expression platform specified in the goal, Saccharomyces cerevisiae (yeast). Understanding these...
1 claim
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Review, role of lactoferrin in preventing preterm delivery2022
The document focuses on the clinical function and bioactivity of Lactoferrin (LF), a target human milk protein. It explicitly uses 'recombinant human LF' (rh-LF) in several functional experiments...
2 claims
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Review on Applications of Genetic Engineering And Cloning in Farm animals2017
The research goal compares microbial/fungal expression systems for human milk proteins (HMPs). This document reviews the application of genetic engineering in farm animals for producing...
2 claims
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Review on Bioactive Peptides in Milk and Dairy Products, and Their Health BenefitsThe document reviews the bioactivity and generation of peptides derived from key target human milk proteins (lactoferrin, lysozyme, caseins). While it focuses mainly on traditional enzymatic...
2 claims
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Revisiting Escherichia coli as microbial factory for enhanced production of human serum albumin2017
The document focuses on enhancing the soluble titer and functional production of a complex, disulfide-rich protein (HSA) in Escherichia coli (a required expression platform). The methods regarding...
2 claims
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Role of Lactobacilli and Lactoferrin in the Mucosal Cervicovaginal Defense.2018 · Frontiers in Immunology
The document extensively reviews the biological function, mechanisms of action, and bioactivity (antibacterial, antiviral, immune modulation) of Lactoferrin, which is one of the key human milk...
2 claims
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Role of Milk-Derived Antibacterial Peptides in Modern Food Biotechnology Their Synthesis, Applicatio2018
The document reviews milk-derived antibacterial peptides (ABPs) originating from key target proteins (lactoferrin, lactalbumin, caseins, lysozyme) listed in the research goal. Crucially, the abstract...
2 claims
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Role of Polymeric Immunoglobulin Receptor in IgA and IgM Transcytosis2021 · Transcytosis. Int.
The research goal specifically targets secretory IgA (sIgA) as a bioactive human milk protein. This document reviews the structure (J chain involvement) and secretion pathway (pIgR transcytosis) of...
3 claims
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Role of Proteins and of Some Bioactive Peptides on the Nutritional Quality of Donkey Milk and Their2017
The document provides crucial functional and quantitative context for several target human milk proteins (lactoferrin, alpha-lactalbumin, lysozyme, caseins). It details their concentrations in donkey...
3 claims
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Studying Lactoferrin N-Glycosylation2017
The research goal specifically focuses on comparing expression platforms for bioactive human milk proteins, emphasizing PTM fidelity (glycosylation) and bioactivity for infant nutrition. This...
3 claims
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Εκφραση και απομόνωση της ανασυνδυασμένης πρωτεΐνης Cyclin A του ανθρώπου στην Escherichia coli καιAlthough the target protein (Human Cyclin A) is not a human milk protein, the document focuses on optimizing the expression and purification of a recombinant human protein in one of the key platforms...
3 claims
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Saccharomyces Cerevisiae as a biotechnological tool for ageing research studies on translation and mThe document investigates fundamental strain engineering techniques in a key expression platform (Saccharomyces cerevisiae) to significantly increase recombinant protein yield (titer). It provides...
3 claims
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SUMO-fusion, purification, and characterization of a (+)-zizaene synthase from Chrysopogon zizanioidThe document, while focusing on a plant terpene synthase, provides detailed, quantitative data on the heterologous expression (titer, solubility optimization using SUMO-fusion tags, codon...
2 claims
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Saccharomyces cerevisiae as host for the recombinant production of polyketides and nonribosomal pept2021
The document focuses on engineering Saccharomyces cerevisiae, a key expression platform in the research goal. While the primary topic is small molecule production, the review provides foundational,...
2 claims
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Saccharomyces cerevisiae morphological changes and cytokinesis arrest elicited by hypoxia during sca2018
The document investigates critical bioprocess engineering and physiological constraints (hypoxia, scale-up, cell cycle arrest, cell wall integrity, N-acetyl-glucosamine availability) in Saccharomyces...
2 claims
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Safety Aspect of Recombinant Protein Produced by i Escherichia coli i Toxin Evaluation with Strain aThe document discusses the regulatory viability and safety aspects (toxin contamination) of E. coli, which is a key bacterial expression platform covered by the research goal. It also explicitly...
3 claims
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Sandwich ELISA for quantitative detection of human collagen prolyl 4-hydroxylaseThe document, while focusing on C-P4H instead of human milk proteins, provides highly relevant methodological and strain engineering insights. It describes optimizing the expression and yield of a...
1 claim
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Scalable methanol-free production of recombinant glucuronoyl esterase in Pichia pastorisThe document investigates optimization strategies (PGAP promoter, fed-batch kinetics, high titer: up to 2780 mg/L, high secretion efficiency) in one of the required host systems (*Pichia pastoris*)....
2 claims
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Scalable methanol-free production of recombinant glucuronoyl esterase in Pichia pastoris2019
The document investigates detailed strain engineering outcomes in one of the target expression platforms (*Pichia pastoris*). It provides specific, quantifiable data on key metrics for comparison...
2 claims
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Scalable methanol-free production of recombinant glucuronoyl esterase in Pichia pastorisThe document investigates Pichia pastoris (a target expression platform) expression strategy (constitutive PGAP promoter) and provides specific quantitative data on high titer, secretion levels, and...
2 claims
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Scalable production and application of Pichia pastoris whole cell catalysts expressing human cytochr2021
The document details scalable bioprocess optimization (fermentation strategies, HTS) and strain engineering techniques (bidirectional promoters, co-expression) specifically in Pichia pastoris, which...
2 claims
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Scalable recombinant production of bioactive human neutrophil peptide-1 in Komagataella phaffii2025
The document investigates optimizing titer and secretion of a bioactive human protein (HNP-1) using one of the key expression platforms specified in the goal (*Komagataella phaffii* / Pichia). It...
3 claims
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Scale-up Production and Immunological Assessment of Recombinant SARS- CoV-2 Spike RBD in Pichia pastThe document, while focusing on SARS-CoV-2 RBD, provides highly detailed, practical, and transferable information regarding the 'Pichia pastoris' expression platform, which is central to the research...
3 claims
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Scale-up fermentation of Escherichia coli for the production of recombinant endoglucanase from Clost2021
The document provides detailed strain engineering outcomes (silent mutations, RBS optimization) and fermentation scale-up optimization (RSM, yield/titer improvement) specifically for the bacterial...
2 claims
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Screening the anti infectivity potentials of native N- and C-lobes derived from the camel lactoferriThe research goal requires data on the bioactivity, PTM fidelity, and structure-function relationship of human milk proteins, specifically lactoferrin (LF). This document details the functional...
1 claim
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Secretion and N-Linked Glycosylation Are Required for Prostatic Acid Phosphatase Catalytic and Antin2012 · PLOS ONE
The document provides specific, practical data regarding expression parameters (titer, secretion mechanism, N-linked glycosylation fidelity, stability, and bioactivity outcomes) for a human protein...
3 claims
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Secretion of Pem-CMG, a Peptide in the CHH MIH GIH Family of Penaeus monodon, in Pichia pastoris Is2002 · Journal of Biochemistry and Molecular Biology
The document investigates detailed strain construction (signal peptides and cleavage efficiency of the alpha-factor from Saccharomyces) and reports titer (150 mg/l) and secretion outcomes in Pichia...
2 claims
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Secretion of a foreign protein from budding yeasts is enhanced by cotranslational translocation andThe document investigates bottlenecks (translocation, vacuolar targeting) in the secretion pathway of two key expression platforms mentioned in the research goal (S. cerevisiae and Pichia pastoris)....
3 claims
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Secretion of a foreign protein from budding yeasts is enhanced by cotranslational translocation andThe document investigates methods to enhance the secretion efficiency and titer of foreign proteins in two key expression platforms specified in the goal (*Saccharomyces cerevisiae* and *Pichia...
3 claims
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Secretory expression of the recombinant FGF-2 protein in Pichia pastoris carrying multiple copies ofThe document investigates secretory expression in Pichia pastoris (a target platform) and provides highly relevant engineering data, including the effect of gene copy number, use of the AOX1...
3 claims
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Selenomethionine metabolism and its toxicity in yeastThe document discusses metabolic constraints (toxicity) and engineering strategies (mutants) in Saccharomyces cerevisiae, a primary expression platform listed in the research goal. Overcoming host...
2 claims
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Semi-synthesis and biological evaluations of tunicamycin lipid analogues and investigation of the tuThe document investigates Tunicamycin, a critical biochemical tool used extensively to study N-linked glycosylation. The research goal requires assessing PTM fidelity (specifically glycosylation) in...
2 claims
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Serological Evaluation of H9-RBD-Pichia, a Novel Recombinant Influenza Vaccine, in BALB c Mice2025
The document investigates the production of a recombinant protein using Pichia pastoris, which is one of the key expression platforms specified in the research goal. Although the target protein is...
3 claims
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Serum metabolomic profile in genetically modified cows carrying human α-lactalbumin geneThe document studies one of the target human milk proteins (alpha-lactalbumin) and investigates the regulatory/safety viability of its production system (metabolomic profiles of transgenic cows)....
2 claims
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Several proteins form variable RNA editing complexes in plant organellesProf. Dr.
Although the primary topic is plant RNA editing, the document contains a detailed section (3.7 and 4.3) on using the Pichia pastoris expression system (a key platform specified in the goal). This...
3 claims
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Side effects of chaperone gene co-expression in recombinant protein productionThe document addresses methods (chaperone co-expression) and engineering challenges (yield, quality, proteolysis, folding fidelity) used to optimize the production of recombinant proteins in...
2 claims
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Signal Peptide Selection for the Efficient Periplasmic and Secretive Expression of Recombinant BrazzThe document, although studying Brazzein (not an HMP), provides highly relevant and transferable strain construction data and engineering outcomes for optimizing recombinant protein production in a...
3 claims
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Signal peptide replacement resulted in recombinant homologous expression of laccase Lcc8 in Coprinop2019
The document investigates detailed strain engineering methods (signal peptide replacement, promoter usage) to optimize the secretion and yield of a recombinant protein in a filamentous fungal host...
2 claims
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Simple and efficient expression of Agaricus meleagris pyranose dehydrogenase in Pichia pastoris2011
The document investigates the performance, scaling, and engineering of one of the core expression platforms (*Pichia pastoris*) listed in the research goal. Crucially, it provides highly relevant...
3 claims
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Simple high-cell density fed-batch technique for high-level recombinant protein production with PichThe document details a highly effective, optimized high-cell-density fed-batch protocol for maximizing recombinant protein titer using one of the target expression platforms, Pichia pastoris (AOX1...
3 claims
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Simultaneous enzyme production, Levan-type FOS synthesis and sugar by-products elimination using a r2023
The document provides detailed, empirical process data regarding the performance, yield, and regulatory viability (GRAS status) of the Pichia pastoris expression platform, which is explicitly listed...
3 claims
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Single-Cell Approach to Monitor the Unfolded Protein Response During Biotechnological Processes With2019 · Frontiers in Microbiology
The document investigates a core bottleneck (Unfolded Protein Response, UPR, and secretion limitations) in one of the primary expression platforms (Pichia pastoris) listed in the research goal....
3 claims
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Single Cell Expression Systems for the Production of Recombinant Proteins for Immunodiagnosis and Im2024
This review compares the performance of key expression platforms (prokaryotes, Pichia, yeast) in achieving correct PTMs (which affect folding and immunogenicity/bioactivity), assessing cost, and...
2 claims
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Single domain antibodies from camelids in the treatment of microbial infections2024 · Frontiers in Immunology
The research goal explicitly lists sIgA (secretory IgA) as a target protein. The document discusses engineering nanobodies by fusing them to the Fc domain of IgA to create chimeric secretory IgA...
2 claims
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Site-directed mutagenesis and purification studies of recombinant glycosylated horseradish peroxidasProf. Dr.
Although the protein studied (HRP) is not a human milk protein, the document provides detailed, high-quality data on one of the target expression platforms (P. pastoris). It specifically addresses...
3 claims
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SliC is a surface-displayed lipoprotein that is required for the anti-lysozyme strategy during NeissThe document investigates the specific bioactivity (inhibition mechanism, IC50, KD) of human c-type lysozyme, which is one of the bioactive human milk proteins targeted by the research goal. While...
2 claims
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Smart sustainable bottle (SSB) system for E. coli based recombinant protein productionThe document describes a highly efficient, low-cost bioreactor system for E. coli-based recombinant protein production, focusing on optimizing yield and solubility (titer). E. coli is one of the...
2 claims
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Soluble expression, purification, and characterization of active recombinant human tissue plasminogeThe document, while studying tPA instead of HMPs, directly addresses the core comparative aspects of the research goal: using a bacterial expression platform (E. coli) to maximize titer and achieve...
3 claims
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Soluble expression of recombinant midgut zymogen (native propeptide) proteases from the Aedes aegyptThe research goal specifically investigates the bacterial expression platform (E. coli) for complex proteins. This document provides highly detailed strain engineering and methodological data (use of...
3 claims
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Some metal binding properties of recombinant human lactoferrin from the milk of transgenic goatsThe document investigates the bioactivity (metal-binding properties) of recombinant human lactoferrin (a target protein). Although the expression platform used is transgenic goats (not the microbial...
3 claims
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Special Issue “New Insights into Lactoferrin”2025 · Mol. Sci.
The document, an editorial on lactoferrin (a target protein), explicitly addresses the research goal criteria concerning expression platforms and bioactivity. It mentions a specific biotechnological...
1 claim
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Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pasto2019
The document investigates optimization strategies (specific growth rate, gene dosage, promoter regulation, fed-batch kinetics, and titer/qp) for the Pichia pastoris expression platform. Pichia is one...
2 claims
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Spore-forming bacteria as pharmaceutical factoriesThe document is a detailed PhD thesis focusing on strain construction, optimization, and production outcomes (titer/stability) using a required expression platform (Bacillus/bacteria). The methods...
3 claims
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Stable expression of HIV-1 MPER extended epitope on the surface of the recombinant probiotic bacteri2024
Although the target protein is HIV MPER (not a human milk protein), the document provides direct and quantitative data on key comparison metrics for a bacterial expression platform (E. coli Nissle...
3 claims
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Steric accessibility of the N-terminus improves the titer and quality of recombinant proteins secret2022
The document directly addresses critical engineering challenges (titer, secretion, protein quality, signal peptide optimization, N-terminal processing) within one of the key expression platforms...
3 claims
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Strategies for Optimizing the Production of Proteins and Peptides with Multiple Disulfide Bonds2020
The document directly compares key expression platforms (E. coli, Pichia pastoris, Saccharomyces cerevisiae) regarding crucial factors for producing complex recombinant proteins, specifically yield,...
3 claims
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Strategies for successful recombinant expression of disulfide bond-dependent proteins in EscherichiaThe research goal investigates different expression platforms, including bacteria, for producing complex, secreted proteins (HMPs). Human milk proteins like lactoferrin and lysozyme are...
2 claims
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Strategies to Enhance Periplasmic Recombinant Protein Production Yields in Escherichia coliThe document focuses on enhancing titer, secretion mechanisms (periplasmic targeting, signal peptides), and folding optimization (disulfide bond formation) in *E. coli*, which is a key bacterial...
3 claims
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Structural, Functional and Phylogenetic Analysis of Sperm Lysozyme-Like ProteinsPLOS ONE
Although the document focuses on native sperm lysozyme-like proteins (LYZL) in buffalo, these proteins belong to the c-type lysozyme family, which also includes the target proteins lysozyme and...
2 claims
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Structural Analysis of Breast-Milk α sub S1 sub -Casein An α-Helical Conformation Is Required for TL2024 · Stimulation. Int.
The document focuses on the structural requirements (alpha-helical conformation) and the role of post-translational modification (phosphorylation) in regulating the bioactivity (TLR4 stimulation) of...
3 claims
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Structural Characterization of the Interaction of Human Lactoferrin with Calmodulin2012 · PLOS ONE
The document focuses on characterizing the structural basis of bioactivity for one of the target proteins (human lactoferrin) by defining its interaction with calmodulin. While it does not discuss...
1 claim
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Structural and functional investigation of Glycocin-GlycosyltransferasesProf. Dr.
The document investigates Glycocin-Glycosyltransferases (GTs), focusing on their structure, function, specificity, kinetics, and methods for heterologous expression and in vivo production of...
3 claims
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Structural and functional analysis of CD81-Claudin-1, a hepatitis C virus receptor complexAlthough the target protein (CD81-Claudin-1) is not a human milk protein, the document is highly relevant because it provides detailed, practical methodological data regarding two key expression...
3 claims
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Structural and functional features of self-assembling protein nanoparticles produced in endotoxin-fr2016
The document investigates engineered bacterial expression platforms (endotoxin-free E. coli) and measures the resulting impact on recombinant protein architecture, quality control, and in vivo...
3 claims
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Structural biology by NMR spectroscopyChapter 5 focuses on the expression, purification, and refolding challenges of hen egg-white lysozyme (HEWL) as a recombinant protein in E. coli. Lysozyme is a specific target protein mentioned in...
2 claims
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Structural biology workflow for the expression and characterization of functional human sodium gluco2019
The document investigates the heterologous expression workflow of a human protein in a key expression platform specified in the research goal (Pichia pastoris). Although the target protein is hSGLT1...
2 claims
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Structure-function correlates in human apolipoprotein A-1The document investigates expression optimization, secretion mechanisms (propeptide/signal peptide role), and functional characterization of a recombinant protein (Apo A-I). Although the protein and...
3 claims
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Structure-function studies of processing alpha-glucosidase-iThe document, a PhD thesis, focuses entirely on the structure-function and heterologous expression of Processing alpha-Glucosidase-I (Glu-I), an enzyme critical for N-glycosylation fidelity (a key...
3 claims
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Structure and domain dynamics of human lactoferrin in solution and the influence of Fe(III)-ion ligaThe document provides detailed structural and dynamic analysis of human lactoferrin's crucial functional mechanism (Fe(III) binding/domain closure). This functional reference data is essential for...
3 claims
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Structure and function studies of the filarial nematode excretory-secretory glycoprotein ES-622017
The document, while focusing on a nematode glycoprotein (ES-62) rather than a human milk protein, addresses core aspects of the research goal. Specifically, it describes the establishment of two...
3 claims
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Structure and mechanics of protein stabilized interfacesDipl. Natw.
The doctoral thesis focuses on the structure, mechanics, and interfacial rheology of protein-stabilized interfaces (emulsification/foaming), which is highly relevant to the 'bioactivity' and...
3 claims
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Struktur und Funktion des Adhäsionsrezeptors L-SelektinProf. Dr.
Although the target protein is L-Selectin (not an HMP), the document directly addresses core aspects of the research goal: it uses one of the target expression platforms (Pichia pastoris) and...
3 claims
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Studies of Cellulose and Starch Utilization and the Regulatory Mechanisms of Related Enzymes in Fung2020
The document reviews the utilization and regulation of enzymes (cellulases, amylases) in filamentous fungi. While the specific enzymes and goal (biomass degradation) differ from the research goal...
3 claims
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Studies of the structure and function of recombinant human hephaestin2011
The document provides detailed strain construction, expression, and functional analysis methodologies for expressing a complex human glycoprotein (Hephaestin) using Pichia pastoris, one of the key...
3 claims
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Studies on Recombinant Lipase Production by E. Coli Effect of Media And Bacterial Expression SystemThe document, while focusing on a non-target protein (lipase), provides highly specific, practical, and detailed experimental data on optimizing recombinant protein production (titer, host/vector...
3 claims
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Studies on the Binding Specificity of IntelectinThe document, a PhD thesis, provides direct comparative data on expression platforms, specifically noting the unsuccessful attempt to express the recombinant protein (Intelectin) in Pichia pastoris...
3 claims
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Studies on the role of protein secretion systems in Pseudomonas fluorescens biofilm formation, and dProf. Dr.
The document focuses on two key aspects directly relevant to optimizing bacterial expression platforms (one of the target platforms in the research goal): detailed analysis of Type 1 Secretion...
3 claims
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Studies on the roles of human ferroportin and hephaestin in iron homeostasisThe thesis details methods for the recombinant expression, localization, and functional characterization of complex human membrane proteins (Ferroportin and Hephaestin) in Saccharomyces cerevisiae....
3 claims
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Study of Operational Strategies and Carbon Source Selection for the Production of Phytase using PichThe document is a detailed thesis on optimizing the operational strategies (feeding methods, carbon sources, promoter usage like pGAP) for the Pichia pastoris expression platform. Pichia is one of...
3 claims
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Study of glycerol-methanol and sorbitol-methanol mixed feed strategies for recombinant hPDGF-BB prodThe document provides detailed, practical fermentation strategies and scale-up data (7.5 L) focused on maximizing titer and secretion efficiency in the Pichia pastoris expression platform. Although...
2 claims
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Study of pH and Thermodynamic Parameters via Circular Dichroism Spectroscopy of a Recombinant Human2024
The document investigates the structural fidelity and stability of recombinant human lactoferrin, a target protein, produced in the specific expression platform Komagataella phaffii (Pichia). This...
3 claims
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Studying the effects of frequency and amplitude of periodic oxygen-related stress on recombinant proThe document focuses on optimizing recombinant protein production in Pichia pastoris (a key platform in the research goal) by manipulating fermentation parameters (oxygen stress, DO control). It...
3 claims
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Substrate feeding strategies in Pichia pastoris fed-batch cultivation processes Analysis of key paraThe document investigates detailed bioprocess strategies (substrate feeding, methanol control) to maximize recombinant protein titer and secretion rates in Pichia pastoris. Although the target...
3 claims
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Subtilisin-like serine protease gene <i>TghSS42< i> from <i>Trichoderma ghanense&lThe document utilizes and reports detailed construction and performance metrics (titer/activity, induction conditions) for one of the key expression platforms mentioned in the research goal: bacteria...
3 claims
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Surface display of p75, a Lactobacillus rhamnosus GG derived protein, on Bacillus subtilis spores an2020
The document details strain construction, expression strategies (surface display via CotG anchor), stability testing, and bioactivity measurement within a bacterial expression platform (Bacillus...
2 claims
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Sweet factory an O-glycosylation competent Escherichia coli strain for the recombinant expression of2018 · Prof. Dr.
The document details the engineering of an E. coli strain (a bacterial platform listed in the research goal) to achieve complex O-glycosylation (PTM fidelity), which is a critical challenge and...
3 claims
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Synergistic Antimicrobial Action of Lactoferrin-Derived Peptides and Quorum Quenching Enzymes2023 · Enzymes. Int.
The document investigates the bioactivity (antimicrobial efficacy) of key human milk protein derivatives, specifically lactoferrin-derived peptides (lactoferricin, lactoferampin). Evaluating...
2 claims
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Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase product2021
This document studies strain engineering in Pichia pastoris (a platform central to the research goal) aimed at optimizing secretion, folding, and titer. Specifically, it uses bidirectional promoters...
3 claims
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Synthesis of an Anti-CD7 Recombinant Immunotoxin Based on PE24 in CHO and E. coli Cell-Free Systems2022 · Systems. Int.
The document compares protein synthesis outcomes (yield and function/bioactivity) between a prokaryotic system (E. coli) and a eukaryotic system (CHO), which is fundamentally relevant to the research...
3 claims
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Synthetic Biology Tools for Engineering Aspergillus oryzae2024
The document focuses on engineering tools (promoters, signal peptides, DNA assembly) and strategies for enhancing heterologous protein expression and secretion in Aspergillus oryzae. A. oryzae is a...
3 claims
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Synthetic biology‐based heterologous expression and purification of enterocin A advancing antimicrobThe document investigates the heterologous expression, purification, yield (titer/inclusion bodies), and bioactivity of a bioactive peptide (Enterocin A) using a bacterial expression platform (E....
3 claims
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Synthetic phosphopeptides and phosphoproteins as tools for studying peptide and protein self-assemblProf. Dr.
The research goal requires detailed information on PTM fidelity (especially phosphorylation and glycosylation) of human milk proteins (HMPs) like caseins. This document details synthetic and...
3 claims
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Synthetic signal sequences that enable efficient secretory protein production in the yeast KluyveromThe research goal specifically requires detailed strain construction data, including promoters and signal peptides, for fungal expression platforms (Pichia, Saccharomyces). This document provides...
3 claims
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T4 DNA LIGASE DETERMINING THE OPTIMAL SYSTEM FOR INDUSTRIAL EXPRESSION AND PURIFICATIONThe document compares industrial expression and purification outcomes (yield, specific activity, cost) across multiple expression platforms (E. coli, B. megaterium, Baculovirus system). While the...
3 claims
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THE OBTAINING OF THE RECOMBINANT CAMEL CHYMOSIN BY SUBMERGE FERMENTATION IN THE PILOT BIOREACTORThe document provides specific, high-level engineering data (titer: 174.5 U/mL, secretion, pilot-scale fermentation in a 50L bioreactor) for the Pichia pastoris expression platform using a...
2 claims
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THE ROLE OF P24 PROTEINS IN THE ER TO GOLGI TRANSPORT OF TOLL-LIKE RECEPTORSThe document investigates fundamental mechanisms of protein secretion and post-translational modification (PTMs), specifically the role of conserved p24 cargo receptors (TMEDs) in ER-to-Golgi...
2 claims
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TRANSGALACTOSYLATION FOR GALACTOOLIGOSACCHARIDE SYNTHESIS USING PURIFIED AND CHARACTERIZED RECOMBINA5708
The document investigates the heterologous expression, purification, yield, and characterization of a recombinant protein (alpha-galactosidase) using a filamentous fungus (Aspergillus sojae), which...
3 claims
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Talactoferrin Immunotherapy in Metastatic Renal Cell Carcinoma A Case Series of Four Long-Term SurviThe document discusses Talactoferrin alfa, which is recombinant human lactoferrin (a target protein) purified from Aspergillus (a target filamentous fungi expression platform). Although the focus is...
1 claim
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Talaromyces marneffei Cu, Zn Superoxide Dismutase Recombinant Protein Expression in Pichia pastoris,2023
The document provides detailed, transferable technical data on strain construction (pPICzαB vector, alpha-factor signal peptide), successful protein secretion, and functional expression (enzymatic...
2 claims
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Targeted Amino-Terminal Acetylation of Recombinant Proteins in E. coli2010 · PLOS ONE
The research goal compares bacterial expression platforms (E. coli) regarding PTM fidelity, bioactivity, and titer. This document details a specific strain engineering technique (co-expression of...
3 claims
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Targeted Vaccination against Human α-Lactalbumin for Immunotherapy and Primary Immunoprevention of T2016
The document focuses on research using recombinant human alpha-lactalbumin (rhα-lactalbumin), which is a core target protein of the research goal. While the application is cancer immunology, the...
2 claims
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Techno-economic analysis of the industrial production of a low-cost enzyme using E. coli the case of2018
The document performs a techno-economic analysis (TEA) of recombinant protein production using one of the required platforms (E. coli/bacteria). While the target protein (beta-glucosidase) is...
3 claims
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Temperature Screening Of Trisaccharide (GlcNAc 1, 3Gal 1, 4Glc) Synthesis In Recombinant EscherichiaThe document details strain construction (plasmid, E. coli JM109) and the optimization of fermentation conditions (temperature screening, scale-up) to improve production yield (titer) in a bacterial...
3 claims
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The advent of plant cells in bioreactors2023 · Frontiers in Plant Science
The document reviews industrial bioprocessing using bioreactors for recombinant protein production (including scale-up, GMP, and yield optimization). These engineering and regulatory aspects are...
3 claims
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The Antifungal Activity of Lactoferrin and Its Derived Peptides Mechanisms of Action and Synergy wit2017 · Frontiers in Microbiology
The document focuses on the bioactivity (antifungal function) and mechanism of action of lactoferrin, which is one of the key bioactive human milk proteins targeted by the research goal. It...
2 claims
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The Antimicrobial Activity of Human Defensins at Physiological Non-Permeabilizing Concentrations Is2024 · Pases. Int.
The research goal focuses on the production and bioactivity of human milk proteins, including lactoferrin. This document directly investigates the antimicrobial mechanism (bioactivity) of human...
2 claims
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The Construction Of A Probiotic Lactic Acid Bacterium Expressing Acid-Resistant Phytase EnzymeThe document provides detailed strain construction data, expression outcomes, and stability analysis for heterologous protein expression (phytase) in bacterial platforms (Lactococcus/Lactobacillus),...
3 claims
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The Expression of Recombinant Human Serum Albumin in the Mammary Gland of Transgenic MiceThe document investigates detailed expression strategies, specifically utilizing the bovine αs1-casein promoter to achieve high-level secretion (titer) of a recombinant human protein. Although the...
3 claims
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The Functional Role of Lactoferrin in Intestine Mucosal Immune System and Inflammatory Bowel Disease2021 · Frontiers in Nutrition
The document details the functional role and required bioactivity of Lactoferrin (a key target human milk protein) regarding intestinal immunity and inflammation. While it does not cover expression...
2 claims
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The Impact of Lactoferrin on the Growth of Intestinal Inhabitant Bacteria2019
The research goal requires data on PTM fidelity (glycosylation) and bioactivity for human milk proteins like lactoferrin. This document provides critical reference information detailing the...
3 claims
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The LiaFSR three-component System of Bacillus subtilisProf. Dr.
The dissertation details the 'LIKE system,' a novel protein expression toolbox for Bacillus subtilis based on the liaI promoter (Chapter 3). This provides crucial data regarding strain construction...
3 claims
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The NMR signature of gluconoylation a frequent N-terminal modification of isotope-labeled proteins2019 · Journal of Biomolecular NMR
The research goal requires assessing PTM fidelity across different platforms, including bacteria (E. coli). This document characterizes N-terminal gluconoylation, a frequent PTM found specifically in...
2 claims
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The Potential for Sialic Acid and Sialylated Glycoconjugates as Feed Additives to Enhance Pig Health2021 · Veterinary Sciences
The research goal focuses on PTM fidelity (glycosylation) and bioactivity for infant nutrition regarding human milk proteins (e.g., lactoferrin). This document specifically reviews the essential...
3 claims
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The Promoter Toolbox for Recombinant Gene Expression in Trichoderma reesei2018 · Frontiers in Bioengineering
The document reviews the promoter toolbox for a relevant filamentous fungal expression platform (*Trichoderma reesei*). It directly addresses key elements of the research goal, including strain...
2 claims
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The Road from Host-Defense Peptides to a New Generation of Antimicrobial Drugs2018
The document, a review on Host-Defense Peptides (HDPs) and Antimicrobial Peptides (AMPs), explicitly mentions one of the target human milk proteins, lactoferrin (specifically the hLF1-11 fragment),...
2 claims
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The Role of Lactoferrin in Intestinal Health2023
The document defines the key biological function and required bioactivity (intestinal health, iron absorption, immunomodulation) of lactoferrin (a target protein) in infants. Understanding this...
3 claims
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The Role of Maternal Breast Milk in Preventing Infantile Diarrhea in the Developing World.2014
The document details the required bioactivity and clinical outcomes (protection against diarrhea) for key target proteins mentioned in the research goal (lactoferrin, secretory immunoglobulins). This...
2 claims
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Therapeutic potential of Camel MilkThe document, while focusing on camel milk, reviews the composition and therapeutic bioactivity (anti-cancer, antimicrobial) of key human milk proteins targeted by the research goal, including...
3 claims
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The Theoretical And Practical Aspects Of The Recombinant Antigens For The Diagnosis Of Bovine LeukemThe document compares the use of Saccharomyces cerevisiae (yeast) and E. coli (bacteria) for recombinant protein production, two platforms specified in the research goal. It provides concrete...
3 claims
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The Transcriptomic Mechanism of a Novel Autolysis Induced by a Recombinant Antibacterial Peptide fro2022
The document investigates optimization strategies (autolysis mechanism, transcriptomics, genetic modification) within a target expression platform (Pichia pastoris). Understanding and controlling...
3 claims
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The Use of α-MF Signal Peptide Without Spacer for Producing Insulin Aspart Precursor in i Pichia pasJournal of Biochemistry and Biotechnology
The document investigates detailed strain engineering outcomes, specifically optimizing protein secretion and titer in Pichia pastoris by manipulating the alpha-mating factor signal peptide (removing...
3 claims
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The acute phase protein lactoferrin is a key feature of Alzheimer’s disease and predictor of Aβ burd2021
The document investigates the specific molecular mechanisms and functional requirements (e.g., iron-bound form) for the bioactivity of lactoferrin. Although the context is Alzheimer's disease,...
2 claims
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The ancient koji mold (Aspergillus oryzae) as a modern biotechnological tool2021 · Bioresour. Bioprocess.
The document reviews Aspergillus oryzae, which is a filamentous fungus expression platform (required by the goal). It specifically mentions the use of A. oryzae for producing human lysozyme, one of...
3 claims
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The biophysical chemistry of frog foam nest proteins2017
This PhD thesis details the methodology for cloning and expressing a recombinant protein (Ranaspumin 2) using a bacterial expression platform, which is one of the required platforms for comparison in...
3 claims
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The concentration of recombinant human lactoferrin in the milk of transgenic goats of the third andThe document investigates recombinant human lactoferrin (rHLF), a target protein, and reports key metrics (titer/concentration up to 9 g/L) and engineering details (goat beta-casein promoter) in a...
3 claims
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The dual antimicrobial and immunomodulatory roles of host defense peptides and their applications in2022 · Journal of Animal Science and Biotechnology
The research goal focuses on bioactive human milk proteins, including lactoferrin and lysozyme. This document reviews the antimicrobial and immunomodulatory roles of host defense peptides (HDPs),...
2 claims
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The dynamic behavior of storage organelles in developing cereal seeds and its impact on the producti2014
The document discusses the production of recombinant proteins using molecular farming in cereals, specifically addressing two of the research goal's target proteins: lactoferrin and lysozyme. It...
2 claims
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The effect of carbohydrate moiety structure on the immunoregulatory activity of lactoferrin in vitro2013
The document directly addresses the production of a target human milk protein (lactoferrin) using one of the specified expression platforms (glycoengineered Pichia pastoris). It specifically compares...
3 claims
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The effect of process conditions on productivity and glycosylation of cystatin C in P. pastoris andThe document investigates productivity and glycosylation optimization in two key expression platforms (P. pastoris and S. cerevisiae) specified in the research goal. The detailed findings on process...
3 claims
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The engineered expression of secreted HSPB5-Fc in CHO cells exhibits cytoprotection in vitroThe document discusses the expression, secretion, and post-translational modification (N-linked glycosylation) of a recombinant protein. Although the platform is CHO cells (mammalian), the findings...
3 claims
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The evaluation of the factors that cause aggregation during recombinant expression in E. coli is sim2006
The document investigates optimization strategies (testing temperature, vectors, co-expression, osmolytes) and develops tools for assessing solubility and yield (titer) in E. coli, which is one of...
2 claims
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The expression, purification and characterization of recombinant human lung surfactant protein-BAlthough the target protein is Lung Surfactant Protein-B (SP-B) rather than a milk protein, this PhD thesis provides detailed, high-quality data highly relevant to the research goal. It specifically...
3 claims
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The expression of cellulomomas fimi cellulase genes in Brevibacterium lactofermentum and characterizThe document is a PhD thesis providing detailed strain engineering data (vector construction, promoter/regulatory sequence analysis, signal peptide/secretion analysis via leader sequence...
3 claims
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The goat β-casein CMV chimeric promoter drives the expression of hLF in transgenic goats produced byThe document focuses on producing one of the target proteins (lactoferrin) and provides critical benchmark data (titer, confirmed bioactivity, specific expression components like signal peptides)...
3 claims
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The immunomodulatory effect of plant lectins a review with emphasis on ArtinM properties2013
Although the document focuses on plant lectins (ArtinM, ML), it discusses the heterologous expression of recombinant lectins (rML) and provides detailed findings on how PTM fidelity (specifically...
2 claims
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The iron uptake mechanisms of enteroinvasive Escherichia coli2000 · Journal of Microbiology
The document investigates iron uptake mechanisms in the bacterial expression platform *E. coli* and explicitly utilizes human lactoferrin (HLf), a key target protein in the research goal, as a probe...
1 claim
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The pCri System A Vector Collection for Recombinant Protein Expression and Purification2014 · PLOS ONE
The document describes a vector system designed for high-throughput comparison of recombinant protein expression and purification across multiple host platforms directly relevant to the research...
3 claims
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The pET expression system revisited - how to boost soluble recombinant protein expression in E. coliPrivatdoz. Dipl.
Document focuses on optimizing soluble recombinant protein expression (titer and folding/solubility metrics) using the pET system in E. coli, which is one of the required bacterial expression...
3 claims
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The potential of lactoferrin, ovotransferrin and lysozyme as antiviral and immune-modulating agentsThe document extensively reviews the bioactivity (antiviral and immunomodulatory properties, iron homeostasis) of key target proteins (lactoferrin and lysozyme) mentioned in the research goal....
2 claims
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The protein structure of recombinant human lactoferrin produced in the milk of transgenic cows close2005
The document provides a detailed comparison of Post-Translational Modification (PTM) fidelity (specifically N-linked glycosylation) and structural integrity of recombinant lactoferrin against the...
3 claims
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The purification and characterization of two cellulose-binding, glycosylated cellulases from the bacThe document provides detailed outcomes concerning one of the required expression platforms (bacteria, specifically Cellulomonas fimi, with comparison to E. coli recombinant production). Its central...
3 claims
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The recombinant l-lysine α-oxidase from the fungus Trichoderma harzianum promotes apoptosis and necr2024
The document investigates the recombinant expression and characterization of a fungal protein (L-lysine α-oxidase) produced in E. coli. While the specific protein is not a target human milk protein,...
2 claims
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The supramolecular chemistry of natural sweetenersThe document discusses the establishment and engineering of a recombinant expression system for a complex, secreted, disulfide-bonded protein (thaumatin), specifically focusing on 'Engineering of...
3 claims
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The use of fungi for the production of biotechnological products in the field of healthThe document is a review focusing on the use of fungal expression platforms (Saccharomyces, other fungi/molds) for producing heterologous biotechnological products, including pharmaceutical proteins....
3 claims
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The α-mating factor secretion signals and endogenous signal peptides for recombinant protein secreti2022
The document systematically evaluates the efficiency of various signal peptides (a key component of strain construction) for optimizing recombinant protein secretion and titer in Komagataella phaffii...
2 claims
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Three-dimensional culture of chicken primordial germ cells (cPGCs) in defined media containing the f2018 · PLOS ONE
The document, while focusing on avian cells (cPGCs), provides explicit comparisons with bacteria (E. coli) and yeast (Saccharomyces, Pichia pastoris) regarding their limitations in Post-Translational...
3 claims
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Tobacco, a highly efficient green bioreactor for production of therapeutic proteins2009
Although the primary expression platform discussed (Tobacco/Plants) is not one of the microbial systems specified (Pichia, Saccharomyces, bacteria), the document provides transferable comparative...
3 claims
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Tobacco BY2 cells expressing recombinant cardosin B as an alternative for production of active milk2021
The document contains critical comparative performance data for two expression platforms specifically named in the research goal (Pichia pastoris and Saccharomyces cerevisiae). It discusses issues...
2 claims
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Towards phototrophic tissue engineering Optimization of a biotechnology platform for human recombinaProf. Dr.
Although the platform (Chlamydomonas reinhardtii) and the expressed proteins (human growth factors) are outside the primary scope (yeast/fungi/bacteria expressing human milk proteins), the document...
3 claims
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Towards solid-state NMR spectroscopic studies of the ETBR ET-1 complexThe document investigates heterologous protein expression using platforms central to the research goal, specifically detailing methods for optimization and purification using Pichia pastoris (Chapter...
3 claims
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Transcriptome Atlas of 16 Donkey Tissues2021 · Frontiers in Genetics
The research goal focuses on the recombinant production quality of specific human milk proteins, including lysozyme. This document provides quantitative proteomics and transcriptome data...
3 claims
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Toxicological Evaluation of Lactase Derived from Recombinant Pichia pastoris2014 · PLOS ONE
The document, while focusing on lactase instead of specific human milk proteins (HMPs), uses one of the required expression platforms (Pichia pastoris) and addresses multiple key metrics relevant to...
3 claims
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Transferrins Reduce Replication of Chlamydia suis in McCoy Cells2021 · Veterinary Sciences
The research goal requires assessing the bioactivity of produced human milk proteins, specifically lactoferrin (LF). This document analyzes the antimicrobial bioactivity of bovine lactoferrin (bLF)...
2 claims
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Transgene Mapping in Animals What to Choose2025 · Mol. Sci.
Although focused on transgenic animals, the review details essential molecular methods (NGS, PCR, long-read sequencing) for mapping random transgene integration sites. This methodology is critical...
1 claim
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Transgenic Plants as Biofactories for the Production of Biopharmaceuticals A Case Study of Human PlaThe document compares the performance (PTM fidelity, secretion, yield, cost) and limitations of several platforms listed in the research goal (bacteria/E. coli and yeast systems) against mammalian...
3 claims
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Transgenic expression of lactoferrin imparts enhanced resistance to head blight of wheat caused by FThe document focuses on the expression and functional quantification (titer and antifungal bioactivity) of lactoferrin, a key target protein class. Although the expression platform (wheat) is outside...
3 claims
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Transgenic tobacco plants expressing antimicrobial peptide bovine lactoferricin show enhanced resist2012
The document provides detailed genetic construction strategies (promoter choice, signal peptide fusion, chimeric gene synthesis) for expressing and achieving bioactivity of a human milk protein...
2 claims
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Transient Expression of Secretory IgA In Planta is Optimal Using a Multi-Gene Vector and may be Furt2016 · Frontiers in Plant Science
The document focuses entirely on one of the key target proteins (secretory IgA, sIgA) and evaluates critical parameters listed in the research goal, namely titer (yield optimization), secretion, and...
3 claims
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Transient Glyco-Engineering to Produce Recombinant IgA1 with Defined N- and O-Glycans in Plants2016 · Frontiers in Plant Science
The document focuses on the production and glyco-engineering (PTM fidelity) of recombinant sIgA1, which is one of the target human milk proteins in the research goal. While the expression platform is...
3 claims
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Transport processes in currently used bioreactors - [kumulative Dissertation]Prof. Dr.
The document investigates critical bioprocess engineering parameters (mass transfer, kLa, mixing, shear stress, Pluronic effects) necessary for scaling up bioproduction in large bioreactors (1-25...
2 claims
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Triggering the Expression of Cellulolytic Genes Using a Recombinant Endoxylanase from Trichoderma haThe document details the heterologous expression of a protein in Pichia pastoris (a key platform) and provides specific methods and findings concerning Post-Translational Modification (PTM) fidelity....
1 claim
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Tunable recombinant protein expression in E. coli enabler for continuous processing2016
The document focuses entirely on optimizing recombinant protein production (titer, quality, folding, secretion/translocation, mitigating metabolic burden) within one of the target expression...
2 claims
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Tunable recombinant protein expression in E. coli promoter systems and genetic constraints2016
The document provides a detailed review of promoter systems and genetic constraints for optimizing tunable recombinant protein expression in E. coli, directly addressing the 'bacteria' expression...
2 claims
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Two Distinct C-Type Lysozymes in Goldfish Molecular Characterization, Antimicrobial Potential, and T2020
The document investigates the molecular characterization and antimicrobial potential of a C-type lysozyme (a target human milk protein) produced recombinantly. Crucially, it specifically uses the E....
2 claims
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Un nouveau clone et une nouvelle méthode pour la production et la purification de l’entérotoxine STbAlthough the target protein is STb enterotoxin, the document provides detailed, high-quality methodological information specifically requested by the research goal: strain construction data...
3 claims
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Understanding exopolysaccharide byproduct formation in Komagataella phaffii fermentation processes f2024
The document investigates a critical issue (exopolysaccharide impurity) associated with high cell density fermentation in Komagataella phaffii (Pichia pastoris), which is a key expression platform in...
2 claims
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Unlocking the power of antimicrobial peptides advances in production, optimization, and therapeutics2025 · Frontiers in Cellular
The document, while focused on general Antimicrobial Peptides (AMPs), directly addresses the core methods and challenges of the research goal. It reviews the optimization of production using...
3 claims
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Unravelling elusive mysteries of bovine milk proteins by mass spectrometryThe research goal requires evaluating the PTM fidelity (especially glycosylation) of recombinant human milk proteins. This document provides detailed mass spectrometry analysis of the native PTMs...
3 claims
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Untersuchungen an einem zellfreien Proteinsynthesesystem basierend auf S30 Extrakten von EscherichiaProf. Dr. Dr.
The document is a PhD thesis providing detailed optimization strategies and methodological data for a bacterial expression platform (E. coli cell-free system). This directly contributes to...
3 claims
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Untersuchungen der Populationsheterogenität während der heterologen Proteinproduktion in Bacillus me2015
The document investigates fundamental limitations (population heterogeneity, plasmid stability/PCN, promoter control, single-cell dynamics) affecting high-titer production in a relevant expression...
3 claims
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Untersuchungen zu Transglykosylierungsreaktionen von Glykosidasen und Glykosynthasen für die Synthes2014 · Journal of Biotechnology
The document focuses on the synthesis and engineering of N-acetyllactosamine (LacNAc) glycans using engineered glycosidases (glycosynthases) and reaction engineering. LacNAc structures are crucial...
3 claims
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Untersuchungen zur Biosynthese und Funktion von Myxochelinen in MyxobakterienProf. Dr.
Although the document studies the biosynthesis of Myxochelin siderophores, it provides detailed, practical information on strain construction and engineering outcomes for a bacterial expression...
3 claims
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Untersuchungen zur Synthese von Biopolymeren unter Einsatz von Biokatalysatoren in parallelen Multie2015 · Prof. Dr.
The document, a PhD thesis on biopolymer synthesis using biocatalysts, focuses heavily on N-glycosylation (N-Glykosylierung) and enzymatic synthesis of glycosyl structures (e.g., GDP-Mannose,...
3 claims
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Untersuchungen zur XylR-vermittelten Regulation des Xylose-Operons und phänotypischen Heterogenität2015
The document investigates the XylR-inducible expression system and phenotypic heterogeneity specifically during heterologous protein production in Bacillus megaterium. Bacillus megaterium is a...
3 claims
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Untersuchungen zur optimalen Kultivierung von Leishmania tarentolaeProf. Dr.
The document focuses on optimizing the cultivation (titer/secretion) and defining media for a eukaryotic expression platform (*Leishmania tarentolae*). Although *L. tarentolae* is not one of the...
3 claims
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Untersuchungen zur rekombinanten Proteinproduktion und Tetrapyrrolbiosynthese in Bacillus megaterium2015
The document investigates the optimization and engineering of a bacterial expression platform (Bacillus megaterium) regarding recombinant protein production, focusing on titer, secretion efficiency,...
3 claims
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Upscale of recombinant α-L-rhamnosidase production by Pichia pastoris MutS strain2015 · Frontiers in Microbiology
The document optimizes the Pichia pastoris expression platform (a core platform in the research goal) focusing on maximizing titer and secretion through fed-batch fermentation protocols (DO...
2 claims
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Use of folding modulators to improve heterologous protein production in Escherichia coliThe document reviews strain engineering strategies (folding modulators, chaperones) to improve functional production, titer, and solubility of heterologous proteins in E. coli, one of the target...
1 claim
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Using microorganisms culture supernatants to supply enzymes to biofuel cells and extend cathode lifeDipl. Biol.
The document compares protein expression and secretion performance across the key microbial platforms (bacteria, yeast, filamentous fungi) listed in the research goal (E. coli, Y. lipolytica, T....
3 claims
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VIRAL ION CHANNEL PRODUCTION FOR STRUCTURAL STUDIESThe document focuses on producing viral ion channels, not human milk proteins. However, it provides detailed, platform-specific production methodologies (Materials and Methods) for key expression...
3 claims
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Variability in immune-active human milk components2020
The document investigates the native structural characteristics (N-glycans/PTMs, Chapter 4) and stability/digestion kinetics (bioactivity, Chapter 3) of the exact target human milk proteins...
3 claims
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Venoma da cascavel brasileira Crotalus durissus terrificus e caracterização de um inibidor recombinaProfa. Dra.
The document, although focused on a snake venom protein inhibitor (PLI), directly addresses the comparison of key expression platforms (Pichia pastoris and E. coli). Crucially, it provides detailed...
2 claims
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Verwendung der einzelligen Grünalge Chlamydomonas reinhardtii als Expressionssystem für Synthese undProf. Dr.
The document, a PhD thesis on recombinant protein expression in Chlamydomonas reinhardtii (algae), is not one of the target platforms (yeast, fungi, bacteria), nor does it use the target proteins...
3 claims
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Veteriner İlaç Alanında Rekombinant DNA Teknolojisinin KullanımıSag. Bil. Enst. Derg.
The document reviews recombinant DNA technology, which is the core methodology required by the research goal. Although it focuses on veterinary applications and transgenic animals, it specifically...
3 claims
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Vesicle-based cell-free synthesis of short and long unspecific peroxygenasesFrontiers in Bioengineering
The document, while focusing on fungal UPOs and cell-free synthesis (CFPS), provides significant background context relevant to the research goal. It specifically discusses the challenges and...
2 claims
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Virus-like particle production with yeast Ultrastructural and immunocytochemical insights into PichiThe document investigates key aspects of the research goal within the specified expression platforms (Pichia pastoris, Saccharomyces cerevisiae): titer (high level production), secretion (ER...
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WITHDRAWN Secretion of IFN-γ by transgenic mammary epithelial cells in vitro reduced mastitis infectAlthough the expression platform (goat mammary epithelial cells) is not one of the target microbial platforms (Pichia, Saccharomyces, bacteria), the document provides detailed strain engineering data...
2 claims
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WITHDRAWN Secretion of IFN-γ in goat mammary epithelial cells reduce the mastitis infection riskThe document focuses on gene expression, protein secretion (using a casein promoter), and bioactivity testing (anti-bacterial assays), which are key metrics in the research goal. Although the...
2 claims
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Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a pigletThe document directly addresses several key aspects of the research goal: it uses a specified expression platform (Pichia), produces a human milk protein type (IgA/sIgA is listed in the goal), and...
3 claims
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Yeast Expressed Hybrid Peptide CLP Abridged Pro-Inflammatory Cytokine Levels by Endotoxin Neutraliza2023
The document focuses on the high-level heterologous expression and secretion of a peptide using Pichia pastoris, which is one of the key expression platforms mentioned in the research goal. It...
2 claims
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Yeast Surface Display of Two Proteins Previously Shown to Be Protective Against White Spot Syndrome2015 · PLOS ONE
The document utilizes two key expression platforms specified in the research goal (Pichia pastoris and Saccharomyces cerevisiae) and details specific strain engineering approaches, including promoter...
2 claims
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Yeast expressed recombinant Hemagglutinin protein of Novel H1N1 elicits neutralising antibodies in rThe document provides a detailed comparison between Pichia pastoris and bacterial expression systems regarding protein secretion, titer enhancement (multi-copy integration), and fidelity of...
3 claims
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Zinc-finger nickase-mediated insertion of the lysostaphin gene into the beta-casein locus in cloned2013
Although the document focuses on transgenic cows (mammalian platform) rather than microbial platforms, it specifically details genetic engineering targeting the beta-casein (CSN2) locus, which is a...
2 claims
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cDNA Cloning of a Bovine Insulin-like growth factor-1 from Egyptian Buffalos and Expression of its RArq. Bras. Med. Vet. Zootec.
The document investigates the expression of a recombinant protein (Bovine IGF-1) in one of the required platforms (Escherichia coli/bacteria). It provides critical metrics necessary for the research...
3 claims
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i Pichia pastoris i — recombinant enzyme producent for environment treatment — reviewThe document extensively details the advantages and characteristics of Pichia pastoris, one of the primary expression platforms defined in the research goal. It provides transferable information...
3 claims
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pH Dependent Antimicrobial Peptides and Proteins, Their Mechanisms of Action and Potential as Therap2016
The document reviews the bioactivity and mechanisms of action (including pH dependence and metal ion binding) of lactoferrin, one of the primary human milk proteins targeted by the research goal....
3 claims
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pTSara-NatB, an improved N-terminal acetylation system for recombinant protein expression in E. coli2018 · PLOS ONE
The document details strain construction and engineering outcomes for improving Post-Translational Modification (PTM) fidelity (N-terminal acetylation) in the bacterial expression platform (E. coli)....
1 claim
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Μοριακή και βιοχημική ανάλυση του γονιδίου β-γλυκοσιδάση της ολευρωπαΐνης στην ανάπτυξη και άμυνα τηAlthough the document focuses on olive tree β-glucosidase, it is a PhD thesis detailing the methodology for heterologous expression in E. coli (a target expression platform) and involves transferable...
1 claim
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‘Mother(Nature) knows best’ – hijacking nature-designed transcriptional programs for enhancing stres2024
The document investigates strain engineering strategies (hijacking transcriptional programs/overexpressing TFs) in yeast (Yarrowia lipolytica, referencing Pichia and Saccharomyces) specifically to...
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| Document | Claims |
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First of all I would like to express sincere gratitude to my supervisor, Prof. Ning Li, for his continuous guidance, support and encouragement throughout this study. I would like to thank Dr. Yurong Bi, who conducted the TEM experiments. I would like to express my appreciation to Waishing WongHong Kong University Of Science And Technology Hkust
The research goal focuses on expression platforms and human milk proteins, specifically caseins. Although the main topic is Arabidopsis development, the document details the 'Expression and...
1 claim
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UC Davis Electronic Theses and DissertationsUniversity Of California Davis
The document focuses on the natural regulation and gene expression of alpha-lactalbumin (a key target protein listed in the goal) in mammalian systems. Chapters detailing the genetic regulation of...
2 claims
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A New Sugarcane Cystatin Strongly Binds to Dental Enamel and Reduces ErosionUniversity Of Cambridge
The document utilizes one of the target expression platforms, Escherichia coli (bacteria), for recombinant protein production. Furthermore, the functional evaluation section explicitly uses and...
3 claims
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Native-State Stability Determines the Extent of Degradation Relative to Secretion of Protein Variants from Pichia pastoris2011 · University Of Cambridge
The document directly studies the expression, secretion, and yield optimization of human lysozyme (a target human milk protein) in Pichia pastoris (a target expression platform). It provides detailed...
3 claims
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Development of a Platform for Vectorized Expression of Secretory IgA2025 · University Of Guelph
The document focuses on detailed construct design, expression, and assembly challenges of Secretory IgA (sIgA), a key target protein. Although the expression platform (AAV/mammalian cells) is not...
3 claims
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Transcriptome and network analyses in Saccharomyces cerevisiae reveal that amphotericin B and lactoferrin synergy disrupt metal homeostasis and stress responseUnsw Sydney
The document performs transcriptome and network analysis in Saccharomyces cerevisiae (a target expression platform) studying its interaction with lactoferrin (a target protein). While the focus is on...
2 claims
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Antibiotic-Driven Dysbiosis Mediates Intraluminal Agglutination and Alternative Segregation of Enterococcus faecium from the IntestinalUtrecht University
The document investigates the specific bioactivity (agglutination and immune exclusion) of secretory IgA (sIgA), which is a core target protein listed in the research goal. Understanding the...
3 claims
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J. Dairy Sci. 99:5690–5700Utrecht University
The document investigates the natural bioactivity of lactoferrin and lactoperoxidase in bovine milk against common bacteria (S. aureus, E. coli). While the context is bovine mastitis, the methods...
2 claims
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Full Terms & Conditions of access and use can be found at2017 · Utrecht University
The research goal explicitly targets sIgA (Secretory IgA) production and its PTM fidelity (glycosylation) and bioactivity. This document focuses on generating, characterizing, and comparing the...
3 claims
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Outer membrane permeabilization by the membrane attack complex sensitizes Gram- negative bacteria to antimicrobial proteins in serum and phagocytesUtrecht University
The document investigates the bioactivity and mechanism of action of human lysozyme (a key target human milk protein listed in the research goal) against Gram-negative bacteria (E. coli)....
3 claims
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Outer membrane permeabilization by the membrane attack complex sensitizes Gram- negative bacteria to antimicrobial proteins in serum and phagocytesUtrecht University
The research goal requires assessing the bioactivity of human milk proteins (HMPs), including lysozyme. This document specifically investigates the mechanism and effectiveness of human lysozyme (an...
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| Document | Claims |
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US-10047134-B22018
The document, a patent, focuses on optimizing expression and secretion (titer and degradation control) in Pichia pastoris, which is one of the key expression platforms specified in the research goal....
3 claims
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US-10053682-B22018
The document provides detailed strain construction data (signal peptide removal, site-directed mutagenesis, recombinant vector design) and utilizes key expression platforms relevant to the goal...
3 claims
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US-10059777-B22018
The document focuses on the Secretory Component (SC), a core component of sIgA (a target human milk protein). While the platform used (CHO cells) is mammalian and not explicitly listed in the goal...
3 claims
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US-10100343-B22018
This patent provides detailed strain construction data for Pichia pastoris, a key expression platform in the research goal. It focuses specifically on engineering the strain to improve fermentation...
3 claims
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US-10131927-B22018
The document provides specific, patent-level data on optimizing the Pichia pastoris expression platform, which is one of the key platforms of interest. It details strategies for achieving high yield...
3 claims
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US-10329572-B22019
The document details specific strain engineering modifications in Pichia pastoris (a key platform) aimed at improving Post-Translational Modification (PTM) fidelity, specifically N-glycosylation...
3 claims
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US-10428123-B22019
The document is a patent detailing strain construction data (novel promoter, signal peptides) specifically optimized for high titer and secretion in key expression platforms relevant to the research...
3 claims
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US-10457970-B22019
The document details strain engineering methods (mannosyl transferase modification/knockout, specifically Alg3) in a eukaryotic microbial expression system (Labyrinthulomycetes) to achieve...
2 claims
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US-10533045-B22020
The document details processes (fermentation, purification, monitoring) for controlling and minimizing glycosylated impurities (PTM fidelity, glycovariants) in key expression platforms relevant to...
3 claims
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US-10584151-B22020
Although the recombinant protein is a tick vaccine antigen, the document provides specific details (codon optimization, synthetic gene design, expression in Pichia pastoris) relevant to strain...
2 claims
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US-10612033-B22020
The document directly addresses engineering the Pichia pastoris expression platform (one of the specified platforms) to improve Post-Translational Modification (PTM) fidelity, specifically...
3 claims
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US-10619164-B22020
The document provides detailed strain construction data (novel constitutive promoters) and methods specifically for the Pichia pastoris expression platform. Pichia is a key platform targeted by the...
3 claims
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US-10676750-B22020
The document provides detailed strain construction data (novel methanol-inducible promoters, expression vectors, host cells) specifically for Pichia pastoris, which is one of the target expression...
3 claims
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US-10975128-B22021
This patent provides detailed, transferable strain engineering data for one of the target expression platforms, Pichia pastoris. It discusses codon optimization, specific vectors (pPICZαA, pPIC3.5K),...
3 claims
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US-11041163-B22021
The document addresses a core challenge of the research goal: ensuring PTM fidelity (specifically controlling N-glycosylation) in eukaryotic expression systems like yeast. Although the documented...
2 claims
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US-10982246-B22021
The document details specific, transferable strain engineering outcomes for the key expression platform Pichia pastoris, including achieving high constitutive titer, high gene copy numbers, and...
3 claims
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US-11155801-B22021
The document provides highly specific, transferable data relevant to the research goal's metrics and platforms. It details the production of a protein (Lysostaphin) in Pichia pastoris, comparing...
3 claims
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US-11168117-B22021
The patent details the construction and use of a novel strong constitutive Pichia pastoris promoter system (pCS1 variant) designed to increase expression levels (titer) compared to standard promoters...
3 claims
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US-11220692-B22022
The document provides detailed strain construction data (mutant OCH1 allele, alpha-1,2-mannosidase expression) for engineering Pichia pastoris—one of the key expression platforms—to achieve...
3 claims
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US-11236137-B22022
The document provides highly detailed strain construction data, gene optimization strategies (codon optimization for Pichia), and expression methods (specific vectors, strains, purification...
3 claims
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US-11319353-B22022
Although the target protein (Der p2) is not a human milk protein, the document is a patent providing highly detailed strain construction data, codon optimization strategies, specific vector/strain...
3 claims
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US-11352610-B22022
Although the document focuses on optimizing lipid (TAG) production using heterologous DGA1 in the oleaginous yeast Yarrowia lipolytica, it provides detailed strain engineering data, specific...
3 claims
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US-11359191-B22022
The document provides detailed strain construction data (codon optimization, specific vectors/strains, use of alpha-factor signal peptide, activating elements) and engineering outcomes regarding...
3 claims
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US-11673926-B22023
The document, a patent providing detailed molecular engineering methods (signal peptides, promoters, ER retention sequences, co-expression strategies) focuses intensively on controlling and altering...
3 claims
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US-11787852-B22023
The document, a patent, details the production and optimization of a recombinant human protein (Type XVII collagen) using one of the primary target expression platforms, Pichia pastoris. It...
3 claims
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US-11866715-B22024
The document provides detailed strain engineering data for Pichia pastoris specifically aimed at controlling and optimizing N-glycosylation (PTM fidelity). Controlling glycosylation is a critical...
3 claims
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US-11840717-B22023
The document is a patent providing detailed strain construction and engineering data (promoters, kinase co-expression) specifically targeting bioactive human milk proteins (caseins,...
2 claims
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US-12037632-B22024
The document, while focused on cellulase, details methods and vectors for optimizing protein secretion and expression titer in a relevant host (filamentous fungus, Trichoderma reesei). It provides...
3 claims
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US-11873523-B22024
The document provides detailed strain construction data (exogenous promoters, gene modification/deletion) and optimization strategies for titer improvement within Aspergillus, which is a target...
3 claims
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US-12049653-B22024
The document is a patent detailing strain engineering and vector design in a specific required expression platform (Pichia pastoris). Although the target protein is lysostaphin (not a human milk...
3 claims
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US-12264319-B22025
The research goal requires detailed strain construction data (promoters, signal peptides) for the Pichia expression platform. This patent focuses entirely on characterizing and utilizing novel...
3 claims
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US-12270022-B22025
The document is a patent providing highly specific, proprietary strain engineering data (mutations, targeting of growth factors, and vacuolar proteases) for the Pichia pastoris expression system,...
3 claims
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US-12312625-B22025
Although the target product is a small molecule (cordycepin), the document provides crucial, detailed, and transferable data regarding expression platform comparison and strain construction, which...
3 claims
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US-12365715-B22025
The document, a patent, details advanced protein engineering (fusion protein, linker sequences, specific N-glycosylation site additions) aimed at achieving biological activity and PTM fidelity for a...
3 claims
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US-12359211-B22025
The document is a patent detailing genetic engineering in Pichia pastoris (a target expression platform) specifically to achieve substantially homogeneous N-glycans. This directly addresses the...
3 claims
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US-12428657-B22025
The document focuses on Human Milk Oligosaccharides (HMOs) rather than proteins, but it utilizes one of the specified expression platforms (bacteria/E. coli) and provides highly detailed strain...
2 claims
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US-2002004048-A12002
The document, although focused on HCV proteins, provides detailed strain engineering and methodological information directly relevant to the research goal's metrics and platforms. Specifically, it...
3 claims
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US-2002044989-A12002
This patent specifically claims compositions and methods utilizing recombinant human milk proteins (lactoferrin, lysozyme, alpha-lactalbumin, caseins, sIgA) for infant nutrition. Although the excerpt...
3 claims
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US-2002034751-A12002
The document describes the production of a recombinant protein (AFAP1) involving Aspergillus fumigatus, which belongs to the class of filamentous fungi—one of the key expression platforms specified...
3 claims
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US-2002115628-A12002
The research goal specifically requires comparison based on PTM fidelity, particularly glycosylation. This patent provides detailed genetic and functional information (sequences, expression vectors,...
3 claims
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US-2002137134-A12002
This patent details specific strain engineering methods (gene knockouts, enzyme targeting, use of signal peptides) to achieve human-like glycosylation (PTM fidelity) in key expression platforms...
3 claims
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US-2003040040-A12003
The document focuses on the expression system design (promoters, signal peptides, vectors, specific strains) for Bile Salt-Stimulated Lipase (BSSL), a major bioactive human milk protein, using Pichia...
3 claims
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US-2003064437-A12003
The document provides highly detailed strain engineering data for one of the key expression platforms (Pichia pastoris) specified in the research goal. It directly addresses key metrics such as titer...
3 claims
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US-2003092160-A12003
The document, while focusing on mammalian expression systems (human cells), provides detailed context regarding the key challenge specified in the research goal: PTM fidelity (glycosylation). It...
3 claims
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US-2003104607-A12003
The document provides highly detailed strain construction data, specific promoters (AOX1, GAP), and secretion signal sequences (S. cerevisiae MF alpha) used in the Pichia pastoris expression...
3 claims
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US-2003119109-A12003
The document provides detailed, transferable technical information regarding the optimization of a key expression platform specified in the research goal (*Pichia pastoris*). It discusses...
3 claims
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US-2003140363-A12003
The document provides detailed strain construction data, specifically focusing on the promoter and signal peptide-encoding region (Claim 1(g)) derived from the lysozyme gene, which is one of the...
3 claims
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US-2003215920-A12003
The document provides detailed strain construction data (promoters and vectors) for heterologous gene expression in Pichia pastoris, which is one of the specified expression platforms. It focuses on...
2 claims
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US-2004091971-A12004
Although the target protein (Plasmodium AMA-1) is not a human milk protein, the document details critical strain engineering strategies in a key platform specified in the goal (Pichia pastoris). It...
3 claims
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US-2003211597-A12003
The document focuses on engineering yeast expression platforms (Saccharomyces and Hansenula/Pichia) to control Post-Translational Modifications (PTMs), specifically by using 'glycosylation minus...
3 claims
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US-2004106783-A12004
The document provides detailed strain construction data (isolation of a novel promoter/terminator system—ICL promoter—and its regulatory characteristics) for a key expression platform specified in...
2 claims
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US-2004166560-A12004
The document provides detailed strain construction data (vectors, AOX1 promoter, alpha-factor signal peptide) and secretion outcomes using two primary expression platforms specified in the research...
3 claims
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US-2004175798-A12004
The document is a patent detailing process engineering (continuous fermentation, constitutive promoter GAPDH, high titer) and PTM fidelity (high-mannose structure, comparison to Saccharomyces/lack of...
3 claims
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US-2004203095-A12004
The document, a patent, details specific strain construction and expression optimization methods (signal peptides, propeptide cleavage control, culture pH optimization, codon usage) critical for...
3 claims
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US-2004265954-A12004
The document details a strain engineering approach (introducing N-glycosylation pathways) into prokaryotic expression platforms (E. coli/bacteria), which is one of the platforms listed in the...
2 claims
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US-2004229341-A12004
The document details strain construction, expression vectors (promoters, secretion signal sequences), and method development in two of the key expression platforms specified in the research goal:...
3 claims
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US-2005089843-A12005
The document investigates the expression of glycoproteins in 'lower eukaryotes' (which includes Pichia and Saccharomyces, key platforms in the research goal) and focuses heavily on the resulting PTM...
3 claims
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US-2005114911-A12005
Although the document describes production in a transgenic mammalian system (swine milk) rather than the specified microbial systems (Pichia, Saccharomyces, bacteria), it focuses on a key target...
3 claims
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US-2005164386-A12005
The document provides detailed strain construction data (PTM enzyme integration under heterologous promoters) focused entirely on improving post-translational modification (sialylation/glycosylation...
3 claims
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US-2005203049-A12005
The research goal specifically requires optimizing PTM fidelity, focusing on glycosylation, when expressing human milk proteins in microbial systems. This patent provides detailed molecular...
1 claim
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US-2005287628-A12005
The document details strain engineering methods (introducing complex N-glycosylation pathways) into a bacterial expression platform (E. coli). This directly addresses the research goal's focus on...
3 claims
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US-2005229261-A12005
Although the primary expression platform (transgenic animals) and target protein (Factor VIII) are outside the scope, the document provides detailed strain construction data (specific DNA...
3 claims
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US-2006078963-A12006
The document provides detailed strain engineering methods and outcomes (glycosylation pathway modification, PTM fidelity) for key expression platforms (yeast, Pichia, filamentous fungi) identified in...
3 claims
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US-2006099688-A12006
The research goal requires detailed data on PTM fidelity and strain engineering outcomes across different expression platforms (yeast, bacteria). This patent provides detailed genetic information and...
3 claims
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US-2006148680-A12006
Although the document focuses on plant expression of human growth hormone (not HMPs), it provides detailed methodology on key transferable research topics: engineering signal peptides and...
3 claims
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US-2006148035-A12006
The document focuses on engineering lower eukaryotes (including Pichia pastoris, a target expression platform) to achieve human-like N-glycosylation profiles. This directly addresses the research...
3 claims
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US-2006211084-A12006
The document focuses on detailed strain construction and engineering outcomes (promoter systems, host optimization, achieving active enzyme via PTM/cofactor incorporation) specifically in Pichia...
3 claims
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US-2006177898-A12006
The document details methods for engineering the glycosylation pathways of relevant expression platforms (lower eukaryotes, specifically Pichia, other yeast, and filamentous fungi) to produce...
3 claims
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US-2006247425-A12006
The research goal focuses heavily on Post-Translational Modification (PTM) fidelity, specifically glycosylation, for producing bioactive human milk proteins (HMPs), including sIgA (an...
2 claims
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US-2007105127-A12007
The document provides detailed strain construction data (targeting signal peptides, promoters, exogenous enzymes) and engineering strategies required to modify fungal and yeast expression platforms...
3 claims
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US-2007178551-A12007
The document provides detailed strain construction methods (signal peptides, enzyme targeting, gene knockouts) specifically for engineering 'human-like' glycosylation in expression platforms central...
3 claims
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US-2007184063-A12007
The document details essential strain engineering methods for improving PTM fidelity (specifically humanized N- and O-linked glycosylation) in key expression platforms relevant to the goal (Pichia,...
3 claims
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US-2007231860-A12007
The document focuses on engineering post-translational modifications (PTMs), specifically glycosylation (sialylation), which is a critical aspect of the research goal (PTM fidelity). While the...
3 claims
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US-2007298500-A12007
The document provides detailed strain construction data regarding a novel, regulatable promoter system (P-FLD1) for heterologous gene expression in Pichia pastoris. Pichia is a key expression...
3 claims
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US-2008060092-A12008
The document provides detailed genetic engineering methods (inhibiting FucT and XylT via RNAi constructs) specifically aimed at achieving 'humanized' N-glycosylation patterns (G0 glycoform) for...
3 claims
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US-2008064084-A12008
Although the target protein is porcine trypsin, the document directly addresses core aspects of the research goal by detailing strain construction strategies (signal peptides, propeptide engineering,...
3 claims
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US-2008124763-A12008
The document details vectors and methods for protein expression and efficient secretion using engineered signal peptides (lysozyme leader peptide) in key expression platforms (Pichia pastoris,...
3 claims
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US-2008066200-A12008
The research goal explicitly requires information on PTM fidelity, especially glycosylation. This patent provides highly detailed, specific strain engineering methods (targeting FucT and XylT...
3 claims
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US-2008166762-A12008
The document provides highly specific and transferable methodological data (strain construction details, promoter usage, vector system, quantitative secretion levels, and fermentation strategy) for...
3 claims
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US-2008274498-A12008
The document details essential strain construction methods for engineering lower eukaryotes (methylotrophic yeast like Pichia and fungi) to achieve 'human-like' glycosylation (PTM fidelity). This...
3 claims
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US-2008280324-A12008
The document details specific genetic engineering methods (introducing human GnT-I activity) to modify and improve N-glycosylation fidelity toward a mammalian pattern in Basidiomycetes (a type of...
3 claims
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US-2008299616-A12008
The document details the isolation and application of specific DNA regulatory elements (MLS1 promoter and terminator) for engineering the Pichia pastoris expression platform. This information is...
3 claims
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US-2008311612-A12008
The document utilizes Pichia pastoris, a key target expression platform, and provides specific transferable strain construction details (e.g., constitutive promoters PGAP, pYPT1; strains KM71, GS115,...
3 claims
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US-2008318275-A12008
The document, a patent, directly addresses the limitations and specific outcomes of PTM fidelity (glycosylation, specifically lack of sialylation and presence of mannose-terminated structures) when...
3 claims
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US-2009011466-A12009
The document does not cover the target human milk proteins or the preferred microbial hosts (it uses CHO cells for immune receptor fusion proteins). However, it provides detailed information on...
3 claims
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US-2009074798-A12009
The document details methods and strain engineering data for achieving N-glycosylation (a critical PTM fidelity parameter) in a prokaryotic expression platform (bacteria/E. coli), which is one of the...
3 claims
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US-2009081762-A12009
The document details recombinant protein expression and engineering protocols (host switching, promoter use, yield optimization) specifically within filamentous fungi (Aspergillus, Penicillium,...
3 claims
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US-2009098650-A12009
Although the primary application of the patent is stem cell culture, the Abstract explicitly states that the invention includes 'methods for the expression of recombinant rabbit LIF in the Pichia...
1 claim
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US-2009104693-A12009
The research goal specifically focuses on PTM fidelity (glycosylation) in yeast and bacterial expression systems. This patent details the cloning, vector construction, and successful heterologous...
2 claims
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US-2009181041-A12009
The document details highly relevant strain engineering methods for improving PTM fidelity (humanized glycosylation, specifically O-glycans and elimination of high mannose N-glycans) in key...
3 claims
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US-2009187999-A12009
The document describes detailed genetic engineering constructs, including the use of highly relevant regulatory elements specific to the target proteins listed in the goal (beta casein promoter,...
2 claims
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US-2009280536-A12009
This patent describes a tunable expression system (T7RNAP/T7Lysozyme control) designed to optimize yields and minimize folding stress for complex proteins, including secretory and glycosylated...
3 claims
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US-2009298122-A12009
The document provides detailed technical data from a patent (a targeted source) regarding the construction and optimization of one of the key expression platforms specified in the research goal:...
3 claims
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US-2010028384-A12010
The document provides detailed strain construction data (pGAPZαC, alpha-factor secretion signal) and crucial findings regarding PTM fidelity (N-glycosylation, homodimer formation, conformation) and...
2 claims
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US-2010021991-A12010
The document is a patent detailing methods for engineering specific yeast and fungal expression platforms (Pichia pastoris, Saccharomyces cerevisiae, Aspergillus) to produce glycoproteins with...
3 claims
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US-2010029548-A12010
Although the document focuses on plant expression systems and human growth hormone (HGH), it addresses core methodological concerns of the research goal: engineering post-translational modifications...
3 claims
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US-2010028993-A12010
Although the primary expression platform discussed is plants (not the targeted microbial systems), the document provides detailed technical information relevant to the research goal's focus areas:...
3 claims
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US-2010092613-A12010
The document details the construction of a recombinant expression system using one of the target expression platforms, Pichia pastoris (Claim 8, Abstract), to achieve high specific activity/titer for...
3 claims
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US-2010167998-A12010
The document details the use of Pichia pastoris (one of the required expression platforms) for the extracellular expression and secretion of a bioactive recombinant polypeptide. Although the protein...
3 claims
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US-2010175147-A12010
The document, while expressing a plant protein, focuses extensively on the use of Pichia pastoris (a required expression platform) and provides specific, transferable detailed strain construction...
2 claims
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US-2010273980-A12010
The document details a method for increasing the yield and stability (titer/secretion) of heterologous proteins expressed in Aspergillus (a filamentous fungus, one of the target expression platforms)...
3 claims
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US-2010279345-A12010
The document provides detailed strain construction data (a novel auto-inducible promoter, vector design, and cultivation method) specifically for Pichia pastoris, one of the key expression platforms...
3 claims
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US-2010311122-A12010
The document provides detailed strain engineering methods for improving the production (titer/secretion) and Post-Translational Modification (PTM) fidelity (specifically reduced O-glycosylation) of...
3 claims
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US-2011020870-A12011
The document provides detailed strain engineering methods, specifically focused on modifying key expression platforms (Pichia, Saccharomyces, filamentous fungi) to achieve human-like glycosylation...
3 claims
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US-2011053195-A12011
The patent explicitly discusses and claims the use of the key expression platforms relevant to the research goal (Pichia pastoris, Saccharomyces, bacteria, and various filamentous fungi) for...
2 claims
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US-2011021378-A12011
The document provides detailed strain construction data (genome sequence, novel promoters, signal peptides, and expression vectors) specifically for the Pichia pastoris expression platform. Pichia is...
3 claims
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US-2011136207-A12011
The document provides detailed strain construction data regarding expression platform optimization (Pichia pastoris vs. E. coli), focusing specifically on achieving high titer/secretion using a...
3 claims
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US-2011143396-A12011
The document details strain construction methods (overexpression of Ca2+ ATPase/chaperones, PMT deletion) specifically designed to improve PTM fidelity (reducing O-glycosylation/hypermannosylation)...
3 claims
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US-2011144308-A12011
The document, a patent, provides detailed methodologies for achieving 'humanized' N-glycosylation (PTM fidelity) by engineering the host expression system (plant/duckweed) through gene knockdown...
3 claims
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US-2011154514-A12011
This patent provides detailed strain engineering methods involving signal peptides and glycosylation sequences (N-type and O-type tags) to enhance the secretion and sugar chain modification (PTM...
3 claims
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US-2011230404-A12011
The document does not study the specified microbial expression systems (Pichia, Saccharomyces, bacteria) but rather plants. However, it provides molecular engineering strategies focused on core areas...
3 claims
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US-2011236925-A12011
The document provides detailed strain construction data (mat-alpha signal peptide, Pichia pastoris GS115), discusses secretion, and focuses heavily on the identification and removal of undesirable...
3 claims
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US-2011262619-A12011
Although the target protein is phytase, the document explicitly details the strain construction methodologies required by the research goal. It provides specific examples of promoters (e.g.,...
3 claims
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US-2011312032-A12011
The document details specific strain engineering approaches (PDI and PMT deletion/replacement, Ca2+ ATPase overexpression) in key expression platforms (Pichia pastoris, lower eukaryotes) aimed at...
3 claims
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US-2012003695-A12012
The document focuses on genetically engineering the Pichia pastoris expression platform (one of the target platforms) to improve PTM fidelity, specifically by enabling the production of...
3 claims
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US-2012077966-A12012
Although the target protein is human insulin precursor, not an HMP, this patent provides highly detailed, specific methodological data regarding strain construction (vector choice pPIC9K, signal...
3 claims
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US-2012021488-A12012
Although the target protein is phytase, the document directly compares the expression performance (titer/yield) of multiple key platforms required by the research goal: bacteria (E. coli) and various...
3 claims
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US-2012100556-A12012
The document provides detailed strain construction data (specific promoters like pGAP and pYPT1, vectors, and Pichia cell lines KM71/GS115) for optimizing heterologous protein expression in Pichia...
3 claims
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US-2012295307-A12012
The document details a strain engineering approach (inactivation of the Sub2 protease) in Pichia pastoris, a target expression platform, explicitly designed to increase the stability, integrity, and...
3 claims
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US-2012121630-A12012
Although the target protein is HSV glycoprotein, the document is highly relevant because it provides detailed, transferable technical data concerning strain engineering for one of the core expression...
3 claims
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US-2012315671-A12012
The patent details novel media formulations and methods for cultivating cells, specifically focusing on the optimization of recombinant protein expression in Pichia pastoris. Pichia is one of the key...
3 claims
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US-2012322100-A12012
The document details specific strain engineering methods (gene knockouts, exogenous enzyme expression, targeting signals) for methylotrophic yeast (Pichia/fungi), a key expression platform, aimed at...
3 claims
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US-2012328626-A12012
The document addresses the critical research requirement of PTM fidelity (N-glycosylation) and provides detailed strain engineering methods. It explicitly names the key expression platforms mentioned...
3 claims
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US-2013029413-A12013
The document details genetic engineering methods (strain construction data, specific promoters, metabolic pathways, enzyme sequences) to improve Post-Translational Modification (PTM) fidelity,...
3 claims
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US-2013040897-A12013
The document provides detailed strain construction data (knockouts, introduction of transferases like GnTI, GnTII) focused entirely on achieving human-like glycosylation (PTM fidelity) in yeast...
3 claims
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US-2013237475-A12013
The document provides detailed, transferable platform engineering data for Pichia pastoris (a target platform). It covers strain construction (synthetic gene design, codon optimization, AOX1...
3 claims
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US-2013295604-A12013
The document, a patent detailing strain engineering, directly addresses the crucial research metric of Post-Translational Modification (PTM) fidelity (specifically glycosylation) in key expression...
3 claims
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US-2013316403-A12013
The patent details strain engineering strategies (Ca2+ ATPase overexpression, PMT gene deletion) in key expression platforms (Pichia, Saccharomyces, filamentous fungi) to achieve improved PTM...
3 claims
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US-2014011236-A12014
The document provides detailed strain construction data (a large list of novel promoters and their sequences) optimized for key expression platforms listed in the research goal (Pichia pastoris,...
3 claims
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US-2014024806-A12014
Although the platform is plants (not microbial/fungal) and the specific target proteins are non-HMP, the document provides detailed engineering strategies involving the use of secretory signal...
3 claims
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US-2014051842-A12014
The document, although focused on human insulin rather than human milk proteins, provides highly detailed, practical strain construction and fermentation methodology for optimizing protein secretion...
3 claims
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US-2014057318-A12014
Although the target protein is insulin, the document provides detailed, highly transferable engineering and process data relevant to comparing expression platforms. It specifically details strain...
3 claims
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US-2014186886-A12014
The document details highly relevant strain engineering strategies (co-expression of bacterial enzymes, PNGase F) to manipulate and control N-linked Post-Translational Modifications (PTMs,...
3 claims
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US-2014220061-A12014
Although the target protein (Flavivirus VLP) is different from the research goal (human milk proteins), the document provides highly relevant and detailed strain engineering data for a key expression...
1 claim
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US-2014234902-A12014
The patent provides detailed strain engineering methods, including the use of specific promoters, enzyme deletions (e.g., OCH1 mutant), and enzyme targeting (signal peptides) in relevant expression...
3 claims
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US-2014227290-A12014
The document focuses on improving N-glycosylation site occupancy (PTM fidelity) in key expression platforms relevant to the research goal, specifically yeast (Saccharomyces, Pichia) and filamentous...
3 claims
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US-2014274761-A12014
The document details the engineering and characterization of a novel constitutive promoter (pCS1) in Pichia pastoris and Saccharomyces, aiming to achieve higher expression levels (titer) than...
3 claims
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US-2014288272-A12014
The document details processes (purification, monitoring, process adjustment) specifically aimed at controlling and minimizing glycosylated impurities (glycovariants, N-linked and O-linked) produced...
3 claims
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US-2014302556-A12014
This document details strain engineering strategies (PMT2 mutation) in relevant expression platforms (Pichia, Saccharomyces, filamentous fungi) specifically to control O-glycosylation (PTM fidelity)...
3 claims
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US-2014308699-A12014
The document provides detailed strain construction data (YAC architecture, promoters, signal sequences, chaperones) for engineering improved Post-Translational Modification (PTM) fidelity,...
3 claims
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US-2014308702-A12014
The document provides detailed strain construction data, specific promoters (pGAPDH, pADH1, pGAL1/10), signal sequences (HDEL, Mnn9), and genetic modifications (OCH1/MNN1/MNN9 deletions, introduction...
3 claims
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US-2014335127-A12014
The document details strain engineering methods for achieving N-glycosylation (PTM fidelity) in a prokaryotic platform (E. coli/bacteria), which is one of the target platforms specified in the...
2 claims
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US-2014329276-A12014
This patent details specific strain engineering methods (ALG3/OS-9 disruption, heterologous OTase expression) in key expression platforms (Pichia, Saccharomyces, filamentous fungi) to improve two...
2 claims
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US-2015011407-A12015
The document provides detailed strain construction data, specifically focusing on bidirectional promoter sequences, vector design, and expression optimization methods within Pichia pastoris. Pichia...
3 claims
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US-2015105322-A12015
The document, though concerning Annexin A2 and not a human milk protein, provides detailed, transferable strain construction and engineering data highly relevant to the goal. It specifically...
3 claims
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US-2015017686-A12015
This patent provides detailed strain engineering methods and outcomes for improving Post-Translational Modification (PTM) fidelity (specifically glycosylation) in key expression platforms listed in...
3 claims
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US-2015119325-A12015
Although the host platform (plants) and the target protein (EPO) differ from the research goal (microbial hosts, human milk proteins), the document details highly specific, transferable molecular...
3 claims
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US-2015118710-A12015
Although the target protein is insulin glargine, the document provides highly specific and transferable detailed strain construction data and engineering outcomes regarding optimizing protein...
2 claims
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US-2015166679-A12015
The document focuses on the recombinant production and purification of Secretory Component (SC), a key part of the target human milk protein sIgA. Critically, the patent details strain engineering...
3 claims
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US-2015225472-A12015
The document provides detailed strain construction and process data (as requested in the notes) using two key expression platforms specified in the research goal: Pichia pastoris and Saccharomyces...
3 claims
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US-2015232898-A12015
The document provides detailed strain construction data and industrial production outcomes (high titer, constitutive expression, multi-copy integration, secretion/recovery) using the Pichia pastoris...
3 claims
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US-2015267212-A12015
The research goal requires comparing PTM fidelity (glycosylation) across different expression platforms, including Pichia pastoris. This patent details specific genetic engineering methods, strain...
3 claims
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US-2015275222-A12015
The patent details strain engineering strategies (CRZ1 mutation) in a target platform (Pichia pastoris) to improve yield, robustness, and cell viability, specifically addressing issues caused by...
3 claims
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US-2015284731-A12015
The document provides detailed strain construction data (identification and utilization of a novel promoter) and strain engineering outcomes (expression analysis under various carbon...
3 claims
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US-2015315263-A12015
The document utilizes one of the specified expression platforms (*Pichia pastoris*) to produce recombinant protein fragments (Factor VIII heavy and light chain). Although the protein studied is...
3 claims
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US-2015337274-A12015
The document provides detailed strain engineering data for Pichia pastoris (a target platform) focusing specifically on optimizing PTM fidelity (glycosylation via OCH1/PMT knockouts) to produce...
3 claims
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US-2015353620-A12015
The document provides a detailed process optimization model (using Critical Nutrient Ratio and protease inhibitors) specifically for enhancing recombinant protein titer and secretion (by reducing...
3 claims
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US-2015376249-A12015
This patent provides detailed strain construction methods aimed at improving N-glycosylation site occupancy (PTM fidelity) and yield (titer) for recombinant glycoproteins. The specified host cells...
3 claims
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US-2016024511-A12016
The document provides detailed strain construction data (novel methanol-inducible and constitutive promoters) specifically for the Pichia pastoris expression platform, which is one of the key...
3 claims
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US-2016039891-A12016
This patent provides detailed strain construction data focused on optimizing recombinant protein production (titer/secretion) in key expression platforms listed in the research goal, specifically...
2 claims
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US-2016039924-A12016
The document focuses on modulating the glycosylation profile (specifically mannosylation and galactosylation levels) of recombinant proteins via bioprocess control (dissolved oxygen). PTM fidelity,...
3 claims
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US-2016068880-A12016
This patent details methods for genetically modifying key expression platforms (Pichia, Saccharomyces, Aspergillus, etc.) to achieve human-like glycosylation patterns. This engineering is crucial for...
3 claims
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US-2016097053-A12016
The document describes and patents novel constitutive promoter sequences specifically isolated from Pichia pastoris. Pichia is a target expression platform in the research goal, and the goal...
3 claims
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US-2016168198-A12016
The document is a patent detailing novel strain construction elements (signal sequences and promoters, specifically the Pichia pastoris DDDK signal peptide and AOX1 promoter) and strain engineering...
3 claims
|
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US-2016168556-A12016
The document provides detailed strain engineering data and outcomes (codon modification, culture conditions, and PTM control) specifically within one of the target expression platforms (Pichia...
3 claims
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US-2016237127-A12016
The document details the design and construction of synthetic genes (codon optimization) and expression strategies using one of the required platforms (Pichia pastoris). Although the target protein...
3 claims
|
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US-2016185848-A12016
The document focuses entirely on methods for modulating the glycosylation profile (PTM fidelity) of recombinant proteins, including detailed changes to mannosylation and galactosylation profiles...
3 claims
|
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US-2016264953-A12016
The document focuses heavily on defining and engineering specific Post-Translational Modifications (PTMs), specifically N-linked oligosaccharide profiles (sialylation, phosphorylation, fucose...
2 claims
|
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US-2016355860-A12016
The document provides detailed strain construction and engineering data (CRZ1 mutation, combined with OCH1 and other glycosylation pathway gene modifications) aimed at enhancing the robustness,...
3 claims
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US-2017137500-A12017
The document is highly relevant as it describes specific processes, purification strategies, and analytical methods (lectin binding assays, chromatography) for controlling and minimizing glycosylated...
3 claims
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US-2017166910-A12017
The document provides detailed strain construction data from a patent regarding engineering the Pichia pastoris expression platform (one of the key platforms in the research goal). Specifically, it...
3 claims
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US-2017204389-A12017
The document provides detailed strain construction data, engineering outcomes (mutagenesis for enhanced function), and utilizes specific expression platforms relevant to the research goal, including...
3 claims
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US-2017226552-A12017
The document focuses on a core technical metric of the research goal: modulating protein glycosylation (PTM fidelity) using specific media components (cobalt supplementation). While the host cells...
3 claims
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US-2017306009-A12017
Although the document uses CHO cells to produce adalimumab, its core focus is modulating glycosylation profiles (specifically decreasing high mannose content) via bioprocess parameter control...
3 claims
|
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US-2017355760-A12017
The document addresses methods for controlling and modulating glycosylation profiles (PTM fidelity), which is a crucial aspect of the research goal. Although the specific host (CHO cell) and product...
3 claims
|
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US-2018119193-A12018
The research goal specifically focuses on comparing PTM fidelity (glycosylation) across different expression platforms for human milk proteins. This patent details genetic engineering strategies...
3 claims
|
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US-2018142038-A12018
The document provides critical methodology (anti-mannosylation antibodies) for monitoring and controlling Post-Translational Modification (PTM) fidelity (specifically glycosylation, which is a major...
3 claims
|
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US-2019037820-A12019
Although the platform is an insect (silkworm) rather than the specified yeast/fungi/bacteria, the document details complex strain engineering protocols, specific promoter usage (silk gland...
3 claims
|
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US-2018327764-A12018
Although the document focuses on plant expression systems and vaccine antigens, its core topic is a highly specific technical challenge directly relevant to the research goal: managing and preventing...
3 claims
|
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US-2019085364-A12019
The document is highly relevant because it provides detailed, proprietary information (from a patent) regarding the expression platform (Pichia pastoris), specifically addressing key metrics of the...
3 claims
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US-2019256860-A12019
The document details specific genetic engineering methods in a target expression platform (Pichia pastoris) to control and improve N-glycosylation patterns (PTM fidelity). Controlling glycosylation...
3 claims
|
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US-2019276500-A12019
Although the platform is a viral vector (Yellow Fever 17D) used for vaccine production, the document provides detailed expression cassette engineering data from a patent regarding critical...
2 claims
|
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US-2019337998-A12019
The document provides detailed strain construction data (novel Pichia pastoris promoters, expression constructs, and signal peptides) specifically engineered to maximize constitutive expression...
2 claims
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US-2019389918-A12019
The document provides highly detailed and transferable information regarding strain engineering protocols for a target expression platform (*Pichia pastoris*), including specific vectors (pPIC3.5K,...
3 claims
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US-2019389919-A12019
Although the target protein (Der f1 allergen) is not a human milk protein, the document provides highly detailed, practical strain engineering data crucial for comparing expression platforms. It...
3 claims
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US-2020010515-A12020
The document provides detailed, patent-level strain construction and optimization data (synthetic gene, codon optimization, AOX1 vs. GAP promoter comparison, multi-copy selection) specifically for...
3 claims
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US-2020123523-A12020
Although the target protein (Der p1) is not a human milk protein, the document provides highly specific and transferable detailed strain construction data (codon optimization, specific vectors like...
3 claims
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US-2020032279-A12020
The document is a patent providing detailed strain construction data (promoters OLE1/DAS2, specific signal peptides, integration loci) specifically for maximizing heterologous protein expression and...
3 claims
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US-2020131235-A12020
The document provides highly detailed, specific, and transferable strain construction and engineering data (codon optimization strategy, specific vectors like pPICZ A/pGAPZ A, specific strains like...
3 claims
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US-2020190526-A12020
The document is a patent detailing strain engineering methods in Pichia pastoris (a key expression platform in the research goal) specifically to achieve substantially homogeneous N-glycans...
3 claims
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US-2020215180-A12020
The document, a patent, details the engineering and construction of recombinant proteins specifically focused on introducing novel N-linked glycosylation sites (Asn-X-Ser/Thr sequons) to control...
3 claims
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US-2020223913-A12020
The document details processes for producing and purifying recombinant proteins, specifically focusing on managing glycosylated impurities (PTM fidelity) in key expression platforms relevant to the...
3 claims
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US-2020308593-A12020
The document provides detailed strain construction data (novel constitutive promoters and vectors) specifically for Pichia pastoris, which is a key expression platform mentioned in the research goal....
3 claims
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US-2020385693-A12020
The document provides specific technical details on strain engineering (ade2 deletion), secretion signals (alpha-mating factor), and expression optimization in Pichia pastoris to produce an...
3 claims
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US-2020399646-A92020
The document is a patent providing detailed strain construction data for protein production in Pichia pastoris (a target platform). It details specific promoters (OLE1, DAS2), signal sequences...
3 claims
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US-2021032307-A12021
The document provides detailed strain construction data (codon optimization, specific signal peptides like alpha-factor, vector design) and operational insights focused on enhancing secretion...
3 claims
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US-2021108214-A12021
The document provides detailed strain construction data (using a specific signal peptide, promoters, and vectors) and engineering outcomes for improving secretion and N-terminal processing fidelity...
3 claims
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US-2021355498-A12021
The document is a patent providing detailed molecular tools (novel constitutive promoters, vectors, and host cells) specifically for optimizing protein expression in Pichia pastoris. Pichia is one of...
3 claims
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US-2021388411-A12021
The document details genetic engineering techniques (specific promoters, signal peptides/anchors, vector design) and methods for screening improved protein secretion pathway genes (bip1, hac1, etc.)...
3 claims
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US-2022025422-A12022
The document, a patent, details a specific strain engineering strategy (Ire1 mutation) in a filamentous fungal platform (*Trichoderma reesei*, *Aspergillus*, etc.) to improve the secretion and...
3 claims
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US-2022064617-A12022
The document details specific methods for optimizing and characterizing Post-Translational Modification (PTM) fidelity, specifically N-linked glycosylation (e.g., mannose-6-phosphorylation,...
3 claims
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US-2022112535-A12022
The research goal requires comparing expression platforms regarding PTM fidelity, specifically glycosylation. Although the primary subject is insect cells, the patent focuses entirely on detailed...
3 claims
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US-2022119494-A12022
The document, a detailed patent, focuses on enhancing titer and secretion of a complex human protein (HSA) using one of the key expression platforms (Pichia pastoris) listed in the research goal. It...
2 claims
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US-2022145311-A12022
The document is a patent detailing strain construction and engineering outcomes for Pichia pastoris (one of the target expression platforms). It specifically addresses PTM fidelity (glycosylation) by...
3 claims
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US-2022154236-A12022
The research goal requires detailed strain construction data, especially regarding signal peptides and secretion/titer comparison across platforms, including bacteria. This patent provides specific...
3 claims
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US-2022162543-A12022
The document provides highly detailed strain construction data (specific mutations, deposit numbers) and engineering outcomes for optimizing Pichia pastoris, which is one of the target expression...
3 claims
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US-2022213460-A12022
The document provides detailed strain engineering methods and outcomes for controlling post-translational modifications (specifically achieving an unglycosylated form) and verifying bioactivity of a...
3 claims
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US-2022251621-A12022
The document provides detailed, practical strain construction data (e.g., fusion tags, cleavage sites, and high cell density fermentation protocols) relevant to maximizing titer and secretion in the...
3 claims
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US-2022289810-A12022
Although the target protein (equine CG) and the final successful expression platform (CHO cell) do not match the goal, the document's core focus is highly relevant. It provides detailed, transferable...
3 claims
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US-2022389072-A12022
The document details specific strain engineering techniques (fusion tags, expression sequences, HCD fermentation protocols) designed to maximize recombinant peptide TITER and solubility in key...
3 claims
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US-2023119018-A12023
Although the target protein (Type XVII collagen) is not a human milk protein, the document provides highly relevant and detailed technical information on the expression platform (Pichia pastoris). It...
3 claims
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US-2023127506-A12023
Although the target protein (Interferon-α2) is different, the document provides detailed methodologies from a patent context concerning engineering recombinant proteins for PTM fidelity, specifically...
3 claims
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US-2023167449-A12023
The document describes a specific strain engineering approach (EXT1 depletion/inhibition) intended to improve recombinant polypeptide production in eukaryotic cells by altering ER shaping and...
3 claims
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US-2023220358-A12023
The document provides detailed, practical strain construction data (signal peptides, vectors, promoters like AOX1/GAP), host cell engineering outcomes, and methods for achieving high yield and...
2 claims
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US-2023227804-A12023
The document, a patent, provides highly detailed strain construction data (specific promoters, vectors, and multiple signal peptides: FAK, AT, IV, etc.) and engineering outcomes aimed at achieving...
3 claims
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US-2023203556-A12023
The patent details methods for producing human milk proteins (caseins, lactoferrin, alpha-lactalbumin) and achieving crucial Post-Translational Modification (PTM) fidelity (phosphorylation via...
3 claims
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US-2023272046-A12023
The document, a patent detailing recombinant protein expression in *Pichia pastoris*, provides specific and detailed strain construction data (vectors: pPICZαA, pHIL; host strains: GS115, X33; use of...
3 claims
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US-2023295594-A12023
The document details specific, transferable methodologies for strain construction and high-density fermentation protocols (titer, secretion efficiency) using one of the key expression platforms...
3 claims
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US-2023322866-A12023
Although the host is a diatom (Phaeodactylum tricornutum) and the product is a viral antigen, the document provides detailed strain engineering data relevant to the core aspects of the research goal:...
3 claims
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US-2023340507-A12023
The document describes detailed strain construction data (novel constitutive promoters) and expression methods specifically for the Pichia pastoris platform, which is one of the key expression...
3 claims
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US-2023399395-A12023
The document provides explicit, high-quality, quantitative data (titer of 13 g/L and high purity/secretion) and engineering details (host cell, vector, production method) for one of the key...
3 claims
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US-2024035036-A12024
The document details a method for engineering bacterial systems (Gram-negative hosts) to perform specific O-based glycosylation using FlmG glycosyltransferases. This directly addresses two major...
3 claims
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US-2024132863-A12024
Although the specific target protein is DNase instead of a human milk protein, the document directly addresses the core research requirements concerning expression platforms. It provides detailed...
3 claims
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US-2024150742-A12024
The document provides detailed, transferable technical data concerning one of the target expression platforms (*Pichia pastoris*). It specifically addresses strain construction data (signal peptide...
3 claims
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US-2024191274-A12024
The research goal requires detailed strain construction data, specifically focusing on signal peptides and secretion optimization in bacterial platforms. This document provides successful, detailed...
3 claims
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US-2024228995-A92024
Although the target protein (D1L2 enzyme) is not a Human Milk Protein, the document directly addresses the core methodological requirements of the research goal. It provides detailed strain...
3 claims
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US-2024262876-A12024
The document provides detailed, practical data on strain construction and expression optimization (synthetic gene design, use of AOX1 and GAP promoters, and large-scale fermentation results)...
3 claims
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US-2024352472-A12024
The document details novel genetic engineering strategies in the Pichia pastoris expression platform to achieve substantially homogeneous N-glycans (Man8GlcNAc2 or Man5GlcNAc2) by mutating the OCH1...
3 claims
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US-2024344099-A12024
The document details specific strain construction and engineering outcomes (promoters AOX1p, DAS2p, FLD1p; codon optimization; flocculation gene introduction using FLO1) applied to a key expression...
3 claims
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US-2024368264-A12024
The document provides specific high-titer data (17-23 g/L) for recombinant protein production using Pichia pastoris, which is one of the key expression platforms specified in the research goal. The...
2 claims
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US-2025025546-A12025
The document, a patent, uses the Pichia pastoris expression system, which is one of the platforms listed in the research goal. Although the expressed protein is a norovirus VLP (not a human milk...
2 claims
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US-2025059526-A12025
The document, a patent, details the methodology for expressing a bioactive protein (Lysostaphin) in a key expression platform specified in the research goal (Pichia pastoris). Crucially, it focuses...
3 claims
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US-2025122518-A12025
While the platform described is plant-based (plant T-DNA vectors), the document provides highly detailed, patented methods for strain engineering focused specifically on controlling and optimizing...
3 claims
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US-2025250325-A12025
The document provides detailed, strain-specific engineering data (OCH1 modification) and processing methods (continuous flow) for precisely controlling N-glycosylation in Pichia pastoris. Although...
3 claims
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US-2025197465-A12025
The document, while focusing on TGFβ, provides detailed, transferable knowledge highly relevant to the research goal's core parameters. It discusses specific expression platform comparisons (Pichia...
3 claims
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US-2025261668-A12025
The document describes the production of lysozyme (a target human milk protein) using recombinant host cells and expression vectors. This directly relates to the research goal of comparing expression...
3 claims
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US-2025263679-A12025
The document details a specific strain engineering technique (mutant RNase E co-expression) intended to enhance the recombinant expression titer of difficult proteins in prokaryotic microbial hosts...
2 claims
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US-2025304980-A12025
The document provides detailed strain construction data for engineering Pichia pastoris (a key platform in the research goal) specifically to improve N-glycosylation patterns (PTM fidelity)....
3 claims
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US-2025320479-A12025
Although the target protein is FTase, the document provides extensive, highly specific, and transferable engineering data directly addressing the research goal's requirements. It details strain...
3 claims
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|
US-4775624-A1988
The document details recombinant DNA expression strategies, vector construction (promoters, signal peptides via R/R1 sequences), and the engineering of human proteins in various expression platforms...
3 claims
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US-4885252-A1989
The document details the cloning and expression strategies, including specific promoters, terminators, and vector constructions (plasmids pOGO4, pOGO216), for heterologous protein production in...
3 claims
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US-4935349-A1990
The document is a patent describing detailed methods and strain construction data (promoters, integration systems) for expressing heterologous eukaryotic genes in filamentous fungi (Aspergillus)....
3 claims
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US-5070020-A1991
The document is a patent that details strain construction, specific promoters (e.g., lambda pL, IPNS, amdS), and vector systems (e.g., pPS63-72) for high-level expression in key platforms relevant to...
3 claims
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US-5151268-A1992
The document, a patent, details the construction of recombinant DNA expression vectors for a heterologous human protein in both prokaryotic and eukaryotic host systems, and specifically addresses the...
3 claims
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US-5177193-A1993
The document focuses on engineering complex human glycoproteins (hormones) for enhanced secretion, dimerization, and controlled glycosylation (PTMs). Although the target proteins and specific host...
3 claims
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US-5223422-A1993
The document, while focusing on t-PA and using CHO cells (mammalian), provides detailed methods concerning the expression, optimization, secretion, and glycosylation assessment of a complex...
2 claims
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US-5296366-A1994
Although the target protein (Streptokinase) is incorrect, the document provides detailed, practical strain construction information (promoters, vectors, specific host strains like Pichia pastoris and...
3 claims
|
|
US-5270040-A1993
Although the target protein is human protein C (a plasma protein) rather than a human milk protein, the document directly addresses core aspects of the research goal: comparing expression platforms...
3 claims
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|
US-5330901-A1994
The document focuses on optimizing the expression and secretion of a heterologous human protein (HSA) in one of the target expression platforms (Pichia pastoris). It provides detailed, transferable...
3 claims
|
|
US-5338835-A1994
Although the target proteins are human reproductive hormones, the document provides detailed information on core technical challenges critical to the research goal: regulating complex...
3 claims
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US-5356804-A1994
The document, while focusing on a different product (alpha-Gal A) and a non-target host (mammalian CHO cells), provides detailed methods and results critical to the research goal. Specifically, it...
3 claims
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|
US-5393670-A1995
The document provides specific and transferable strain engineering data relevant to two key expression platforms (Saccharomyces yeast and filamentous fungi T. reesei source genes). It includes...
2 claims
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US-5447862-A1995
The document provides specific, detailed strain construction data, including characterized promoters (pelA, pelD) and signal peptides (SEQ IDs) for highly efficient secretion in a key expression...
3 claims
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US-5401650-A1995
The document, while focusing on a mammalian host (CHO) and a different protein (alpha-Gal A), provides detailed methodologies and results highly relevant to the core analytical requirements of the...
3 claims
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US-5500483-A1996
The document provides detailed strain construction data by characterizing novel, high-expression regulatory sequences (ZZA1 and ZZA2 promoters) from Pichia pastoris. Pichia is a key expression...
3 claims
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US-5529919-A1996
The document provides detailed methods (patents/claims) on strain construction, vector design, and successful strategies for the expression and secretion of a complex heterologous protein in a...
2 claims
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|
US-5536661-A1996
This document describes detailed strain construction methods, specific promoters (TAKA-amylase promoter), and expression systems for a relevant platform (filamentous fungi, specifically Aspergillus...
3 claims
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US-5545536-A1996
Although the target protein is GM-CSF (not a human milk protein), the document directly addresses core aspects of the research goal: comparing eukaryotic expression platforms (Saccharomyces...
3 claims
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US-5541094-A1996
The document details the engineering and utilization of specific expression platforms (Pichia pastoris, Hansenula polymorpha, Aspergillus nidulans, Escherichia coli) that are central to the research...
3 claims
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US-5550045-A1996
Although the target protein is rhamnogalacturonase, the document provides detailed genetic engineering methods (promoters, regulatory regions, host cell construction, and secretion strategies)...
3 claims
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US-5571697-A1996
The document describes the detailed strain construction (plasmids, promoters, signal peptides, Kex2 cleavage sites) for producing human milk protein (lactoferrin) fragments in a target expression...
3 claims
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US-5591620-A1997
The document details strain construction (recombinant DNA technology) and overexpression methods for producing an industrial protein using Aspergillus, a key filamentous fungal expression platform...
3 claims
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US-5580757-A1996
The document describes the high-yield production, secretion optimization, and detailed analysis of Post-Translational Modification (PTM) fidelity (glycosylation, phosphorylation, processing) for a...
3 claims
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US-5602004-A1997
The document details strain engineering methods and outcomes (promoters, markers, host modification) for optimizing productivity and large-scale fermentation characteristics (titer, secretion...
3 claims
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US-5604129-A1997
The document focuses on engineering and characterizing filamentous fungal platforms (a platform specified in the goal) to achieve improved productivity and titer in large-scale fermentation by...
3 claims
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US-5612198-A1997
The document provides highly detailed, practical data regarding strain construction and engineering outcomes (promoters, signal sequences, multi-copy integration, protease deficient strains like...
3 claims
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US-5624834-A1997
The research goal requires detailed strain construction data from patents regarding expression in filamentous fungi (like Aspergillus). This document provides detailed information on vector...
3 claims
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US-5637491-A1997
The document, a patent, provides detailed engineering outcomes relevant to one of the target expression platforms (Pichia pastoris). It specifically details the use of specific promoter regions and...
3 claims
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US-5641661-A1997
The document provides detailed strain construction data and regulatory elements (ZZA1/ZZA2 promoters, SEQ IDs) for high-level heterologous protein expression and secretion in Pichia pastoris and...
3 claims
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US-5665585-A1997
The document provides detailed strain construction data (specific promoters/terminators like cbh1, secretion signals, vector construction, and host modification) for the filamentous fungi platform...
3 claims
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US-5695985-A1997
The document provides detailed strain engineering and comparative data (titer, viscosity, morphology) for novel filamentous fungal expression platforms (Thermophilic fungi) intended for high-yield...
3 claims
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US-5712142-A1998
The document provides detailed strain construction data (vectors, signal sequences, regulatory sequences, genetic modification approaches) within one of the target expression platforms (*Pichia...
3 claims
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US-5707828-A1998
The document provides specific, detailed strain construction and optimization data (AOX1 promoter, vector design, secretion pathway engineering, codon optimization) for the Pichia pastoris platform,...
3 claims
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US-5714377-A1998
The document describes methods for engineering key expression platforms (Pichia, Saccharomyces, filamentous fungi) to reduce unwanted O-glycosylation (a critical PTM fidelity concern) to produce high...
2 claims
|
|
US-5766912-A1998
The document provides detailed strain construction data (promoters, vectors) for protein expression in a target platform (filamentous fungi, specifically Aspergillus oryzae). Crucially, it analyzes...
3 claims
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US-5795611-A1998
This patent directly addresses the research goal by focusing on the recombinant production of two target human milk proteins (alpha-lactalbumin and beta-casein) in key expression platforms (*E. coli*...
3 claims
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US-5821350-A1998
This patent details methods for producing a recombinant enzyme using Aspergillus niger (a filamentous fungus) and other platforms (yeast, E. coli). Filamentous fungi are a core expression platform...
3 claims
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US-5824528-A1998
The document provides highly detailed strain engineering data, specific promoters (T7, lacO), and regulatory control mechanisms (T7 lysozyme inhibition, lac repression) essential for optimizing the...
2 claims
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US-5827684-A1998
The document, while focusing on Bacillus toxin, provides highly specific and transferable detailed strain construction data for one of the required expression platforms, Pichia pastoris. It details...
3 claims
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US-5830737-A1998
The document is a patent detailing the cloning, vector construction, and expression methodology for a recombinant protein (exo-polygalacturonase) in a filamentous fungal host (Aspergillus). Although...
3 claims
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US-5858704-A1999
While the expression platform is mammalian (293 cells) rather than the specified microbial systems (Pichia, Saccharomyces, bacteria), the document provides highly relevant detailed patent data on...
3 claims
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US-5863759-A1999
The document details strain construction, promoters (TAKA amylase), signal peptides (secretion preregions), and post-translational modification outcomes (specifically glycosylation differences...
3 claims
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|
US-5866391-A1999
The document utilizes and provides detailed strain construction data (vectors, SEQ IDs, background information on expression strategy) for a required expression platform (Aspergillus/filamentous...
3 claims
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US-5871990-A1999
The document provides detailed genetic and vector construction information for UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T3), an enzyme critical for...
3 claims
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US-5871991-A1999
The document provides detailed strain construction data, vector maps, and metabolic engineering strategies for optimizing heterologous protein expression (specifically stability and yield/titer) in a...
3 claims
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|
US-5874558-A1999
The document details a process, including vector systems, promoters (TAKA-amylase), and selection markers, for expressing heterologous proteins in a filamentous fungus (Aspergillus oryzae), which is...
3 claims
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|
US-5879919-A1999
The document, being a patent, provides detailed strain construction data (promoters, enhancers, selection methods) and discusses optimization strategies to achieve novel Post-Translational...
3 claims
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|
US-5942234-A1999
Although the target protein is HCV E1/E2 and not a human milk protein, the document directly addresses the central technical challenge of the research goal: managing Post-Translational Modification...
3 claims
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US-5955316-A1999
The document details the production of a target human milk protein (lactoferrin) using one of the specified expression platforms (Aspergillus/filamentous fungi). It provides crucial data on strain...
3 claims
|
|
US-5942274-A1999
This patent directly addresses the recombinant production of two target human milk proteins (alpha-lactalbumin and beta-casein) in specific microbial expression platforms (*E. coli* and *S....
3 claims
|
|
US-5958747-A1999
The document, a patent detailing strain construction, directly relates to the target expression platforms (Saccharomyces, filamentous fungi, bacteria) and addresses core research metrics like titer...
3 claims
|
|
US-5965384-A1999
The document provides detailed strain construction data (promoters, vectors, markers) and outcomes (glycosylation fidelity and improved stability/resistance) for heterologous protein expression in a...
3 claims
|
|
US-5965389-A1999
The document provides detailed strain construction data (AOX1 promoter, terminator selection), quantitative production outcomes (titer >500 mg/L), and functional fidelity assessment for a recombinant...
3 claims
|
|
US-6013489-A2000
Although the target protein (rhamnogalacturonase) is not a human milk protein, the document provides detailed strain construction data, specifically focusing on expression strategies (regulatory...
3 claims
|
|
US-6020015-A2000
The patent focuses entirely on the production and use of recombinant bioactive human milk proteins (lactoferrin, lysozyme, sIgA, alpha-lactalbumin, caseins) for infant nutrition, which directly...
3 claims
|
|
US-6057130-A2000
The document, although focused on mammalian cells (293 cells) rather than microbial systems, contains detailed strain construction methodology (use of specific viral enhancers/promoters like BK and...
3 claims
|
|
US-6074846-A2000
The document, although focused on HCV proteins, provides detailed technical findings comparing expression platforms (lower eukaryotes/fungi vs. mammalian cells) regarding Post-Translational...
3 claims
|
|
US-6074852-A2000
The document discusses the specific PTM fidelity limitations (lack of terminal sialylation resulting in mannose-terminated glycosylation) when expressing glycoproteins in lower eukaryotes (yeast and...
3 claims
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|
US-6080559-A2000
The document describes the production of a target human milk protein (lactoferrin) using one of the specified expression platforms (filamentous fungi: Aspergillus). It provides detailed information...
3 claims
|
|
US-6136561-A2000
The document provides detailed strain construction data from a patent (using specific secretion signal sequences and truncation strategies) aimed at solving problems related to protein secretion,...
3 claims
|
|
US-6165477-A2000
The document focuses on the challenges of producing and secreting a recombinant glycoprotein (Dengue E protein) with proper Post-Translational Modifications (PTMs), specifically folding, disulfide...
3 claims
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US-6222094-B12001
The document focuses on detailed expression system construction (promoters, signal peptides, introns) for the production and secretion of a core research target protein (human kappa-casein) while...
3 claims
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US-6232094-B12001
The document focuses on producing a specific bioactive human milk protein (kappa-casein) for infant nutrition, aligning with the core research topic. It provides detailed genetic construct...
3 claims
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US-6270827-B12001
The patent directly addresses the utilization and necessity of producing recombinant human milk proteins (lactoferrin, lysozyme, sIgA, caseins, alpha-lactalbumin) for infant nutrition, which aligns...
2 claims
|
|
US-6274148-B12001
Although the target protein is HCV, the document directly addresses the core technical challenge of the research goal: comparing expression platforms and methods (including 'lower eukaryotes') to...
3 claims
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|
US-6306630-B12001
The document provides detailed strain construction data, vectors, and host systems (Saccharomyces, filamentous fungi, bacteria) that are central to the research goal. While the expressed protein is...
3 claims
|
|
US-6309868-B12001
Although the target protein is not a human milk protein, this patent details specific strain construction and overexpression methods in two core expression platforms listed in the research goal:...
3 claims
|
|
US-6287866-B12001
The document, a patent, directly focuses on designing stable expression constructs and methods for producing recombinant human beta-casein (a target human milk protein) in bacterial (E. coli) and...
3 claims
|
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US-6342375-B12002
Although the target protein is Human Growth Hormone (HGH) instead of a Human Milk Protein, the document provides detailed, high-quality engineering data (promoter choice, S. cerevisiae alpha factor...
3 claims
|
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US-6410264-B12002
The document provides detailed strain construction data, specifically novel regulatory nucleotide sequences (promoters, terminators) and secretion leader sequences for enhancing protein expression...
3 claims
|
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US-6482607-B12002
The document, while focusing on a non-target host system (Dictyostelium discoideum), provides highly relevant engineering details essential for the research goal. It discusses optimizing expression...
3 claims
|
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US-6730499-B12004
The document provides detailed strain construction data (novel promoters, selectable markers, induction methods) for optimizing heterologous gene expression in Pichia pastoris, which is one of the...
2 claims
|
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US-6605450-B12003
The document provides detailed strain construction and engineering data (promoters, transcriptional regulation via Fe concentration) specifically for key expression platforms mentioned in the...
3 claims
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US-6780615-B12004
The document provides detailed strain construction data (promoters and signal sequences/peptides) specifically comparing expression and secretion mechanisms in two of the target expression platforms:...
2 claims
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US-6838435-B12005
The document describes the recombinant preparation of complex human peptides (Thrombocidins) and explicitly mentions cloning and amplification for large-scale production. While the peptides are not...
2 claims
|
|
US-6867037-B12005
The document provides detailed strain construction data (AOX1 promoter, S. cerevisiae alpha factor signal sequence) and engineering outcomes for secretory expression in Pichia pastoris. It directly...
3 claims
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|
US-7029872-B22006
The document focuses entirely on engineering glycosylation pathways in key expression platforms specified in the research goal (Pichia, Saccharomyces, filamentous fungi/Aspergillus, Trichoderma) to...
3 claims
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|
US-7087808-B22006
The document describes the stable expression, secretion, and bioactivity of a core target protein (Lactoferrin) in a mammalian system (transgenic swine). Although the expression platform is not...
3 claims
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US-7048930-B22006
Although the target protein is HCV, the document directly addresses core research goals related to expression platform comparison and PTM fidelity. It details the use of relevant yeast strains...
3 claims
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US-7091032-B22006
The document, a patent focused on producing human insulin precursor in the target platform Pichia pastoris, provides highly transferable strain construction details (DNA constructions, secretion...
3 claims
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US-7105303-B22006
Although the target protein is HCV, the document contains highly relevant, transferable information comparing the Post-Translational Modification (PTM) fidelity of 'lower eukaryotes' (yeast/fungi...
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US-7238507-B22007
The document details molecular cloning, cDNA isolation, and methods for expressing a complex enzyme system (P450 and oxidoreductase) derived from a filamentous fungus (Aspergillus), which is one of...
3 claims
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US-7250502-B22007
The document provides detailed strain construction data, specifically focusing on alternative promoters (P_FLD1 vs P_AOX1) and selectable markers for Pichia pastoris. Pichia is one of the key...
2 claims
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US-7229962-B22007
The document, a patent on recombinant protein production, discusses critical engineering challenges relevant to the research goal, including strategies for achieving high production yield, improving...
3 claims
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US-7262038-B22007
The document provides highly specific and detailed strain construction data, expression methods, and performance outcomes (high yield, activity) for one of the target expression platforms, Pichia...
3 claims
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US-7276605-B22007
The document provides detailed strain construction and engineering outcomes (signal peptides, propeptide manipulation, pH control during induction) specifically focused on maximizing secretion and...
3 claims
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US-7326681-B22008
This patent details methods for genetically modifying lower eukaryotes (specifically Pichia and filamentous fungi, which are target platforms) to achieve human-like glycosylation patterns on...
3 claims
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US-7368274-B22008
The document, while focusing on Proteinase K, provides highly detailed, transferable information directly relevant to the core methodological aspects of the research goal. Specifically, it details...
3 claims
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US-7449316-B22008
The research goal requires detailed information on PTM fidelity, especially glycosylation, for human milk protein production. This patent provides detailed molecular engineering data (cloning, host...
3 claims
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US-7449550-B22008
The document focuses on producing cellulases, not human milk proteins. However, it provides practical, patent-level details on using one of the required expression platforms (filamentous fungi,...
2 claims
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US-7517668-B12009
The document details the engineering and expression platform (Aspergillus oryzae, a filamentous fungus) which is one of the platforms listed in the research goal. It specifically provides relevant...
3 claims
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US-7629163-B22009
The document provides detailed strain construction data, specifically focusing on the genetic engineering of yeast (Pichia pastoris is explicitly mentioned) and filamentous fungi to achieve...
3 claims
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US-7666629-B22010
Document details the successful optimization of secretion and yield for a complex mammalian protein (porcine trypsinogen) in Pichia pastoris, a key target platform. It provides transferrable...
3 claims
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US-7667089-B22010
The document, although focused on transgenic animal production of FVIII, provides specific details on regulatory elements (promoters and signal peptides) derived from two target human milk proteins...
3 claims
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US-7670816-B22010
The research goal requires data on PTM fidelity (glycosylation) in specific expression platforms (bacteria, yeast). This patent details the isolation, cloning, vector construction, and expression of...
3 claims
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US-7745200-B22010
The document provides detailed strain construction and regulatory data essential for one of the key expression platforms (Pichia pastoris). It describes the formaldehyde dehydrogenase (FLD) promoter...
3 claims
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US-7816509-B22010
The document is a patent detailing strain construction data, specifically a novel constitutive promoter (TEF promoter) for Pichia pastoris. Pichia is one of the target expression platforms, and the...
3 claims
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US-7910330-B22011
The document utilizes a key expression platform specified in the goal (Pichia pastoris) and details critical strain engineering strategies relevant to the research metrics, specifically codon...
3 claims
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US-7884264-B22011
The document details specific genetic engineering methods (RNAi constructs, gene targets FucT and XylT, sequence IDs) used to achieve 'humanized' N-glycosylation profiles in heterologous proteins....
3 claims
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US-8080389-B22011
The document provides detailed strain construction data (a novel auto-inducible promoter) and engineering methodology specifically for one of the target expression platforms, Pichia pastoris. This...
2 claims
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US-7981660-B22011
The document details specific strain engineering methods (promoters, signal peptides, enzyme additions/deletions, e.g., OCH1 mutant) applied to yeast and filamentous fungal expression platforms...
3 claims
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US-8138324-B22012
The document provides a novel, tunable expression system (T7RNAP/T7Lys) designed specifically to optimize the production of recombinant proteins requiring complex post-translational modifications...
3 claims
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US-8211691-B22012
The document, a patent, details the necessary strain engineering and specific genetic modifications (signal peptides, enzyme knockouts, introduction of exogenous transferases) required to achieve...
3 claims
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US-8283150-B22012
The document, a patent, provides detailed technical information on the heterologous expression, strain construction, and comparative performance of a recombinant protein (Cel7A) using one of the...
3 claims
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US-8377676-B22013
The document provides specific, detailed strain engineering methods and outcomes (using a mutated α-factor secretion signal sequence and codon optimization) to dramatically increase the titer and...
3 claims
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US-8440456-B22013
The document provides detailed methods and specific genetic components (promoters, signal peptides, chaperones, glycosylation enzymes) for engineering the Pichia pastoris expression platform. Pichia...
3 claims
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US-8507224-B22013
The document is a patent detailing strain engineering methods for the Pichia expression platform (one of the target platforms) aimed at modifying Post-Translational Modifications (PTM) by reducing or...
3 claims
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US-8455620-B22013
The document details the production methodology, recombinant vector use, and high-level expression outcomes of a heterologous protein (phytase) in one of the key expression platforms specified in the...
2 claims
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US-8575322-B22013
The document is a patent providing detailed, practical strain construction and process engineering data (vectors, signal peptides, fermentation, in-situ capture, DSP) for Pichia pastoris. Pichia is...
3 claims
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US-8633010-B22014
The document details strain engineering methods (co-expression of a PepC inhibitor) in a filamentous fungal platform (Aspergillus) to improve the yield, stability, and recovery (titer and secretion)...
3 claims
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US-8703444-B22014
The document details strain engineering methods (protease knockout/inactivation of Sub2) in a key expression platform, Pichia pastoris, specifically to improve the TITER and recovery of secreted...
3 claims
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US-8652820-B22014
The document, a patent, provides detailed strain construction data (vectors, specific strains, codon optimization) and directly compares the performance (high yield/titer) of key expression platforms...
3 claims
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US-8703471-B22014
The document details a key method for achieving PTM fidelity (N-glycosylation) in one of the target expression platforms (bacteria/Escherichia coli). The research goal specifically asks how bacterial...
2 claims
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US-8715963-B22014
The document provides detailed strain engineering methods (OTase overexpression, och1 mutants) to improve N-glycosylation site occupancy and facilitate human-like glycosylation in key expression...
3 claims
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US-8771989-B22014
The document details specific strain engineering methods (gene deletions like PMT, overexpression of Ca2+ ATPase/chaperones) in a target expression host (Pichia pastoris) to optimize...
3 claims
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US-8802816-B22014
The document provides specific data on using a key expression platform (Pichia pastoris), details strain construction (leader peptides, signal peptides like mat-alpha), and, most critically, focuses...
3 claims
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US-8846373-B22014
The document provides detailed strain engineering methods (nucleic acids, promoters, enzymes) to enhance sialylation (a critical PTM) in recombinant glycoproteins, specifically targeting fungal and...
3 claims
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US-8871907-B22014
The document is a patent detailing specific amino acid modifications (strain construction data) used to engineer and control post-translational modifications (PTM fidelity, specifically...
2 claims
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US-8962811-B22015
Although the platform is plant-based, not microbial, the document details engineering strategies (fusion with specific glycomodules/tags and signal sequence optimization) to drastically increase...
3 claims
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US-9006410-B22015
Although the expression platform is plants (not fungi/bacteria), the document provides detailed strain construction data regarding the engineering of post-translational modifications (glycosylation...
3 claims
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US-9150870-B22015
The document provides detailed strain construction and engineering data (a novel constitutive promoter) specifically for key expression platforms listed in the research goal (Pichia pastoris,...
3 claims
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US-9193772-B22015
The document provides highly detailed, practical strain construction and optimization data for the Pichia pastoris expression platform, which is one of the key platforms in the research goal. It...
3 claims
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US-9314500-B22016
The document provides specific, detailed technical methods (codon optimization, site-specific mutagenesis, purification protocols) for managing Post-Translational Modifications (specifically...
3 claims
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US-9388227-B22016
The document, while focusing on insulin glargine, provides highly detailed, practical strain engineering data in a key expression platform (Pichia pastoris). It addresses the core metrics of the...
2 claims
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US-9404129-B22016
Although the document produces resveratrol, not human milk proteins (HMPs), it provides detailed strain engineering data, production methods, and yield information (titer) across several key...
3 claims
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US-9416389-B22016
The document provides detailed strain engineering methods (VRG4, ATT1, OCH1 disruption) in a key expression platform, Pichia pastoris, specifically to reduce high mannose content and achieve...
3 claims
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US-9428784-B22016
The document focuses on detailed strain engineering methods (gene disruptions, heterologous enzyme expression) in the Pichia pastoris expression platform to increase glycoprotein yield and improve...
2 claims
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US-9518082-B22016
The document focuses on processes for producing recombinant multi-subunit proteins in Pichia pastoris and filamentous fungi (two platforms listed in the research goal). Crucially, the patent details...
2 claims
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US-9518100-B22016
The document provides detailed strain construction data (gene knockouts, overexpression) and engineering outcomes focused on improving N-glycosylation site occupancy and achieving human-like complex...
3 claims
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US-9617550-B22017
The document details strain engineering protocols in Pichia pastoris (a target expression platform) specifically designed to improve N-glycosylation fidelity (PTM fidelity) by generating homogeneous...
3 claims
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US-9663772-B22017
The document is a patent detailing the construction and methods for heterologous protein expression using a key platform specified in the Research Goal: filamentous fungi (Trichoderma). Although the...
3 claims
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US-9834793-B22017
Although the document focuses on resveratrol production, it provides specific, detailed strain engineering data in relevant host organisms (Saccharomyces cerevisiae, E. coli, Aspergillus)....
3 claims
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US-9822357-B22017
The document details proprietary promoter sequences and expression cassette construction specifically for optimizing heterologous protein expression in Pichia pastoris, which is one of the key...
3 claims
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US-9708387-B22017
The document details the specific use and engineering of two required expression platforms (Pichia pastoris and Saccharomyces cerevisiae) for producing a complex recombinant human protein (Factor...
3 claims
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US-9862958-B22018
The document focuses on identifying and utilizing a novel promoter (Sorbitol Dehydrogenase promoter) for heterologous protein expression in Pichia pastoris. P. pastoris is a key expression platform...
3 claims
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